Quantification of seed–soil contact of sugar beet (Beta vulgaris) using X-ray Computed Tomography

• Main Authors: Sebastian Blunk, Ali Hafeez Malik, Martine I. de Heer, Tobias Ekblad, Jennifer Bussell, Debbie Sparkes, Kenneth Fredlund, Craig J. Sturrock, Sacha J. Mooney
• Format: Article
• Language: English
• Published: BMC 2017-08-01
• Series: Plant Methods
• Subjects: Sugar beet; Germination; Seeds; Seed–soil contact; X-ray Computed Tomography
• Online Access: http://link.springer.com/article/10.1186/s13007-017-0220-4

Abstract Background Seed–soil contact is important to ensure successful germination, however, there is a paucity of reported studies that have quantified the microstructure at and around this critical interface, mainly due to the opacity of soil. Results Here we describe a novel methodology to non-destructively calculate the seed–soil contact area using X-ray Computed Tomography. Under controlled conditions, we observed that seed–soil contact was strongly influenced by the size and type of seed, with a seed–soil contact of ca. 15% for naked sugar beet seeds compared to ca. 32% for pelleted and coated seeds. Similar results were obtained for seeds sampled from the field albeit with a higher spatial variability. Conclusions By application of this new quantification method it is hoped seed enhancement technologies can be optimised and ultimately seedbed preparation improved to ensure better germination.