Skeletal Muscle Regeneration on Protein-Grafted and Microchannel-Patterned Scaffold for Hypopharyngeal Tissue Engineering

Skeletal Muscle Regeneration on Protein-Grafted and Microchannel-Patterned Scaffold for Hypopharyngeal Tissue Engineering

In the field of tissue engineering, polymeric materials with high biocompatibility like polylactic acid and polyglycolic acid have been widely used for fabricating living constructs. For hypopharynx tissue engineering, skeletal muscle is one important functional part of the whole organ, which assemb...

Full description

Bibliographic Details
Main Authors: Zhisen Shen, Shanshan Guo, Dong Ye, Jingjing Chen, Cheng Kang, Shejie Qiu, Dakai Lu, Qun Li, Kunjie Xu, Jingjing Lv, Yabin Zhu
Format: Article
Language:English
Published: Hindawi Limited 2013-01-01
Series:BioMed Research International
Online Access:http://dx.doi.org/10.1155/2013/146953
id doaj-a53d962b840b48abb90fad73e32068d2
record_format Article
spelling doaj-a53d962b840b48abb90fad73e32068d22020-11-24T22:43:26ZengHindawi LimitedBioMed Research International2314-61332314-61412013-01-01201310.1155/2013/146953146953Skeletal Muscle Regeneration on Protein-Grafted and Microchannel-Patterned Scaffold for Hypopharyngeal Tissue EngineeringZhisen Shen0Shanshan Guo1Dong Ye2Jingjing Chen3Cheng Kang4Shejie Qiu5Dakai Lu6Qun Li7Kunjie Xu8Jingjing Lv9Yabin Zhu10Department of Otorhinolaryngology of Lihuili Hospital, Ningbo University, Ningbo 315211, ChinaThe Medical School, Ningbo University, Ningbo 315211, ChinaDepartment of Otorhinolaryngology of Lihuili Hospital, Ningbo University, Ningbo 315211, ChinaThe Medical School, Ningbo University, Ningbo 315211, ChinaThe Medical School, Ningbo University, Ningbo 315211, ChinaDepartment of Otorhinolaryngology of Lihuili Hospital, Ningbo University, Ningbo 315211, ChinaThe Medical School, Ningbo University, Ningbo 315211, ChinaThe Medical School, Ningbo University, Ningbo 315211, ChinaThe Medical School, Ningbo University, Ningbo 315211, ChinaThe Medical School, Ningbo University, Ningbo 315211, ChinaThe Medical School, Ningbo University, Ningbo 315211, ChinaIn the field of tissue engineering, polymeric materials with high biocompatibility like polylactic acid and polyglycolic acid have been widely used for fabricating living constructs. For hypopharynx tissue engineering, skeletal muscle is one important functional part of the whole organ, which assembles the unidirectionally aligned myotubes. In this study, a polyurethane (PU) scaffold with microchannel patterns was used to provide aligning guidance for the seeded human myoblasts. Due to the low hydrophilicity of PU, the scaffold was grafted with silk fibroin (PU-SF) or gelatin (PU-Gel) to improve its cell adhesion properties. Scaffolds were observed to degrade slowly over time, and their mechanical properties and hydrophilicities were improved through the surface grafting. Also, the myoblasts seeded on PU-SF had the higher proliferative rate and better differentiation compared with those on the control or PU-Gel. Our results demonstrate that polyurethane scaffolds seeded with myoblasts hold promise to guide hypopharynx muscle regeneration.http://dx.doi.org/10.1155/2013/146953
collection DOAJ
language English
format Article
sources DOAJ
author Zhisen Shen
Shanshan Guo
Dong Ye
Jingjing Chen
Cheng Kang
Shejie Qiu
Dakai Lu
Qun Li
Kunjie Xu
Jingjing Lv
Yabin Zhu
spellingShingle Zhisen Shen
Shanshan Guo
Dong Ye
Jingjing Chen
Cheng Kang
Shejie Qiu
Dakai Lu
Qun Li
Kunjie Xu
Jingjing Lv
Yabin Zhu
Skeletal Muscle Regeneration on Protein-Grafted and Microchannel-Patterned Scaffold for Hypopharyngeal Tissue Engineering
