Effects of miR-185-5p on replication of hepatitis C virus
This article was designed to explore the effects and mechanisms of miR-185-5p on the replication of hepatitis C virus (HCV). Quantitative reverse transcription PCR (qRT-PCR) was performed for detecting the abundance of miR-185-5p and HCV RNA in HCV-infected primary hepatocytes and Huh7.5 cells. Dual...
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2021-08-01
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Online Access: | https://doi.org/10.1515/biol-2021-0067 |
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doaj-a41b464e3a6846bf95243c4235653eda2021-10-03T07:42:26ZengDe GruyterOpen Life Sciences2391-54122021-08-0116175275710.1515/biol-2021-0067Effects of miR-185-5p on replication of hepatitis C virusHuang Wei0Song Lingyan1Zhang Jingyan2Yan Xueqiang3Yan Hui4Department of Laboratory Medicine, Heping Hospital Affiliated to Changzhi Medical College, No. 110 Yan’an Nan Road, Changzhi 046000, ChinaDepartment of Laboratory Medicine, Heping Hospital Affiliated to Changzhi Medical College, No. 110 Yan’an Nan Road, Changzhi 046000, ChinaDepartment of Laboratory Medicine, Heping Hospital Affiliated to Changzhi Medical College, No. 110 Yan’an Nan Road, Changzhi 046000, ChinaDepartment of Laboratory Medicine, Heping Hospital Affiliated to Changzhi Medical College, No. 110 Yan’an Nan Road, Changzhi 046000, ChinaDepartment of Laboratory Medicine, Heping Hospital Affiliated to Changzhi Medical College, No. 110 Yan’an Nan Road, Changzhi 046000, ChinaThis article was designed to explore the effects and mechanisms of miR-185-5p on the replication of hepatitis C virus (HCV). Quantitative reverse transcription PCR (qRT-PCR) was performed for detecting the abundance of miR-185-5p and HCV RNA in HCV-infected primary hepatocytes and Huh7.5 cells. Dual-luciferase reporter gene assay was used for exploring the interaction between miR-185-5p and GALNT8. Western blot analyzed protein expression of GALNT8, NS3, and NS5A. miR-185-5p was remarkably downregulated in HCV-infected primary hepatocytes and Huh7.5 cells. miR-185-5p upregulation inhibited HCV RNA expression, while its inhibition promoted HCV replication. miR-185-5p induced accumulation of NS3 and NS5A in the cells. Dual-luciferase reporter gene assay verified the targeted relationship between miR-185-5p and GALNT8. In addition, the effects of overexpressing or knocking down miR-185-5p on HCV replication could be correspondingly eliminated by the overexpression or knockdown of GALNT8. miR-185-5p may target GALNT8 in JFH1-infected Huh7.5 cells and then inhibit HCV replication. miR-185-5p may be a potential target for treating HCV.https://doi.org/10.1515/biol-2021-0067hepatitis c virusreplicationmir-185-5pgalnt8 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Huang Wei Song Lingyan Zhang Jingyan Yan Xueqiang Yan Hui |
spellingShingle |
Huang Wei Song Lingyan Zhang Jingyan Yan Xueqiang Yan Hui Effects of miR-185-5p on replication of hepatitis C virus Open Life Sciences hepatitis c virus replication mir-185-5p galnt8 |
author_facet |
Huang Wei Song Lingyan Zhang Jingyan Yan Xueqiang Yan Hui |
author_sort |
Huang Wei |
title |
Effects of miR-185-5p on replication of hepatitis C virus |
title_short |
Effects of miR-185-5p on replication of hepatitis C virus |
title_full |
Effects of miR-185-5p on replication of hepatitis C virus |
title_fullStr |
Effects of miR-185-5p on replication of hepatitis C virus |
title_full_unstemmed |
Effects of miR-185-5p on replication of hepatitis C virus |
title_sort |
effects of mir-185-5p on replication of hepatitis c virus |
publisher |
De Gruyter |
series |
Open Life Sciences |
issn |
2391-5412 |
publishDate |
2021-08-01 |
description |
This article was designed to explore the effects and mechanisms of miR-185-5p on the replication of hepatitis C virus (HCV). Quantitative reverse transcription PCR (qRT-PCR) was performed for detecting the abundance of miR-185-5p and HCV RNA in HCV-infected primary hepatocytes and Huh7.5 cells. Dual-luciferase reporter gene assay was used for exploring the interaction between miR-185-5p and GALNT8. Western blot analyzed protein expression of GALNT8, NS3, and NS5A. miR-185-5p was remarkably downregulated in HCV-infected primary hepatocytes and Huh7.5 cells. miR-185-5p upregulation inhibited HCV RNA expression, while its inhibition promoted HCV replication. miR-185-5p induced accumulation of NS3 and NS5A in the cells. Dual-luciferase reporter gene assay verified the targeted relationship between miR-185-5p and GALNT8. In addition, the effects of overexpressing or knocking down miR-185-5p on HCV replication could be correspondingly eliminated by the overexpression or knockdown of GALNT8. miR-185-5p may target GALNT8 in JFH1-infected Huh7.5 cells and then inhibit HCV replication. miR-185-5p may be a potential target for treating HCV. |
topic |
hepatitis c virus replication mir-185-5p galnt8 |
url |
https://doi.org/10.1515/biol-2021-0067 |
work_keys_str_mv |
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