Expression and DNA Methylation Status of Long Non-coding RNA ZNF667-AS1 in Esophageal Squamous Cell Carcinoma Tissues
Objective To detect the expression and methylation status of long non-coding RNA ZNF667-AS1 in esophageal squamous cell carcinoma(ESCC) tissues. Methods qPCR and MSP Methods were respectively applied to detect the expression and methylation status of ZNF667-AS1 in esophageal cancer cell lines (Kyse1...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | zho |
Published: |
Magazine House of Cancer Research on Prevention and Treatment
2018-12-01
|
Series: | Zhongliu Fangzhi Yanjiu |
Subjects: | |
Online Access: | http://html.rhhz.net/ZLFZYJ/html/8578.2018.18.0586.htm |
id |
doaj-a44e9fc37daa4546b030f5867ea6dae8 |
---|---|
record_format |
Article |
spelling |
doaj-a44e9fc37daa4546b030f5867ea6dae82020-11-25T03:16:26ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85781000-85782018-12-01451297698010.3971/j.issn.1000-8578.2018.18.05868578.2018.18.0586Expression and DNA Methylation Status of Long Non-coding RNA ZNF667-AS1 in Esophageal Squamous Cell Carcinoma TissuesWU Xuan0KUANG Gang1REN Libing2GUO Wei3LIANG Jia4KANG Xiaoliang5GUO Yanli6DONG Zhiming7Laboratory of Pathology, Hebei Cancer Institute, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, ChinaLaboratory of Pathology, Hebei Cancer Institute, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, ChinaDepartment of Thoracic Surgery, Handan Central Hospital, Handan 056001, ChinaLaboratory of Pathology, Hebei Cancer Institute, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, ChinaLaboratory of Pathology, Hebei Cancer Institute, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, ChinaLaboratory of Pathology, Hebei Cancer Institute, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, ChinaLaboratory of Pathology, Hebei Cancer Institute, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, ChinaLaboratory of Pathology, Hebei Cancer Institute, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, ChinaObjective To detect the expression and methylation status of long non-coding RNA ZNF667-AS1 in esophageal squamous cell carcinoma(ESCC) tissues. Methods qPCR and MSP Methods were respectively applied to detect the expression and methylation status of ZNF667-AS1 in esophageal cancer cell lines (Kyse170, Eca109, TE1, TE13), ESCC and corresponding noncancerous tissues before or after DNA methyltransferase inhibitor 5-Aza-dC treatment. Results After treated with 5-Aza-dC, ZNF667-AS1 performed higher expression in the four esophageal cancer cell lines, and the methylation status of ZNF667-AS1 were decreased. The expression of ZNF667-AS1 in ESCC tissues was significantly lower than that in the corresponding normal tissues, and the methylation frequency of ZNF667-AS1 distal and exon1 regions CpG islands were all significantly different(P < 0.05). The methylation frequency of ZNF667-AS1 proximal promoter region CpG island in ESCC tissues was significantly higher than that in the corresponding normal tissues, and closely correlated with pathological differentiation and TNM stages; the methylation frequency in the low ZNF667-AS1 expression group was significantly higher than that in the high ZNF667-AS1 expression group. Conclusion The low expression of ZNF667-AS1 is closely related to the occurrence and development of ESCC, and its proximal promoter region methylation may be one of the mechanisms of ZNF667-AS1 silence.http://html.rhhz.net/ZLFZYJ/html/8578.2018.18.0586.htmesophageal squamous cell carcinomaznf667-as1long non-coding rnasdna methylation |
collection |
DOAJ |
language |
zho |
format |
Article |
sources |
DOAJ |
author |
WU Xuan KUANG Gang REN Libing GUO Wei LIANG Jia KANG Xiaoliang GUO Yanli DONG Zhiming |
spellingShingle |
