Cold Plasma Affects Germination and Fungal Community Structure of Buckwheat Seeds
Crop seeds are frequently colonised by fungi from the field or storage places. Some fungi can cause plant diseases or produce mycotoxins, compromising the use of seeds as seeding material, food or feed. We have investigated the effects of cold plasma (CP) on seed germination and diversity of seed-bo...
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doaj-a4a208c1e9a64f6aaf29d940fedfce5d2021-04-23T23:01:44ZengMDPI AGPlants2223-77472021-04-011085185110.3390/plants10050851Cold Plasma Affects Germination and Fungal Community Structure of Buckwheat SeedsJure Mravlje0Marjana Regvar1Pia Starič2Miran Mozetič3Katarina Vogel-Mikuš4Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, SloveniaBiotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, SloveniaJozef Stefan Institute, Jamova 39, 1000 Ljubljana, SloveniaJozef Stefan Institute, Jamova 39, 1000 Ljubljana, SloveniaBiotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, SloveniaCrop seeds are frequently colonised by fungi from the field or storage places. Some fungi can cause plant diseases or produce mycotoxins, compromising the use of seeds as seeding material, food or feed. We have investigated the effects of cold plasma (CP) on seed germination and diversity of seed-borne fungi in common and Tartary buckwheat. The seeds were treated with CP for 15, 30, 45, 60, 90, and 120 s in a low-pressure radiofrequency system using oxygen as the feed gas. The fungi from the seed surface and fungal endophytes were isolated using potato dextrose agar plates. After identification by molecular methods, the frequency and diversity of fungal strains were compared between CP treated and chemically surface-sterilised (30% of H<sub>2</sub>O<sub>2</sub>) seeds. CP treatments above 60 s negatively affected the germination of both buckwheat species. A significant reduction in fungal frequency and diversity was observed after 90 s and 120 s in common and Tartary buckwheat, respectively. The filamentous fungi of genera <i>Alternaria</i> and <i>Epicoccum</i> proved to be the most resistant to CP. The results of our study indicate that CP treatment used in our study may be applicable in postharvest and food production, but not for further seed sowing.https://www.mdpi.com/2223-7747/10/5/851cold plasmaseedsgrainsplantscommon buckwheatTartary buckwheat |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jure Mravlje Marjana Regvar Pia Starič Miran Mozetič Katarina Vogel-Mikuš |
spellingShingle |
Jure Mravlje Marjana Regvar Pia Starič Miran Mozetič Katarina Vogel-Mikuš Cold Plasma Affects Germination and Fungal Community Structure of Buckwheat Seeds Plants cold plasma seeds grains plants common buckwheat Tartary buckwheat |
author_facet |
Jure Mravlje Marjana Regvar Pia Starič Miran Mozetič Katarina Vogel-Mikuš |
author_sort |
Jure Mravlje |
title |
Cold Plasma Affects Germination and Fungal Community Structure of Buckwheat Seeds |
title_short |
Cold Plasma Affects Germination and Fungal Community Structure of Buckwheat Seeds |
title_full |
Cold Plasma Affects Germination and Fungal Community Structure of Buckwheat Seeds |
title_fullStr |
Cold Plasma Affects Germination and Fungal Community Structure of Buckwheat Seeds |
title_full_unstemmed |
Cold Plasma Affects Germination and Fungal Community Structure of Buckwheat Seeds |
title_sort |
cold plasma affects germination and fungal community structure of buckwheat seeds |
publisher |
MDPI AG |
series |
Plants |
issn |
2223-7747 |
publishDate |
2021-04-01 |
description |
Crop seeds are frequently colonised by fungi from the field or storage places. Some fungi can cause plant diseases or produce mycotoxins, compromising the use of seeds as seeding material, food or feed. We have investigated the effects of cold plasma (CP) on seed germination and diversity of seed-borne fungi in common and Tartary buckwheat. The seeds were treated with CP for 15, 30, 45, 60, 90, and 120 s in a low-pressure radiofrequency system using oxygen as the feed gas. The fungi from the seed surface and fungal endophytes were isolated using potato dextrose agar plates. After identification by molecular methods, the frequency and diversity of fungal strains were compared between CP treated and chemically surface-sterilised (30% of H<sub>2</sub>O<sub>2</sub>) seeds. CP treatments above 60 s negatively affected the germination of both buckwheat species. A significant reduction in fungal frequency and diversity was observed after 90 s and 120 s in common and Tartary buckwheat, respectively. The filamentous fungi of genera <i>Alternaria</i> and <i>Epicoccum</i> proved to be the most resistant to CP. The results of our study indicate that CP treatment used in our study may be applicable in postharvest and food production, but not for further seed sowing. |
topic |
cold plasma seeds grains plants common buckwheat Tartary buckwheat |
url |
https://www.mdpi.com/2223-7747/10/5/851 |
work_keys_str_mv |
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