Interphase Microtubules Safeguard Mitotic Progression by Suppressing an Aurora B-Dependent Arrest Induced by DNA Replication Stress
Summary: The segregation of chromosomes is a critical step during cell division. This process is driven by the elongation of spindle microtubules and is tightly regulated by checkpoint mechanisms. It is unknown whether microtubules affect checkpoint responses as passive contributors or active regula...
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doaj-a541861d127246ceaa438f4b0a8be5d52020-11-25T00:46:47ZengElsevierCell Reports2211-12472019-03-01261128752889.e3Interphase Microtubules Safeguard Mitotic Progression by Suppressing an Aurora B-Dependent Arrest Induced by DNA Replication StressGuillaume Laflamme0Shannon Sim1Allen Leary2Mirela Pascariu3Jackie Vogel4Damien D’Amours5Institute for Research in Immunology and Cancer, Université de Montréal, C.P. 6128, Succursale Centre-ville, Montréal, QC H3C 3J7, CanadaDepartment of Biology, McGill University, 3649 Promenade Sir William Osler, Montreal, QC H3G 0B1, CanadaDepartment of Biology, McGill University, 3649 Promenade Sir William Osler, Montreal, QC H3G 0B1, CanadaInstitute for Research in Immunology and Cancer, Université de Montréal, C.P. 6128, Succursale Centre-ville, Montréal, QC H3C 3J7, CanadaDepartment of Biology, McGill University, 3649 Promenade Sir William Osler, Montreal, QC H3G 0B1, CanadaInstitute for Research in Immunology and Cancer, Université de Montréal, C.P. 6128, Succursale Centre-ville, Montréal, QC H3C 3J7, Canada; Ottawa Institute of Systems Biology, Department of Cellular and Molecular Medicine, University of Ottawa, Roger Guindon Hall, 451 Smyth Rd, Ottawa, ON K1H 8M5, Canada; Corresponding authorSummary: The segregation of chromosomes is a critical step during cell division. This process is driven by the elongation of spindle microtubules and is tightly regulated by checkpoint mechanisms. It is unknown whether microtubules affect checkpoint responses as passive contributors or active regulators of the process. We show here that interphase microtubules are essential to temporally restrict the effects of DNA replication stress to S phase in Saccharomyces cerevisiae. Tubulin mutants hypersensitive to DNA damage experience a strong but delayed mitotic checkpoint arrest after exposure to genotoxic stress in S phase. This untimely arrest is dependent on the Aurora B kinase but, surprisingly, not on the DNA damage checkpoint. Impaired microtubule-kinetochore interaction is the apparent cause for this unusual phenotype. Collectively, our results reveal that core components of microtubules potentiate the detection of DNA lesions created in S phase, thereby suppressing untimely activation of mitotic checkpoints after DNA replication stress. : DNA replication stress is detected in S phase by a dedicated checkpoint machinery that ensures cells do not enter mitosis with damaged chromosomes. Laflamme et al. found that microtubules limit the effects of replication stress to S phase by modulating the levels of tension at sister kinetochores of replicated chromatids. Keywords: microtubules, Aurora B kinase, replication stress, DNA damage, kinetochore, mitosis, checkpointhttp://www.sciencedirect.com/science/article/pii/S221112471930230X |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Guillaume Laflamme Shannon Sim Allen Leary Mirela Pascariu Jackie Vogel Damien D’Amours |
spellingShingle |
Guillaume Laflamme Shannon Sim Allen Leary Mirela Pascariu Jackie Vogel Damien D’Amours Interphase Microtubules Safeguard Mitotic Progression by Suppressing an Aurora B-Dependent Arrest Induced by DNA Replication Stress Cell Reports |
author_facet |
Guillaume Laflamme Shannon Sim Allen Leary Mirela Pascariu Jackie Vogel Damien D’Amours |
author_sort |
Guillaume Laflamme |
title |
Interphase Microtubules Safeguard Mitotic Progression by Suppressing an Aurora B-Dependent Arrest Induced by DNA Replication Stress |
title_short |
Interphase Microtubules Safeguard Mitotic Progression by Suppressing an Aurora B-Dependent Arrest Induced by DNA Replication Stress |
title_full |
Interphase Microtubules Safeguard Mitotic Progression by Suppressing an Aurora B-Dependent Arrest Induced by DNA Replication Stress |
title_fullStr |
Interphase Microtubules Safeguard Mitotic Progression by Suppressing an Aurora B-Dependent Arrest Induced by DNA Replication Stress |
title_full_unstemmed |
Interphase Microtubules Safeguard Mitotic Progression by Suppressing an Aurora B-Dependent Arrest Induced by DNA Replication Stress |
title_sort |
interphase microtubules safeguard mitotic progression by suppressing an aurora b-dependent arrest induced by dna replication stress |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2019-03-01 |
description |
Summary: The segregation of chromosomes is a critical step during cell division. This process is driven by the elongation of spindle microtubules and is tightly regulated by checkpoint mechanisms. It is unknown whether microtubules affect checkpoint responses as passive contributors or active regulators of the process. We show here that interphase microtubules are essential to temporally restrict the effects of DNA replication stress to S phase in Saccharomyces cerevisiae. Tubulin mutants hypersensitive to DNA damage experience a strong but delayed mitotic checkpoint arrest after exposure to genotoxic stress in S phase. This untimely arrest is dependent on the Aurora B kinase but, surprisingly, not on the DNA damage checkpoint. Impaired microtubule-kinetochore interaction is the apparent cause for this unusual phenotype. Collectively, our results reveal that core components of microtubules potentiate the detection of DNA lesions created in S phase, thereby suppressing untimely activation of mitotic checkpoints after DNA replication stress. : DNA replication stress is detected in S phase by a dedicated checkpoint machinery that ensures cells do not enter mitosis with damaged chromosomes. Laflamme et al. found that microtubules limit the effects of replication stress to S phase by modulating the levels of tension at sister kinetochores of replicated chromatids. Keywords: microtubules, Aurora B kinase, replication stress, DNA damage, kinetochore, mitosis, checkpoint |
url |
http://www.sciencedirect.com/science/article/pii/S221112471930230X |
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