Diagnosing and mapping pulmonary emphysema on X-ray projection images: incremental value of grating-based X-ray dark-field imaging.
PURPOSE: To assess whether grating-based X-ray dark-field imaging can increase the sensitivity of X-ray projection images in the diagnosis of pulmonary emphysema and allow for a more accurate assessment of emphysema distribution. MATERIALS AND METHODS: Lungs from three mice with pulmonary emphysema...
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doaj-a4c4cae8b2eb45919c9194d6eaa35d342020-11-25T00:53:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0183e5952610.1371/journal.pone.0059526Diagnosing and mapping pulmonary emphysema on X-ray projection images: incremental value of grating-based X-ray dark-field imaging.Felix G MeinelFelix SchwabSimone SchleedeMartin BechJulia HerzenKlaus AchterholdSigrid AuweterFabian BambergAli Ö YildirimAlexander BohlaOliver EickelbergRod LoewenMartin GiffordRonald RuthMaximilian F ReiserFranz PfeifferKonstantin NikolaouPURPOSE: To assess whether grating-based X-ray dark-field imaging can increase the sensitivity of X-ray projection images in the diagnosis of pulmonary emphysema and allow for a more accurate assessment of emphysema distribution. MATERIALS AND METHODS: Lungs from three mice with pulmonary emphysema and three healthy mice were imaged ex vivo using a laser-driven compact synchrotron X-ray source. Median signal intensities of transmission (T), dark-field (V) and a combined parameter (normalized scatter) were compared between emphysema and control group. To determine the diagnostic value of each parameter in differentiating between healthy and emphysematous lung tissue, a receiver-operating-characteristic (ROC) curve analysis was performed both on a per-pixel and a per-individual basis. Parametric maps of emphysema distribution were generated using transmission, dark-field and normalized scatter signal and correlated with histopathology. RESULTS: Transmission values relative to water were higher for emphysematous lungs than for control lungs (1.11 vs. 1.06, p<0.001). There was no difference in median dark-field signal intensities between both groups (0.66 vs. 0.66). Median normalized scatter was significantly lower in the emphysematous lungs compared to controls (4.9 vs. 10.8, p<0.001), and was the best parameter for differentiation of healthy vs. emphysematous lung tissue. In a per-pixel analysis, the area under the ROC curve (AUC) for the normalized scatter value was significantly higher than for transmission (0.86 vs. 0.78, p<0.001) and dark-field value (0.86 vs. 0.52, p<0.001) alone. Normalized scatter showed very high sensitivity for a wide range of specificity values (94% sensitivity at 75% specificity). Using the normalized scatter signal to display the regional distribution of emphysema provides color-coded parametric maps, which show the best correlation with histopathology. CONCLUSION: In a murine model, the complementary information provided by X-ray transmission and dark-field images adds incremental diagnostic value in detecting pulmonary emphysema and visualizing its regional distribution as compared to conventional X-ray projections.http://europepmc.org/articles/PMC3608711?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Felix G Meinel Felix Schwab Simone Schleede Martin Bech Julia Herzen Klaus Achterhold Sigrid Auweter Fabian Bamberg Ali Ö Yildirim Alexander Bohla Oliver Eickelberg Rod Loewen Martin Gifford Ronald Ruth Maximilian F Reiser Franz Pfeiffer Konstantin Nikolaou |
spellingShingle |
Felix G Meinel Felix Schwab Simone Schleede Martin Bech Julia Herzen Klaus Achterhold Sigrid Auweter Fabian Bamberg Ali Ö Yildirim Alexander Bohla Oliver Eickelberg Rod Loewen Martin Gifford Ronald Ruth Maximilian F Reiser Franz Pfeiffer Konstantin Nikolaou Diagnosing and mapping pulmonary emphysema on X-ray projection images: incremental value of grating-based X-ray dark-field imaging. PLoS ONE |
author_facet |
Felix G Meinel Felix Schwab Simone Schleede Martin Bech Julia Herzen Klaus Achterhold Sigrid Auweter Fabian Bamberg Ali Ö Yildirim Alexander Bohla Oliver Eickelberg Rod Loewen Martin Gifford Ronald Ruth Maximilian F Reiser Franz Pfeiffer Konstantin Nikolaou |
author_sort |
Felix G Meinel |
title |
Diagnosing and mapping pulmonary emphysema on X-ray projection images: incremental value of grating-based X-ray dark-field imaging. |
title_short |
Diagnosing and mapping pulmonary emphysema on X-ray projection images: incremental value of grating-based X-ray dark-field imaging. |
title_full |
Diagnosing and mapping pulmonary emphysema on X-ray projection images: incremental value of grating-based X-ray dark-field imaging. |
title_fullStr |
Diagnosing and mapping pulmonary emphysema on X-ray projection images: incremental value of grating-based X-ray dark-field imaging. |
title_full_unstemmed |
Diagnosing and mapping pulmonary emphysema on X-ray projection images: incremental value of grating-based X-ray dark-field imaging. |
title_sort |
diagnosing and mapping pulmonary emphysema on x-ray projection images: incremental value of grating-based x-ray dark-field imaging. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
PURPOSE: To assess whether grating-based X-ray dark-field imaging can increase the sensitivity of X-ray projection images in the diagnosis of pulmonary emphysema and allow for a more accurate assessment of emphysema distribution. MATERIALS AND METHODS: Lungs from three mice with pulmonary emphysema and three healthy mice were imaged ex vivo using a laser-driven compact synchrotron X-ray source. Median signal intensities of transmission (T), dark-field (V) and a combined parameter (normalized scatter) were compared between emphysema and control group. To determine the diagnostic value of each parameter in differentiating between healthy and emphysematous lung tissue, a receiver-operating-characteristic (ROC) curve analysis was performed both on a per-pixel and a per-individual basis. Parametric maps of emphysema distribution were generated using transmission, dark-field and normalized scatter signal and correlated with histopathology. RESULTS: Transmission values relative to water were higher for emphysematous lungs than for control lungs (1.11 vs. 1.06, p<0.001). There was no difference in median dark-field signal intensities between both groups (0.66 vs. 0.66). Median normalized scatter was significantly lower in the emphysematous lungs compared to controls (4.9 vs. 10.8, p<0.001), and was the best parameter for differentiation of healthy vs. emphysematous lung tissue. In a per-pixel analysis, the area under the ROC curve (AUC) for the normalized scatter value was significantly higher than for transmission (0.86 vs. 0.78, p<0.001) and dark-field value (0.86 vs. 0.52, p<0.001) alone. Normalized scatter showed very high sensitivity for a wide range of specificity values (94% sensitivity at 75% specificity). Using the normalized scatter signal to display the regional distribution of emphysema provides color-coded parametric maps, which show the best correlation with histopathology. CONCLUSION: In a murine model, the complementary information provided by X-ray transmission and dark-field images adds incremental diagnostic value in detecting pulmonary emphysema and visualizing its regional distribution as compared to conventional X-ray projections. |
url |
http://europepmc.org/articles/PMC3608711?pdf=render |
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