<b>Molecular heterogeneity of <em>Malassezia pachydermatis </em>through RAPD-PCR</b> - doi: 10.4025/actascibiolsci.v32i2.4788 <b>Molecular heterogeneity of <em>Malassezia pachydermatis </em>through RAPD-PCR</b> - doi: 10.4025/actascibiolsci.v32i2.4788

• Main Authors: João Roberto Braga Mello, Luiz Filipe Damé Schuch, Mário Carlos Araújo Meireles, Ana Raquel Mano Meinerz, Helen Silveira Coimbra, Patrícia da Silva Nascente
• Format: Article
• Language: English
• Published: Universidade Estadual de Maringá 2010-05-01
• Series: Acta Scientiarum : Biological Sciences
• Subjects: Malassezia pachydermatis; molecular biology; otitis externa; dogs
• Online Access: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/4788

Several methodologies in molecular biology have been used in the investigation of <em>Malassezia pachydermatis</em> and its differentiation into subtypes. Recent molecular research of this species includes the use of samples isolated from canine otitis externa and dermatitis, as well as from healthy animals, having in view an epidemiological study of the yeast. The aim of this study was to identify molecular differences in <em>M. pachydermatis</em> samples isolated from dogs with otitis externa. The <em>M. pachydermatis</em> strains were analyzed by means of the Random Amplification Primer DNA - Polimerase Chain Reaction (RAPD–PCR) for molecular heterogeneity research. DNA extraction was carried out with phenol-chloroform and the RAPD technique using the AGAATCCGCC primer. A variation was observed in the number and arrangement of the bands among the 49 studied isolates, grouped into nine patterns. Isolate groupings were not found to be related to animal breed, age or sex. It was concluded that M. <em>pachydermatis</em> has differences in its molecular profile, as shown by the molecular technique (RAPD – PCR), which allows isolates to be classified into nine subtypes.<br>Several methodologies in molecular biology have been used in the investigation of <em>Malassezia pachydermatis</em> and its differentiation into subtypes. Recent molecular research of this species includes the use of samples isolated from canine otitis externa and dermatitis, as well as from healthy animals, having in view an epidemiological study of the yeast. The aim of this study was to identify molecular differences in <em>M. pachydermatis</em> samples isolated from dogs with otitis externa. The <em>M. pachydermatis</em> strains were analyzed by means of the Random Amplification Primer DNA - Polimerase Chain Reaction (RAPD–PCR) for molecular heterogeneity research. DNA extraction was carried out with phenol-chloroform and the RAPD technique using the AGAATCCGCC primer. A variation was observed in the number and arrangement of the bands among the 49 studied isolates, grouped into nine patterns. Isolate groupings were not found to be related to animal breed, age or sex. It was concluded that M. <em>pachydermatis</em> has differences in its molecular profile, as shown by the molecular technique (RAPD – PCR), which allows isolates to be classified into nine subtypes.