Temporal Adaptive Changes in Contractility and Fatigability of Diaphragm Muscles from Streptozotocin-Diabetic Rats
Diabetes is characterized by ventilatory depression due to decreased diaphragm (DPH) function. This study investigated the changes in contractile properties of rat DPH muscles over a time interval encompassing from 4 days to 14 weeks after the onset of streptozotocin-induced diabetes, with and witho...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2010-01-01
|
Series: | Journal of Biomedicine and Biotechnology |
Online Access: | http://dx.doi.org/10.1155/2010/931903 |
id |
doaj-a4518c7dc4824e0aae5f57a8b42be3c3 |
---|---|
record_format |
Article |
spelling |
doaj-a4518c7dc4824e0aae5f57a8b42be3c32020-11-25T02:19:12ZengHindawi LimitedJournal of Biomedicine and Biotechnology1110-72431110-72512010-01-01201010.1155/2010/931903931903Temporal Adaptive Changes in Contractility and Fatigability of Diaphragm Muscles from Streptozotocin-Diabetic RatsMarco Brotto0Leticia Brotto1J.-P. Jin2Thomas M. Nosek3Andrea Romani4Muscle Biology Research Group (MUBIG), Schools of Nursing & Medicine, University of Missouri-Kansas City, Kansas City, MO 64108, USAMuscle Biology Research Group (MUBIG), Schools of Nursing & Medicine, University of Missouri-Kansas City, Kansas City, MO 64108, USADepartment of Physiology, School of Medicine, Wayne State University, Detroit, MI 48201, USADepartment of Physiology & Biophysics, School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USADepartment of Physiology & Biophysics, School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USADiabetes is characterized by ventilatory depression due to decreased diaphragm (DPH) function. This study investigated the changes in contractile properties of rat DPH muscles over a time interval encompassing from 4 days to 14 weeks after the onset of streptozotocin-induced diabetes, with and without insulin treatment for 2 weeks. Maximum tetanic force in intact DPH muscle strips and recovery from fatiguing stimulation were measured. An early (4-day) depression in contractile function in diabetic DPH was followed by gradual improvement in muscle function and fatigue recovery (8 weeks). DPH contractile function deteriorated again at 14 weeks, a process that was completely reversed by insulin treatment. Maximal contractile force and calcium sensitivity assessed in Triton-skinned DPH fibers showed a similar bimodal pattern and the same beneficial effect of insulin treatment. While an extensive analysis of the isoforms of the contractile and regulatory proteins was not conducted, Western blot analysis of tropomyosin suggests that the changes in diabetic DPH response depended, at least in part, on a switch in fiber type.http://dx.doi.org/10.1155/2010/931903 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marco Brotto Leticia Brotto J.-P. Jin Thomas M. Nosek Andrea Romani |
spellingShingle |
Marco Brotto Leticia Brotto J.-P. Jin Thomas M. Nosek Andrea Romani Temporal Adaptive Changes in Contractility and Fatigability of Diaphragm Muscles from Streptozotocin-Diabetic Rats Journal of Biomedicine and Biotechnology |
author_facet |
Marco Brotto Leticia Brotto J.-P. Jin Thomas M. Nosek Andrea Romani |
author_sort |
Marco Brotto |
title |
Temporal Adaptive Changes in Contractility and Fatigability of Diaphragm Muscles from Streptozotocin-Diabetic Rats |
title_short |
Temporal Adaptive Changes in Contractility and Fatigability of Diaphragm Muscles from Streptozotocin-Diabetic Rats |
title_full |
Temporal Adaptive Changes in Contractility and Fatigability of Diaphragm Muscles from Streptozotocin-Diabetic Rats |
title_fullStr |
Temporal Adaptive Changes in Contractility and Fatigability of Diaphragm Muscles from Streptozotocin-Diabetic Rats |
title_full_unstemmed |
Temporal Adaptive Changes in Contractility and Fatigability of Diaphragm Muscles from Streptozotocin-Diabetic Rats |
title_sort |
temporal adaptive changes in contractility and fatigability of diaphragm muscles from streptozotocin-diabetic rats |
publisher |
Hindawi Limited |
series |
Journal of Biomedicine and Biotechnology |
issn |
1110-7243 1110-7251 |
publishDate |
2010-01-01 |
description |
Diabetes is characterized by ventilatory depression due to decreased diaphragm (DPH) function. This study investigated the changes in contractile properties of rat DPH muscles over a time interval encompassing from 4 days to 14 weeks after the onset of streptozotocin-induced diabetes, with and without insulin treatment for 2 weeks. Maximum tetanic force in intact DPH muscle strips and recovery from fatiguing stimulation were measured. An early (4-day) depression in contractile function in diabetic DPH was followed by gradual improvement in muscle function and fatigue recovery (8 weeks). DPH contractile function deteriorated again at 14 weeks, a process that was completely reversed by insulin treatment. Maximal contractile force and calcium sensitivity assessed in Triton-skinned DPH fibers showed a similar bimodal pattern and the same beneficial effect of insulin treatment. While an extensive analysis of the isoforms of the contractile and regulatory proteins was not conducted, Western blot analysis of tropomyosin suggests that the changes in diabetic DPH response depended, at least in part, on a switch in fiber type. |
url |
http://dx.doi.org/10.1155/2010/931903 |
work_keys_str_mv |
AT marcobrotto temporaladaptivechangesincontractilityandfatigabilityofdiaphragmmusclesfromstreptozotocindiabeticrats AT leticiabrotto temporaladaptivechangesincontractilityandfatigabilityofdiaphragmmusclesfromstreptozotocindiabeticrats AT jpjin temporaladaptivechangesincontractilityandfatigabilityofdiaphragmmusclesfromstreptozotocindiabeticrats AT thomasmnosek temporaladaptivechangesincontractilityandfatigabilityofdiaphragmmusclesfromstreptozotocindiabeticrats AT andrearomani temporaladaptivechangesincontractilityandfatigabilityofdiaphragmmusclesfromstreptozotocindiabeticrats |
_version_ |
1724877763685384192 |