Biological Control of Gom-chwi (Ligularia fischeri) Phytophthora Root Rot with Enterobacter asburiae ObRS-5 to Suppress Zoosporangia Formation and Zoospores Germination

Gom-chwi (Ligularia fischeri) is severely infected with Phytophthora drechsleri, the causal organism of Phytophthora root rot, an economically important crop disease that needs management throughout the cultivation period. In the present study, Phytophthora root rot was controlled by using bacterial...

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Main Authors: Dayeon Kim, Sang Yeob Lee, Seong Ho Ahn, Ji Hee Han, Jin Woo Park
Format: Article
Language:English
Published: Hanrimwon Publishing Company 2020-06-01
Series:The Plant Pathology Journal
Subjects:
Online Access:http://www.ppjonline.org/journal/view.html?doi=10.5423/PPJ.OA.11.2019.0283
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spelling doaj-a42f37a35b3f455ebb39a54eb69d1dcb2020-11-25T03:52:33ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542020-06-0136324425410.5423/PPJ.OA.11.2019.028310.5423PPJ.OA.11.2019.0283Biological Control of Gom-chwi (Ligularia fischeri) Phytophthora Root Rot with Enterobacter asburiae ObRS-5 to Suppress Zoosporangia Formation and Zoospores GerminationDayeon Kim0Sang Yeob Lee1Seong Ho Ahn2Ji Hee Han3Jin Woo Park4Division of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, KoreaDivision of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, KoreaDivision of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, KoreaDivision of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, KoreaDivision of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, KoreaGom-chwi (Ligularia fischeri) is severely infected with Phytophthora drechsleri, the causal organism of Phytophthora root rot, an economically important crop disease that needs management throughout the cultivation period. In the present study, Phytophthora root rot was controlled by using bacterial isolates from rhizosphere soils collected from various plants and screened for antagonistic activity against P. drechsleri. A total of 172 bacterial strains were isolated, of which, 49 strains showed antagonistic activities by dual culture assay. In the seedling assay, six out of the 49 strains showed a predominant effect on suppressing P. drechsleri. Among the six strains, the ObRS-5 strain showed remarkable against P. drechsleri when treated with seed dipping or soil drenching. The ObRS-5 strain was identified as Enterobacter asburiae based on 16S ribosomal RNA gene sequences analysis. The bacterial cells of E. asburiae ObRS-5 significantly suppressed sporangium formation and zoospore germination in P. drechsleri by 87.4% and 66.7%, respectively. In addition, culture filtrate of E. asburiae ObRS-5 also significantly inhibited sporangium formation and zoospore germination by 97.0% and 67.6%, respectively. Soil drenched bacterial cells, filtrate, and culture solution of E. asburiae ObRS-5 effectively suppressed Phytophthora root rot by 63.2%, 57.9%, and 81.1%, respectively. Thus, E. asburiae ObRS-5 could be used as a potential agent for the biological control of Phytophthora root rot infecting gom-chwi.http://www.ppjonline.org/journal/view.html?doi=10.5423/PPJ.OA.11.2019.0283antagonismbiological controlenterobacter asburiae obrs-5ligularia fischeriphytophthora drechsleri
collection DOAJ
language English
format Article
sources DOAJ
author Dayeon Kim
Sang Yeob Lee
Seong Ho Ahn
Ji Hee Han
Jin Woo Park
spellingShingle Dayeon Kim
Sang Yeob Lee
Seong Ho Ahn
Ji Hee Han
Jin Woo Park
Biological Control of Gom-chwi (Ligularia fischeri) Phytophthora Root Rot with Enterobacter asburiae ObRS-5 to Suppress Zoosporangia Formation and Zoospores Germination
The Plant Pathology Journal
antagonism
biological control
enterobacter asburiae obrs-5
ligularia fischeri
phytophthora drechsleri
author_facet Dayeon Kim
Sang Yeob Lee
Seong Ho Ahn
Ji Hee Han
Jin Woo Park
author_sort Dayeon Kim
title Biological Control of Gom-chwi (Ligularia fischeri) Phytophthora Root Rot with Enterobacter asburiae ObRS-5 to Suppress Zoosporangia Formation and Zoospores Germination
title_short Biological Control of Gom-chwi (Ligularia fischeri) Phytophthora Root Rot with Enterobacter asburiae ObRS-5 to Suppress Zoosporangia Formation and Zoospores Germination
title_full Biological Control of Gom-chwi (Ligularia fischeri) Phytophthora Root Rot with Enterobacter asburiae ObRS-5 to Suppress Zoosporangia Formation and Zoospores Germination
title_fullStr Biological Control of Gom-chwi (Ligularia fischeri) Phytophthora Root Rot with Enterobacter asburiae ObRS-5 to Suppress Zoosporangia Formation and Zoospores Germination
title_full_unstemmed Biological Control of Gom-chwi (Ligularia fischeri) Phytophthora Root Rot with Enterobacter asburiae ObRS-5 to Suppress Zoosporangia Formation and Zoospores Germination
title_sort biological control of gom-chwi (ligularia fischeri) phytophthora root rot with enterobacter asburiae obrs-5 to suppress zoosporangia formation and zoospores germination
publisher Hanrimwon Publishing Company
series The Plant Pathology Journal
issn 1598-2254
publishDate 2020-06-01
description Gom-chwi (Ligularia fischeri) is severely infected with Phytophthora drechsleri, the causal organism of Phytophthora root rot, an economically important crop disease that needs management throughout the cultivation period. In the present study, Phytophthora root rot was controlled by using bacterial isolates from rhizosphere soils collected from various plants and screened for antagonistic activity against P. drechsleri. A total of 172 bacterial strains were isolated, of which, 49 strains showed antagonistic activities by dual culture assay. In the seedling assay, six out of the 49 strains showed a predominant effect on suppressing P. drechsleri. Among the six strains, the ObRS-5 strain showed remarkable against P. drechsleri when treated with seed dipping or soil drenching. The ObRS-5 strain was identified as Enterobacter asburiae based on 16S ribosomal RNA gene sequences analysis. The bacterial cells of E. asburiae ObRS-5 significantly suppressed sporangium formation and zoospore germination in P. drechsleri by 87.4% and 66.7%, respectively. In addition, culture filtrate of E. asburiae ObRS-5 also significantly inhibited sporangium formation and zoospore germination by 97.0% and 67.6%, respectively. Soil drenched bacterial cells, filtrate, and culture solution of E. asburiae ObRS-5 effectively suppressed Phytophthora root rot by 63.2%, 57.9%, and 81.1%, respectively. Thus, E. asburiae ObRS-5 could be used as a potential agent for the biological control of Phytophthora root rot infecting gom-chwi.
topic antagonism
biological control
enterobacter asburiae obrs-5
ligularia fischeri
phytophthora drechsleri
url http://www.ppjonline.org/journal/view.html?doi=10.5423/PPJ.OA.11.2019.0283
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