Tracking of the biochemical changes upon pleomorphic adenoma progression using vibrational microspectroscopy
Abstract Head and neck tumors can be very challenging to treat because of the risk of problems or complications after surgery. Therefore, prompt and accurate diagnosis is extremely important to drive appropriate treatment decisions, which may reduce the chance of recurrence. This paper presents the...
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doaj-a486b7d9013d4d40aecc56d234b70c8e2021-09-12T11:24:53ZengNature Publishing GroupScientific Reports2045-23222021-09-0111111110.1038/s41598-021-97377-2Tracking of the biochemical changes upon pleomorphic adenoma progression using vibrational microspectroscopyCzesława Paluszkiewicz0Maciej Roman1Natalia Piergies2Ewa Pięta3Monika Woźniak4Mariangela Cestelli Guidi5Katarzyna Miśkiewicz-Orczyk6Magdalena Marków7Wojciech Ścierski8Maciej Misiołek9Bogna Drozdzowska10Wojciech M. Kwiatek11Institute of Nuclear Physics, Polish Academy of SciencesInstitute of Nuclear Physics, Polish Academy of SciencesInstitute of Nuclear Physics, Polish Academy of SciencesInstitute of Nuclear Physics, Polish Academy of SciencesInstitute of Nuclear Physics, Polish Academy of SciencesINFN-Laboratori Nazionali di FrascatiDepartment of Otorhinolaryngology and Laryngological Oncology in Zabrze, Medical University of Silesia KatowiceDepartment of Otorhinolaryngology and Laryngological Oncology in Zabrze, Medical University of Silesia KatowiceDepartment of Otorhinolaryngology and Laryngological Oncology in Zabrze, Medical University of Silesia KatowiceDepartment of Otorhinolaryngology and Laryngological Oncology in Zabrze, Medical University of Silesia KatowiceDepartment of Pathomorphology Zabrze, Medical University of SilesiaInstitute of Nuclear Physics, Polish Academy of SciencesAbstract Head and neck tumors can be very challenging to treat because of the risk of problems or complications after surgery. Therefore, prompt and accurate diagnosis is extremely important to drive appropriate treatment decisions, which may reduce the chance of recurrence. This paper presents the original research exploring the feasibility of Fourier transform infrared (FT-IR) and Raman spectroscopy (RS) methods to investigate biochemical alterations upon the development of the pleomorphic adenoma. Principal component analysis (PCA) was used for a detailed assessment of the observed changes and to determine the spectroscopic basis for salivary gland neoplastic pathogenesis. It is implied that within the healthy margin, as opposed to the tumoral tissue, there are parts that differ significantly in lipid content. This observation shed new light on the crucial role of lipids in tissue physiology and tumorigenesis. Thus, a novel approach that eliminates the influence of lipids on the elucidation of biochemical changes is proposed. The performed analysis suggests that the highly heterogeneous healthy margin contains more unsaturated triacylglycerols, while the tumoral section is rich in proteins. The difference in protein content was also observed for these two tissue types, i.e. the healthy tissue possesses more proteins in the anti-parallel β-sheet conformation, whereas the tumoral tissue is dominated by proteins rich in unordered random coils. Furthermore, the pathogenic tissue shows a higher content of carbohydrates and reveals noticeable differences in nucleic acid content. Finally, FT-IR and Raman spectroscopy methods were proposed as very promising methods in the discrimination of tumoral and healthy tissues of the salivary gland.https://doi.org/10.1038/s41598-021-97377-2 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Czesława Paluszkiewicz Maciej Roman Natalia Piergies Ewa Pięta Monika Woźniak Mariangela Cestelli Guidi Katarzyna Miśkiewicz-Orczyk Magdalena Marków Wojciech Ścierski Maciej Misiołek Bogna Drozdzowska Wojciech M. Kwiatek |
spellingShingle |
Czesława Paluszkiewicz Maciej Roman Natalia Piergies Ewa Pięta Monika Woźniak Mariangela Cestelli Guidi Katarzyna Miśkiewicz-Orczyk Magdalena Marków Wojciech Ścierski Maciej Misiołek Bogna Drozdzowska Wojciech M. Kwiatek Tracking of the biochemical changes upon pleomorphic adenoma progression using vibrational microspectroscopy Scientific Reports |
author_facet |
Czesława Paluszkiewicz Maciej Roman Natalia Piergies Ewa Pięta Monika Woźniak Mariangela Cestelli Guidi Katarzyna Miśkiewicz-Orczyk Magdalena Marków Wojciech Ścierski Maciej Misiołek Bogna Drozdzowska Wojciech M. Kwiatek |
author_sort |
Czesława Paluszkiewicz |
title |
Tracking of the biochemical changes upon pleomorphic adenoma progression using vibrational microspectroscopy |
title_short |
Tracking of the biochemical changes upon pleomorphic adenoma progression using vibrational microspectroscopy |
title_full |
Tracking of the biochemical changes upon pleomorphic adenoma progression using vibrational microspectroscopy |
title_fullStr |
Tracking of the biochemical changes upon pleomorphic adenoma progression using vibrational microspectroscopy |
title_full_unstemmed |
Tracking of the biochemical changes upon pleomorphic adenoma progression using vibrational microspectroscopy |
title_sort |
tracking of the biochemical changes upon pleomorphic adenoma progression using vibrational microspectroscopy |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-09-01 |
description |
Abstract Head and neck tumors can be very challenging to treat because of the risk of problems or complications after surgery. Therefore, prompt and accurate diagnosis is extremely important to drive appropriate treatment decisions, which may reduce the chance of recurrence. This paper presents the original research exploring the feasibility of Fourier transform infrared (FT-IR) and Raman spectroscopy (RS) methods to investigate biochemical alterations upon the development of the pleomorphic adenoma. Principal component analysis (PCA) was used for a detailed assessment of the observed changes and to determine the spectroscopic basis for salivary gland neoplastic pathogenesis. It is implied that within the healthy margin, as opposed to the tumoral tissue, there are parts that differ significantly in lipid content. This observation shed new light on the crucial role of lipids in tissue physiology and tumorigenesis. Thus, a novel approach that eliminates the influence of lipids on the elucidation of biochemical changes is proposed. The performed analysis suggests that the highly heterogeneous healthy margin contains more unsaturated triacylglycerols, while the tumoral section is rich in proteins. The difference in protein content was also observed for these two tissue types, i.e. the healthy tissue possesses more proteins in the anti-parallel β-sheet conformation, whereas the tumoral tissue is dominated by proteins rich in unordered random coils. Furthermore, the pathogenic tissue shows a higher content of carbohydrates and reveals noticeable differences in nucleic acid content. Finally, FT-IR and Raman spectroscopy methods were proposed as very promising methods in the discrimination of tumoral and healthy tissues of the salivary gland. |
url |
https://doi.org/10.1038/s41598-021-97377-2 |
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