Levels of lycopene β-cyclase 1 modulate carotenoid gene expression and accumulation in Daucus carota.

Plant carotenoids are synthesized and accumulated in plastids through a highly regulated pathway. Lycopene β-cyclase (LCYB) is a key enzyme involved directly in the synthesis of α-carotene and β-carotene through the cyclization of lycopene. Carotenoids are produced in both carrot (Daucus carota) lea...

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Main Authors: Juan Camilo Moreno, Lorena Pizarro, Paulina Fuentes, Michael Handford, Victor Cifuentes, Claudia Stange
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23555569/pdf/?tool=EBI
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spelling doaj-a51a0495e9b64f2cbbc622b56dc1f7232021-03-03T20:24:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0183e5814410.1371/journal.pone.0058144Levels of lycopene β-cyclase 1 modulate carotenoid gene expression and accumulation in Daucus carota.Juan Camilo MorenoLorena PizarroPaulina FuentesMichael HandfordVictor CifuentesClaudia StangePlant carotenoids are synthesized and accumulated in plastids through a highly regulated pathway. Lycopene β-cyclase (LCYB) is a key enzyme involved directly in the synthesis of α-carotene and β-carotene through the cyclization of lycopene. Carotenoids are produced in both carrot (Daucus carota) leaves and reserve roots, and high amounts of α-carotene and β-carotene accumulate in the latter. In some plant models, the presence of different isoforms of carotenogenic genes is associated with an organ-specific function. D. carota harbors two Lcyb genes, of which DcLcyb1 is expressed in leaves and storage roots during carrot development, correlating with an increase in carotenoid levels. In this work, we show that DcLCYB1 is localized in the plastid and that it is a functional enzyme, as demonstrated by heterologous complementation in Escherichia coli and over expression and post transcriptional gene silencing in carrot. Transgenic plants with higher or reduced levels of DcLcyb1 had incremented or reduced levels of chlorophyll, total carotenoids and β-carotene in leaves and in the storage roots, respectively. In addition, changes in the expression of DcLcyb1 are accompanied by a modulation in the expression of key endogenous carotenogenic genes. Our results indicate that DcLcyb1 does not possess an organ specific function and modulate carotenoid gene expression and accumulation in carrot leaves and storage roots.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23555569/pdf/?tool=EBI
collection DOAJ
language English
format Article
sources DOAJ
author Juan Camilo Moreno
Lorena Pizarro
Paulina Fuentes
Michael Handford
Victor Cifuentes
Claudia Stange
spellingShingle Juan Camilo Moreno
Lorena Pizarro
Paulina Fuentes
Michael Handford
Victor Cifuentes
Claudia Stange
Levels of lycopene β-cyclase 1 modulate carotenoid gene expression and accumulation in Daucus carota.
PLoS ONE
author_facet Juan Camilo Moreno
Lorena Pizarro
Paulina Fuentes
Michael Handford
Victor Cifuentes
Claudia Stange
author_sort Juan Camilo Moreno
title Levels of lycopene β-cyclase 1 modulate carotenoid gene expression and accumulation in Daucus carota.
title_short Levels of lycopene β-cyclase 1 modulate carotenoid gene expression and accumulation in Daucus carota.
title_full Levels of lycopene β-cyclase 1 modulate carotenoid gene expression and accumulation in Daucus carota.
title_fullStr Levels of lycopene β-cyclase 1 modulate carotenoid gene expression and accumulation in Daucus carota.
title_full_unstemmed Levels of lycopene β-cyclase 1 modulate carotenoid gene expression and accumulation in Daucus carota.
title_sort levels of lycopene β-cyclase 1 modulate carotenoid gene expression and accumulation in daucus carota.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2013-01-01
description Plant carotenoids are synthesized and accumulated in plastids through a highly regulated pathway. Lycopene β-cyclase (LCYB) is a key enzyme involved directly in the synthesis of α-carotene and β-carotene through the cyclization of lycopene. Carotenoids are produced in both carrot (Daucus carota) leaves and reserve roots, and high amounts of α-carotene and β-carotene accumulate in the latter. In some plant models, the presence of different isoforms of carotenogenic genes is associated with an organ-specific function. D. carota harbors two Lcyb genes, of which DcLcyb1 is expressed in leaves and storage roots during carrot development, correlating with an increase in carotenoid levels. In this work, we show that DcLCYB1 is localized in the plastid and that it is a functional enzyme, as demonstrated by heterologous complementation in Escherichia coli and over expression and post transcriptional gene silencing in carrot. Transgenic plants with higher or reduced levels of DcLcyb1 had incremented or reduced levels of chlorophyll, total carotenoids and β-carotene in leaves and in the storage roots, respectively. In addition, changes in the expression of DcLcyb1 are accompanied by a modulation in the expression of key endogenous carotenogenic genes. Our results indicate that DcLcyb1 does not possess an organ specific function and modulate carotenoid gene expression and accumulation in carrot leaves and storage roots.
url https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23555569/pdf/?tool=EBI
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