Potential environmental risks of nanopesticides: Application of Cu(OH)2 nanopesticides to soil mitigates the degradation of neonicotinoid thiacloprid

• Main Authors: Xiaoxia Zhang, Zhenlan Xu, Mansha Wu, Xiaoting Qian, Daohui Lin, Hangjun Zhang, Juan Tang, Tao Zeng, Weijun Yao, Juliane Filser, Lingxiangyu Li, Virender K. Sharma
• Format: Article
• Language: English
• Published: Elsevier 2019-08-01
• Series: Environment International
• Online Access: http://www.sciencedirect.com/science/article/pii/S0160412019309705

Cu(OH)2 nanopesticides and organic insecticides are continuously applied to soil at a temporal interval, while knowledge about the impact of Cu(OH)2 nanopesticides on organic insecticides degradation is currently scarce, resulting in poorly comprehensive evaluation of the potential environmental risks of Cu(OH)2 nanopesticides. Herein, a commercial Cu(OH)2 nanopesticide formulation (NPF), the active ingredient of NPF (AI-NPF), the prepared Cu(OH)2 nanotubes (NT) with comparable morphology and size to AI-NPF, and CuSO4 were respectively applied to soil at normal doses (0.5, 5 and 50 mg/kg), followed by an application of neonicotinoid thiacloprid after an interval of 21 d, showing that NPF at doses of 5 and 50 mg/kg significantly (p < 0.05) mitigated thiacloprid degradation compared to control and CuSO4. Furthermore, AI-NPF was the primary component that contributed to the mitigation effect of NPF, which was also validated by the NT. Large differences in the degradation efficiency of thiacloprid in sterilized and unsterilized soils with Cu(OH)2 nanopesticides suggested that biodegradation was the primary process responsible for thiacloprid degradation, especially as chemical degradation was negligible. Besides a decrease of thiacloprid bioavailability due to adsorption by Cu(OH)2 nanopesticides, we demonstrated that Cu(OH)2 nanopesticides changed soil microbial communities, reduced nitrile hydratase activity and down-regulated thiacloprid-degradative nth gene abundance, which thus mitigated thiacloprid biodegradation. Clearly, this study shed light on the potential environmental risks of Cu(OH)2 nanopesticide. Keywords: Cu(OH)2 nanopesticide, Thiacloprid degradation, Adsorption, Microbial communities, Nitrile hydratase activity, Gene abundance