WU Xuan KUANG Gang REN Libing GUO Wei LIANG Jia KANG Xiaoliang GUO Yanli DONG Zhiming Expression and DNA Methylation Status of Long Non-coding RNA ZNF667-AS1 in Esophageal Squamous Cell Carcinoma Tissues Zhongliu Fangzhi Yanjiu esophageal squamous cell carcinoma znf667-as1 long non-coding rnas dna methylation |
author_facet |
WU Xuan KUANG Gang REN Libing GUO Wei LIANG Jia KANG Xiaoliang GUO Yanli DONG Zhiming |
author_sort |
WU Xuan |
title |
Expression and DNA Methylation Status of Long Non-coding RNA ZNF667-AS1 in Esophageal Squamous Cell Carcinoma Tissues |
title_short |
Expression and DNA Methylation Status of Long Non-coding RNA ZNF667-AS1 in Esophageal Squamous Cell Carcinoma Tissues |
title_full |
Expression and DNA Methylation Status of Long Non-coding RNA ZNF667-AS1 in Esophageal Squamous Cell Carcinoma Tissues |
title_fullStr |
Expression and DNA Methylation Status of Long Non-coding RNA ZNF667-AS1 in Esophageal Squamous Cell Carcinoma Tissues |
title_full_unstemmed |
Expression and DNA Methylation Status of Long Non-coding RNA ZNF667-AS1 in Esophageal Squamous Cell Carcinoma Tissues |
title_sort |
expression and dna methylation status of long non-coding rna znf667-as1 in esophageal squamous cell carcinoma tissues |
publisher |
Magazine House of Cancer Research on Prevention and Treatment |
series |
Zhongliu Fangzhi Yanjiu |
issn |
1000-8578 1000-8578 |
publishDate |
2018-12-01 |
description |
Objective To detect the expression and methylation status of long non-coding RNA ZNF667-AS1 in esophageal squamous cell carcinoma(ESCC) tissues. Methods qPCR and MSP Methods were respectively applied to detect the expression and methylation status of ZNF667-AS1 in esophageal cancer cell lines (Kyse170, Eca109, TE1, TE13), ESCC and corresponding noncancerous tissues before or after DNA methyltransferase inhibitor 5-Aza-dC treatment. Results After treated with 5-Aza-dC, ZNF667-AS1 performed higher expression in the four esophageal cancer cell lines, and the methylation status of ZNF667-AS1 were decreased. The expression of ZNF667-AS1 in ESCC tissues was significantly lower than that in the corresponding normal tissues, and the methylation frequency of ZNF667-AS1 distal and exon1 regions CpG islands were all significantly different(P < 0.05). The methylation frequency of ZNF667-AS1 proximal promoter region CpG island in ESCC tissues was significantly higher than that in the corresponding normal tissues, and closely correlated with pathological differentiation and TNM stages; the methylation frequency in the low ZNF667-AS1 expression group was significantly higher than that in the high ZNF667-AS1 expression group. Conclusion The low expression of ZNF667-AS1 is closely related to the occurrence and development of ESCC, and its proximal promoter region methylation may be one of the mechanisms of ZNF667-AS1 silence. |
topic |
esophageal squamous cell carcinoma znf667-as1 long non-coding rnas dna methylation |
url |
http://html.rhhz.net/ZLFZYJ/html/8578.2018.18.0586.htm |
work_keys_str_mv |
AT wuxuan expressionanddnamethylationstatusoflongnoncodingrnaznf667as1inesophagealsquamouscellcarcinomatissues AT kuanggang expressionanddnamethylationstatusoflongnoncodingrnaznf667as1inesophagealsquamouscellcarcinomatissues AT renlibing expressionanddnamethylationstatusoflongnoncodingrnaznf667as1inesophagealsquamouscellcarcinomatissues AT guowei expressionanddnamethylationstatusoflongnoncodingrnaznf667as1inesophagealsquamouscellcarcinomatissues AT liangjia expressionanddnamethylationstatusoflongnoncodingrnaznf667as1inesophagealsquamouscellcarcinomatissues AT kangxiaoliang expressionanddnamethylationstatusoflongnoncodingrnaznf667as1inesophagealsquamouscellcarcinomatissues AT guoyanli expressionanddnamethylationstatusoflongnoncodingrnaznf667as1inesophagealsquamouscellcarcinomatissues AT dongzhiming expressionanddnamethylationstatusoflongnoncodingrnaznf667as1inesophagealsquamouscellcarcinomatissues |
_version_ |
1724636114294145024 |