Real-time optoacoustic temperature determination on cell cultures during heat exposure: a feasibility study
Objective/Purpose: In order to study the effects of hyperthermia and other temperature-related effects on cells and tissues, determining the precise time/temperature course is crucial. Here we present a non-contact optoacoustic technique, which provides temperatures during heating of cultured cells...
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2019-01-01
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Online Access: | http://dx.doi.org/10.1080/02656736.2019.1590653 |
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doaj-a5009c68faf74e8ca3154ee8ebb06ece2020-11-25T02:34:05ZengTaylor & Francis GroupInternational Journal of Hyperthermia0265-67361464-51572019-01-0136146547110.1080/02656736.2019.15906531590653Real-time optoacoustic temperature determination on cell cultures during heat exposure: a feasibility studyYoko Miura0Eric Seifert1Josua Rehra2Katharina Kern3Dirk Theisen-Kunde4Michael Denton5Ralf Brinkmann6Medical Laser Center LübeckMedical Laser Center LübeckMedical Laser Center LübeckMedical Laser Center LübeckMedical Laser Center LübeckJBSAMedical Laser Center LübeckObjective/Purpose: In order to study the effects of hyperthermia and other temperature-related effects on cells and tissues, determining the precise time/temperature course is crucial. Here we present a non-contact optoacoustic technique, which provides temperatures during heating of cultured cells with scalable temporal and spatial resolution. Methods: A thulium laser (1.94 µm) with a maximum power of 15 W quickly and efficiently heats cells in a culture dish because of low penetration depth (1/e penetration depths of 78 µm) of the radiation in water. A repetitively Q-switched holmium laser (2.1 µm) is used simultaneously to probe temperatures at different locations in the dish by using the photoacoustic effect. Due to thermoelastic expansion of water, pressure waves are emitted and measured with an ultrasonic hydrophone at the side of the dish. The amplitudes of the waves are temperature dependent and can be used to calculate the temperature/time course at any location of probing. Results: We measured temperatures of up to 55 °C with a heating power of 6 W after 10 s, and subsequent lateral temperature profiles over time. Within this profile, temperature fluctuations were found, likely owing to thermal convection and water circulation. By using cultured retinal pigment epithelial cells, it is shown that the probe laser pulses alone cause no biological damage, while immediate cell damage occurs when heating for 10 s at temperatures exceeding 45 °C. Conclusions: This method shows great potential not only as a noninvasive, non-contact method to determine temperature/time responses of cells in culture, but also for complex tissue and other materials.http://dx.doi.org/10.1080/02656736.2019.1590653noninvasive thermometryoptoacousticphotoacousticcell thermal response2 µm laser |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yoko Miura Eric Seifert Josua Rehra Katharina Kern Dirk Theisen-Kunde Michael Denton Ralf Brinkmann |
spellingShingle |
Yoko Miura Eric Seifert Josua Rehra Katharina Kern Dirk Theisen-Kunde Michael Denton Ralf Brinkmann Real-time optoacoustic temperature determination on cell cultures during heat exposure: a feasibility study International Journal of Hyperthermia noninvasive thermometry optoacoustic photoacoustic cell thermal response 2 µm laser |
author_facet |
Yoko Miura Eric Seifert Josua Rehra Katharina Kern Dirk Theisen-Kunde Michael Denton Ralf Brinkmann |
author_sort |
Yoko Miura |
title |
Real-time optoacoustic temperature determination on cell cultures during heat exposure: a feasibility study |
title_short |
Real-time optoacoustic temperature determination on cell cultures during heat exposure: a feasibility study |
title_full |
Real-time optoacoustic temperature determination on cell cultures during heat exposure: a feasibility study |
title_fullStr |
Real-time optoacoustic temperature determination on cell cultures during heat exposure: a feasibility study |
title_full_unstemmed |
Real-time optoacoustic temperature determination on cell cultures during heat exposure: a feasibility study |
title_sort |
real-time optoacoustic temperature determination on cell cultures during heat exposure: a feasibility study |
publisher |
Taylor & Francis Group |
series |
International Journal of Hyperthermia |
issn |
0265-6736 1464-5157 |
publishDate |
2019-01-01 |
description |
Objective/Purpose: In order to study the effects of hyperthermia and other temperature-related effects on cells and tissues, determining the precise time/temperature course is crucial. Here we present a non-contact optoacoustic technique, which provides temperatures during heating of cultured cells with scalable temporal and spatial resolution. Methods: A thulium laser (1.94 µm) with a maximum power of 15 W quickly and efficiently heats cells in a culture dish because of low penetration depth (1/e penetration depths of 78 µm) of the radiation in water. A repetitively Q-switched holmium laser (2.1 µm) is used simultaneously to probe temperatures at different locations in the dish by using the photoacoustic effect. Due to thermoelastic expansion of water, pressure waves are emitted and measured with an ultrasonic hydrophone at the side of the dish. The amplitudes of the waves are temperature dependent and can be used to calculate the temperature/time course at any location of probing. Results: We measured temperatures of up to 55 °C with a heating power of 6 W after 10 s, and subsequent lateral temperature profiles over time. Within this profile, temperature fluctuations were found, likely owing to thermal convection and water circulation. By using cultured retinal pigment epithelial cells, it is shown that the probe laser pulses alone cause no biological damage, while immediate cell damage occurs when heating for 10 s at temperatures exceeding 45 °C. Conclusions: This method shows great potential not only as a noninvasive, non-contact method to determine temperature/time responses of cells in culture, but also for complex tissue and other materials. |
topic |
noninvasive thermometry optoacoustic photoacoustic cell thermal response 2 µm laser |
url |
http://dx.doi.org/10.1080/02656736.2019.1590653 |
work_keys_str_mv |
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