β-Peltoboykinolic Acid from <i>Astilbe rubra</i> Attenuates TGF-β1-Induced Epithelial-to-Mesenchymal Transitions in Lung Alveolar Epithelial Cells
Epithelial-to-mesenchymal transition (EMT) is increasingly recognized as contributing to the pathogenesis of idiopathic pulmonary fibrosis. Therefore, novel plant-based natural, active compounds have been sought for the treatment of fibrotic EMT. The aim of the present study was to investigate the i...
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doaj-a4eb3cccb0aa4890adcf0086604686722020-11-25T01:50:37ZengMDPI AGMolecules1420-30492019-07-012414257310.3390/molecules24142573molecules24142573β-Peltoboykinolic Acid from <i>Astilbe rubra</i> Attenuates TGF-β1-Induced Epithelial-to-Mesenchymal Transitions in Lung Alveolar Epithelial CellsIn Jae Bang0Ha Ryong Kim1Yukyoung Jeon2Mi Ho Jeong3Yong Joo Park4Jong Hwan Kwak5Kyu Hyuck Chung6School of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaCollege of Pharmacy, Daegu Catholic University, Gyeongsan 38430, KoreaSchool of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaSchool of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaSchool of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaSchool of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaSchool of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaEpithelial-to-mesenchymal transition (EMT) is increasingly recognized as contributing to the pathogenesis of idiopathic pulmonary fibrosis. Therefore, novel plant-based natural, active compounds have been sought for the treatment of fibrotic EMT. The aim of the present study was to investigate the inhibitory effects of <i>Astilbe rubra</i> on TGF-β1-induced EMT in lung alveolar epithelial cells (A549). <i>A. rubra</i> was subjected to extraction using 70% ethanol (ARE), and ethanol extracts of the aerial part and that of the rhizome were further partitioned using various solvents. Protein expression and cell motility were investigated to evaluate the inhibitory effects of ARE on EMT. EMT occurred in A549 cells treated with TGF-β1, but was prevented by co-treatment with ARE. The dichloromethane fractions showed the strongest inhibitory effect on TGF-β1-induced EMT. β-Peltoboykinolic acid was isolated from the dichloromethane fractions of <i>A. rubra</i> by activity-oriented isolation. β-Peltoboykinolic acid not only attenuated TGF-β1-induced EMT, but also the overproduction of extracellular matrix components including type I collagen and fibronectin. The Smad pathway activated by TGF-β1 was inhibited by co-treatment with β-peltoboykinolic acid. Taken together, these results indicate that β-peltoboykinolic acid from <i>A. rubra</i> and dichloromethane fractions shows potential as an antifibrotic agent in A549 cells treated with TGF-β1.https://www.mdpi.com/1420-3049/24/14/2573<i>Astilbe rubra</i>β-peltoboykinolic acidepithelial-mesenchymal transitionTGF-β1lung fibrosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
In Jae Bang Ha Ryong Kim Yukyoung Jeon Mi Ho Jeong Yong Joo Park Jong Hwan Kwak Kyu Hyuck Chung |
spellingShingle |
In Jae Bang Ha Ryong Kim Yukyoung Jeon Mi Ho Jeong Yong Joo Park Jong Hwan Kwak Kyu Hyuck Chung β-Peltoboykinolic Acid from <i>Astilbe rubra</i> Attenuates TGF-β1-Induced Epithelial-to-Mesenchymal Transitions in Lung Alveolar Epithelial Cells Molecules <i>Astilbe rubra</i> β-peltoboykinolic acid epithelial-mesenchymal transition TGF-β1 lung fibrosis |
author_facet |
In Jae Bang Ha Ryong Kim Yukyoung Jeon Mi Ho Jeong Yong Joo Park Jong Hwan Kwak Kyu Hyuck Chung |
author_sort |
In Jae Bang |
title |
β-Peltoboykinolic Acid from <i>Astilbe rubra</i> Attenuates TGF-β1-Induced Epithelial-to-Mesenchymal Transitions in Lung Alveolar Epithelial Cells |
title_short |
β-Peltoboykinolic Acid from <i>Astilbe rubra</i> Attenuates TGF-β1-Induced Epithelial-to-Mesenchymal Transitions in Lung Alveolar Epithelial Cells |
title_full |
β-Peltoboykinolic Acid from <i>Astilbe rubra</i> Attenuates TGF-β1-Induced Epithelial-to-Mesenchymal Transitions in Lung Alveolar Epithelial Cells |
title_fullStr |
β-Peltoboykinolic Acid from <i>Astilbe rubra</i> Attenuates TGF-β1-Induced Epithelial-to-Mesenchymal Transitions in Lung Alveolar Epithelial Cells |
title_full_unstemmed |
β-Peltoboykinolic Acid from <i>Astilbe rubra</i> Attenuates TGF-β1-Induced Epithelial-to-Mesenchymal Transitions in Lung Alveolar Epithelial Cells |
title_sort |
β-peltoboykinolic acid from <i>astilbe rubra</i> attenuates tgf-β1-induced epithelial-to-mesenchymal transitions in lung alveolar epithelial cells |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2019-07-01 |
description |
Epithelial-to-mesenchymal transition (EMT) is increasingly recognized as contributing to the pathogenesis of idiopathic pulmonary fibrosis. Therefore, novel plant-based natural, active compounds have been sought for the treatment of fibrotic EMT. The aim of the present study was to investigate the inhibitory effects of <i>Astilbe rubra</i> on TGF-β1-induced EMT in lung alveolar epithelial cells (A549). <i>A. rubra</i> was subjected to extraction using 70% ethanol (ARE), and ethanol extracts of the aerial part and that of the rhizome were further partitioned using various solvents. Protein expression and cell motility were investigated to evaluate the inhibitory effects of ARE on EMT. EMT occurred in A549 cells treated with TGF-β1, but was prevented by co-treatment with ARE. The dichloromethane fractions showed the strongest inhibitory effect on TGF-β1-induced EMT. β-Peltoboykinolic acid was isolated from the dichloromethane fractions of <i>A. rubra</i> by activity-oriented isolation. β-Peltoboykinolic acid not only attenuated TGF-β1-induced EMT, but also the overproduction of extracellular matrix components including type I collagen and fibronectin. The Smad pathway activated by TGF-β1 was inhibited by co-treatment with β-peltoboykinolic acid. Taken together, these results indicate that β-peltoboykinolic acid from <i>A. rubra</i> and dichloromethane fractions shows potential as an antifibrotic agent in A549 cells treated with TGF-β1. |
topic |
<i>Astilbe rubra</i> β-peltoboykinolic acid epithelial-mesenchymal transition TGF-β1 lung fibrosis |
url |
https://www.mdpi.com/1420-3049/24/14/2573 |
work_keys_str_mv |
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