BioMed Research International
author_facet Zhisen Shen
Shanshan Guo
Dong Ye
Jingjing Chen
Cheng Kang
Shejie Qiu
Dakai Lu
Qun Li
Kunjie Xu
Jingjing Lv
Yabin Zhu
author_sort Zhisen Shen
title Skeletal Muscle Regeneration on Protein-Grafted and Microchannel-Patterned Scaffold for Hypopharyngeal Tissue Engineering
title_short Skeletal Muscle Regeneration on Protein-Grafted and Microchannel-Patterned Scaffold for Hypopharyngeal Tissue Engineering
title_full Skeletal Muscle Regeneration on Protein-Grafted and Microchannel-Patterned Scaffold for Hypopharyngeal Tissue Engineering
title_fullStr Skeletal Muscle Regeneration on Protein-Grafted and Microchannel-Patterned Scaffold for Hypopharyngeal Tissue Engineering
title_full_unstemmed Skeletal Muscle Regeneration on Protein-Grafted and Microchannel-Patterned Scaffold for Hypopharyngeal Tissue Engineering
title_sort skeletal muscle regeneration on protein-grafted and microchannel-patterned scaffold for hypopharyngeal tissue engineering
publisher Hindawi Limited
series BioMed Research International
issn 2314-6133
2314-6141
publishDate 2013-01-01
description In the field of tissue engineering, polymeric materials with high biocompatibility like polylactic acid and polyglycolic acid have been widely used for fabricating living constructs. For hypopharynx tissue engineering, skeletal muscle is one important functional part of the whole organ, which assembles the unidirectionally aligned myotubes. In this study, a polyurethane (PU) scaffold with microchannel patterns was used to provide aligning guidance for the seeded human myoblasts. Due to the low hydrophilicity of PU, the scaffold was grafted with silk fibroin (PU-SF) or gelatin (PU-Gel) to improve its cell adhesion properties. Scaffolds were observed to degrade slowly over time, and their mechanical properties and hydrophilicities were improved through the surface grafting. Also, the myoblasts seeded on PU-SF had the higher proliferative rate and better differentiation compared with those on the control or PU-Gel. Our results demonstrate that polyurethane scaffolds seeded with myoblasts hold promise to guide hypopharynx muscle regeneration.
url http://dx.doi.org/10.1155/2013/146953
work_keys_str_mv AT zhisenshen skeletalmuscleregenerationonproteingraftedandmicrochannelpatternedscaffoldforhypopharyngealtissueengineering
AT shanshanguo skeletalmuscleregenerationonproteingraftedandmicrochannelpatternedscaffoldforhypopharyngealtissueengineering
AT dongye skeletalmuscleregenerationonproteingraftedandmicrochannelpatternedscaffoldforhypopharyngealtissueengineering
AT jingjingchen skeletalmuscleregenerationonproteingraftedandmicrochannelpatternedscaffoldforhypopharyngealtissueengineering
AT chengkang skeletalmuscleregenerationonproteingraftedandmicrochannelpatternedscaffoldforhypopharyngealtissueengineering
AT shejieqiu skeletalmuscleregenerationonproteingraftedandmicrochannelpatternedscaffoldforhypopharyngealtissueengineering
AT dakailu skeletalmuscleregenerationonproteingraftedandmicrochannelpatternedscaffoldforhypopharyngealtissueengineering
AT qunli skeletalmuscleregenerationonproteingraftedandmicrochannelpatternedscaffoldforhypopharyngealtissueengineering
AT kunjiexu skeletalmuscleregenerationonproteingraftedandmicrochannelpatternedscaffoldforhypopharyngealtissueengineering
AT jingjinglv skeletalmuscleregenerationonproteingraftedandmicrochannelpatternedscaffoldforhypopharyngealtissueengineering
AT yabinzhu skeletalmuscleregenerationonproteingraftedandmicrochannelpatternedscaffoldforhypopharyngealtissueengineering
_version_ 1725695969816739840

Similar Items