Bovine polledness--an autosomal dominant trait with allelic heterogeneity.
The persistent horns are an important trait of speciation for the family Bovidae with complex morphogenesis taking place briefly after birth. The polledness is highly favourable in modern cattle breeding systems but serious animal welfare issues urge for a solution in the production of hornless catt...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2012-01-01
|
Series: | PLoS ONE |
Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22737241/pdf/?tool=EBI |
id |
doaj-a527f6b96d574498b2f0d3851f7732cf |
---|---|
record_format |
Article |
spelling |
doaj-a527f6b96d574498b2f0d3851f7732cf2021-03-04T00:37:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0176e3947710.1371/journal.pone.0039477Bovine polledness--an autosomal dominant trait with allelic heterogeneity.Ivica MedugoracDoris SeichterAlexander GrafIngolf RussHelmut BlumKarl Heinrich GöpelSophie RothammerMartin FörsterStefan KrebsThe persistent horns are an important trait of speciation for the family Bovidae with complex morphogenesis taking place briefly after birth. The polledness is highly favourable in modern cattle breeding systems but serious animal welfare issues urge for a solution in the production of hornless cattle other than dehorning. Although the dominant inhibition of horn morphogenesis was discovered more than 70 years ago, and the causative mutation was mapped almost 20 years ago, its molecular nature remained unknown. Here, we report allelic heterogeneity of the POLLED locus. First, we mapped the POLLED locus to a ∼381-kb interval in a multi-breed case-control design. Targeted re-sequencing of an enlarged candidate interval (547 kb) in 16 sires with known POLLED genotype did not detect a common allele associated with polled status. In eight sires of Alpine and Scottish origin (four polled versus four horned), we identified a single candidate mutation, a complex 202 bp insertion-deletion event that showed perfect association to the polled phenotype in various European cattle breeds, except Holstein-Friesian. The analysis of the same candidate interval in eight Holsteins identified five candidate variants which segregate as a 260 kb haplotype also perfectly associated with the POLLED gene without recombination or interference with the 202 bp insertion-deletion. We further identified bulls which are progeny tested as homozygous polled but bearing both, 202 bp insertion-deletion and Friesian haplotype. The distribution of genotypes of the two putative POLLED alleles in large semi-random sample (1,261 animals) supports the hypothesis of two independent mutations.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22737241/pdf/?tool=EBI |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ivica Medugorac Doris Seichter Alexander Graf Ingolf Russ Helmut Blum Karl Heinrich Göpel Sophie Rothammer Martin Förster Stefan Krebs |
spellingShingle |
Ivica Medugorac Doris Seichter Alexander Graf Ingolf Russ Helmut Blum Karl Heinrich Göpel Sophie Rothammer Martin Förster Stefan Krebs Bovine polledness--an autosomal dominant trait with allelic heterogeneity. PLoS ONE |
author_facet |
Ivica Medugorac Doris Seichter Alexander Graf Ingolf Russ Helmut Blum Karl Heinrich Göpel Sophie Rothammer Martin Förster Stefan Krebs |
author_sort |
Ivica Medugorac |
title |
Bovine polledness--an autosomal dominant trait with allelic heterogeneity. |
title_short |
Bovine polledness--an autosomal dominant trait with allelic heterogeneity. |
title_full |
Bovine polledness--an autosomal dominant trait with allelic heterogeneity. |
title_fullStr |
Bovine polledness--an autosomal dominant trait with allelic heterogeneity. |
title_full_unstemmed |
Bovine polledness--an autosomal dominant trait with allelic heterogeneity. |
title_sort |
bovine polledness--an autosomal dominant trait with allelic heterogeneity. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
The persistent horns are an important trait of speciation for the family Bovidae with complex morphogenesis taking place briefly after birth. The polledness is highly favourable in modern cattle breeding systems but serious animal welfare issues urge for a solution in the production of hornless cattle other than dehorning. Although the dominant inhibition of horn morphogenesis was discovered more than 70 years ago, and the causative mutation was mapped almost 20 years ago, its molecular nature remained unknown. Here, we report allelic heterogeneity of the POLLED locus. First, we mapped the POLLED locus to a ∼381-kb interval in a multi-breed case-control design. Targeted re-sequencing of an enlarged candidate interval (547 kb) in 16 sires with known POLLED genotype did not detect a common allele associated with polled status. In eight sires of Alpine and Scottish origin (four polled versus four horned), we identified a single candidate mutation, a complex 202 bp insertion-deletion event that showed perfect association to the polled phenotype in various European cattle breeds, except Holstein-Friesian. The analysis of the same candidate interval in eight Holsteins identified five candidate variants which segregate as a 260 kb haplotype also perfectly associated with the POLLED gene without recombination or interference with the 202 bp insertion-deletion. We further identified bulls which are progeny tested as homozygous polled but bearing both, 202 bp insertion-deletion and Friesian haplotype. The distribution of genotypes of the two putative POLLED alleles in large semi-random sample (1,261 animals) supports the hypothesis of two independent mutations. |
url |
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22737241/pdf/?tool=EBI |
work_keys_str_mv |
AT ivicamedugorac bovinepollednessanautosomaldominanttraitwithallelicheterogeneity AT dorisseichter bovinepollednessanautosomaldominanttraitwithallelicheterogeneity AT alexandergraf bovinepollednessanautosomaldominanttraitwithallelicheterogeneity AT ingolfruss bovinepollednessanautosomaldominanttraitwithallelicheterogeneity AT helmutblum bovinepollednessanautosomaldominanttraitwithallelicheterogeneity AT karlheinrichgopel bovinepollednessanautosomaldominanttraitwithallelicheterogeneity AT sophierothammer bovinepollednessanautosomaldominanttraitwithallelicheterogeneity AT martinforster bovinepollednessanautosomaldominanttraitwithallelicheterogeneity AT stefankrebs bovinepollednessanautosomaldominanttraitwithallelicheterogeneity |
_version_ |
1714810118234701824 |