Polymeric nanoparticles containing Tithonia diversifolia (hemsl) a. Gray flowers alcoholic extract and coated by hyaluronic acid_ development, synthesis, and characterization

• Main Authors: Fernanda Arriel Pedrozo Rezende, Anna Luiza de Aveiro Ruocco, Bruno Batista da Silva, Robson Miranda da Gama, José Armando Jr, Marcelo Guimarães
• Format: Article
• Language: English
• Published: Instituto de Osmologia e Óleos Essencias 2020-03-01
• Series: Brazilian Journal of Natural Sciences
• Subjects: Polymeric nanoparticles; Tithonia diversifolia (Hemsl) A. Gray; poly (n-butyl-cyanoacrylate), hyaluronic acid.
• Online Access: https://bjns.com.br/index.php/BJNS/article/view/88

Nanoparticles are systems formed by biodegradable, synthetic or natural polymers, that have gained significant importance in different industry segments, as well as being the highlight of essential researches. These tiny particles present various promising characteristics, such as: site-specific and gradual drug release and improvement of active principle solubility and stability. The combination of nanotechnology with medicinal plants could be able to enhance the action of plant extracts, such so that many innovative drug carriers have emerged, including polymeric nanoparticles. The objective of this study was to synthesize and characterize polymeric nanoparticles coated with hyaluronic acid, containing Tithonia diversifolia (Hemsl) A. Gray flowers alcoholic extract, aiming to improve the nanoparticle’s stability, for possible future applications. The synthesis occurred through emulsion polymerization, where the monomer n-butyl-cyanoacrylate was incorporated with Dextran® into an aqueous HCl solution, with posterior addition of the alcoholic plant extract and neutralization reaction with NaOH. Stability parameters, such as hydrodynamic diameter, polydispersity index, zeta potential, spectroscopy (IV), Scanning Electron Microscopy (SEM), thermogravimetry (TG) and Differential Exploration Calorimetry (DSC) were analysed. The nanoparticle system was evaluated taking into consideration particle stability prior and posterior to the addition of the extract, as well as coated and uncoated particles. The results demonstrated the good reactivity of the monomers of cyanoacrylates, as well as effectiveness of hyaluronic acid in relation to the proposed objective, evidenced in the results obtained by Infrared Spectroscopy, by SEM, DSC and, also, TG. The study demonstrated that there are possible future applications for this method.