Human Sperm Motility, Viability, and Morphology Decreased after Cryopreservation
The aim of this study was to analyze human sperm motility, viability, and morphology before and after cryopreservation. This true laboratory experimental study had pre and post randomized one group design. The study was conducted at the Embryology, Andrology, and Genetics Laboratory, Department of M...
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doaj-a43244f213f54cc194754aa1d5b6d5382021-08-03T04:14:26ZengUniversitas AirlanggaFolia Medica Indonesiana2355-83932599-056X2019-10-0155319820110.20473/fmi.v55i3.155017430Human Sperm Motility, Viability, and Morphology Decreased after CryopreservationNinik Darsini0Berliana Hamidah1Seso Sulijaya Suyono2Faisal Yusuf Ashari3R Haryanto Aswin4Rina Yudiwati5Department of Medical Biology, Faculty of Medicine, Universitas Airlangga, SurabayaDepartment of Medical Biology, Reproductive Health Master Program, Faculty of Medicine, Universitas Airlangga, SurabayaAndrology Program, Faculty of Medicine, Universitas Airlangga, Dr Soetomo Hospital, SurabayaDepartment of Medical Biology, Faculty of Medicine, Universitas Airlangga, SurabayaDepartment of Medical Biology, Faculty of Medicine, Universitas Airlangga, SurabayaDepartment of Medical Biology, Faculty of Medicine, Universitas Airlangga, SurabayaThe aim of this study was to analyze human sperm motility, viability, and morphology before and after cryopreservation. This true laboratory experimental study had pre and post randomized one group design. The study was conducted at the Embryology, Andrology, and Genetics Laboratory, Department of Medical Biology, Faculty of Medicine, Universitas Airlangga from August to November 2017. The eighteen samples of fresh semen were collected from male volunteers who agreed and signed the informed consent of the study. Samples were analyzed their motility, viability, and morphology before and after cryopreservation. Results of this study indicated differentiation between motility before and after cryopreservation. Cryopreservation process decreased progressive motility (42.22 + 9.46%; 17.83 + 6.24%; p< 0.0001) and increased the number of immotile spermatozoa (35.44 + 10.15%; 60.11 + 12.53%; p< 0.0001). Cryopreservation also decreased human sperm viability (73.78 + 8.91%; 40.83 + 12.89%; p< 0.0001) and morphology (10.94 + 4.96%; 7.39 + 3.90%; p< 0.0001). Cryopreservation of human spermatozoa caused the decreased of motility, viability, and morphology.https://e-journal.unair.ac.id/FMI/article/view/15501cryopreservationsperm morphologysperm motilitysperm viability |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ninik Darsini Berliana Hamidah Seso Sulijaya Suyono Faisal Yusuf Ashari R Haryanto Aswin Rina Yudiwati |
spellingShingle |
Ninik Darsini Berliana Hamidah Seso Sulijaya Suyono Faisal Yusuf Ashari R Haryanto Aswin Rina Yudiwati Human Sperm Motility, Viability, and Morphology Decreased after Cryopreservation Folia Medica Indonesiana cryopreservation sperm morphology sperm motility sperm viability |
author_facet |
Ninik Darsini Berliana Hamidah Seso Sulijaya Suyono Faisal Yusuf Ashari R Haryanto Aswin Rina Yudiwati |
author_sort |
Ninik Darsini |
title |
Human Sperm Motility, Viability, and Morphology Decreased after Cryopreservation |
title_short |
Human Sperm Motility, Viability, and Morphology Decreased after Cryopreservation |
title_full |
Human Sperm Motility, Viability, and Morphology Decreased after Cryopreservation |
title_fullStr |
Human Sperm Motility, Viability, and Morphology Decreased after Cryopreservation |
title_full_unstemmed |
Human Sperm Motility, Viability, and Morphology Decreased after Cryopreservation |
title_sort |
human sperm motility, viability, and morphology decreased after cryopreservation |
publisher |
Universitas Airlangga |
series |
Folia Medica Indonesiana |
issn |
2355-8393 2599-056X |
publishDate |
2019-10-01 |
description |
The aim of this study was to analyze human sperm motility, viability, and morphology before and after cryopreservation. This true laboratory experimental study had pre and post randomized one group design. The study was conducted at the Embryology, Andrology, and Genetics Laboratory, Department of Medical Biology, Faculty of Medicine, Universitas Airlangga from August to November 2017. The eighteen samples of fresh semen were collected from male volunteers who agreed and signed the informed consent of the study. Samples were analyzed their motility, viability, and morphology before and after cryopreservation. Results of this study indicated differentiation between motility before and after cryopreservation. Cryopreservation process decreased progressive motility (42.22 + 9.46%; 17.83 + 6.24%; p< 0.0001) and increased the number of immotile spermatozoa (35.44 + 10.15%; 60.11 + 12.53%; p< 0.0001). Cryopreservation also decreased human sperm viability (73.78 + 8.91%; 40.83 + 12.89%; p< 0.0001) and morphology (10.94 + 4.96%; 7.39 + 3.90%; p< 0.0001). Cryopreservation of human spermatozoa caused the decreased of motility, viability, and morphology. |
topic |
cryopreservation sperm morphology sperm motility sperm viability |
url |
https://e-journal.unair.ac.id/FMI/article/view/15501 |
work_keys_str_mv |
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