Half-life modeling of basic fibroblast growth factor released from growth factor-eluting polyelectrolyte multilayers
Abstract Growth factor-eluting polymer systems have been widely reported to improve cell and tissue outcomes; however, measurements of actual growth factor concentration in cell culture conditions are limited. The problem is compounded by a lack of knowledge of growth factor half-lives, which impede...
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2021-05-01
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Online Access: | https://doi.org/10.1038/s41598-021-89229-w |
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doaj-a4d30666bc5a4ff6be41e1846f35baad2021-05-09T11:31:59ZengNature Publishing GroupScientific Reports2045-23222021-05-0111111310.1038/s41598-021-89229-wHalf-life modeling of basic fibroblast growth factor released from growth factor-eluting polyelectrolyte multilayersIvan Ding0Amy M. Peterson1Department of Chemical Engineering, University of Massachusetts LowellDepartment of Plastics Engineering, University of Massachusetts LowellAbstract Growth factor-eluting polymer systems have been widely reported to improve cell and tissue outcomes; however, measurements of actual growth factor concentration in cell culture conditions are limited. The problem is compounded by a lack of knowledge of growth factor half-lives, which impedes efforts to determine real-time growth factor concentrations. In this work, the half-life of basic fibroblast growth factor (FGF2) was determined using enzyme linked immunosorbent assay (ELISA). FGF2 release from polyelectrolyte multilayers (PEMs) was measured and the data was fit to a simple degradation model, allowing for the determination of FGF2 concentrations between 2 and 4 days of culture time. After the first hour, the FGF2 concentration for PEMs assembled at pH = 4 ranged from 2.67 ng/mL to 5.76 ng/mL, while for PEMs assembled at pH = 5, the concentration ranged from 0.62 ng/mL to 2.12 ng/mL. CRL-2352 fibroblasts were cultured on PEMs assembled at pH = 4 and pH = 5. After 2 days, the FGF2-eluting PEM conditions showed improved cell count and spreading. After 4 days, only the pH = 4 assembly condition had higher cells counts, while the PEM assembled at pH = 5 and PEM with no FGF2 showed increased spreading. Overall, the half-life model and cell culture study provide optimal concentration ranges for fibroblast proliferation and a framework for understanding how temporal FGF2 concentration may affect other cell types.https://doi.org/10.1038/s41598-021-89229-w |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ivan Ding Amy M. Peterson |
spellingShingle |
Ivan Ding Amy M. Peterson Half-life modeling of basic fibroblast growth factor released from growth factor-eluting polyelectrolyte multilayers Scientific Reports |
author_facet |
Ivan Ding Amy M. Peterson |
author_sort |
Ivan Ding |
title |
Half-life modeling of basic fibroblast growth factor released from growth factor-eluting polyelectrolyte multilayers |
title_short |
Half-life modeling of basic fibroblast growth factor released from growth factor-eluting polyelectrolyte multilayers |
title_full |
Half-life modeling of basic fibroblast growth factor released from growth factor-eluting polyelectrolyte multilayers |
title_fullStr |
Half-life modeling of basic fibroblast growth factor released from growth factor-eluting polyelectrolyte multilayers |
title_full_unstemmed |
Half-life modeling of basic fibroblast growth factor released from growth factor-eluting polyelectrolyte multilayers |
title_sort |
half-life modeling of basic fibroblast growth factor released from growth factor-eluting polyelectrolyte multilayers |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-05-01 |
description |
Abstract Growth factor-eluting polymer systems have been widely reported to improve cell and tissue outcomes; however, measurements of actual growth factor concentration in cell culture conditions are limited. The problem is compounded by a lack of knowledge of growth factor half-lives, which impedes efforts to determine real-time growth factor concentrations. In this work, the half-life of basic fibroblast growth factor (FGF2) was determined using enzyme linked immunosorbent assay (ELISA). FGF2 release from polyelectrolyte multilayers (PEMs) was measured and the data was fit to a simple degradation model, allowing for the determination of FGF2 concentrations between 2 and 4 days of culture time. After the first hour, the FGF2 concentration for PEMs assembled at pH = 4 ranged from 2.67 ng/mL to 5.76 ng/mL, while for PEMs assembled at pH = 5, the concentration ranged from 0.62 ng/mL to 2.12 ng/mL. CRL-2352 fibroblasts were cultured on PEMs assembled at pH = 4 and pH = 5. After 2 days, the FGF2-eluting PEM conditions showed improved cell count and spreading. After 4 days, only the pH = 4 assembly condition had higher cells counts, while the PEM assembled at pH = 5 and PEM with no FGF2 showed increased spreading. Overall, the half-life model and cell culture study provide optimal concentration ranges for fibroblast proliferation and a framework for understanding how temporal FGF2 concentration may affect other cell types. |
url |
https://doi.org/10.1038/s41598-021-89229-w |
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