miR-29a/b enhances cell migration and invasion in nasopharyngeal carcinoma progression by regulating SPARC and COL3A1 gene expression.
Nasopharyngeal carcinoma (NPC) is a malignant tumor associated with a genetic predisposition, Epstein-Barr virus infection and chromosomal abnormalities. Recently, several miRNAs have been shown to target specific mRNAs to regulate NPC development and progression. However, the involvement of miRNAs...
Main Authors: | , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2015-01-01
|
Series: | PLoS ONE |
Online Access: | https://doi.org/10.1371/journal.pone.0120969 |
id |
doaj-a559ed4049b34de98013fc2f75c33c9f |
---|---|
record_format |
Article |
spelling |
doaj-a559ed4049b34de98013fc2f75c33c9f2021-03-03T20:08:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01103e012096910.1371/journal.pone.0120969miR-29a/b enhances cell migration and invasion in nasopharyngeal carcinoma progression by regulating SPARC and COL3A1 gene expression.Feifei QiuRui SunNing DengTianyu GuoYange CaoYing YuXuejun WangBingcheng ZouSongmei ZhangTao JingTao LingJun XieQing ZhangNasopharyngeal carcinoma (NPC) is a malignant tumor associated with a genetic predisposition, Epstein-Barr virus infection and chromosomal abnormalities. Recently, several miRNAs have been shown to target specific mRNAs to regulate NPC development and progression. However, the involvement of miRNAs in processes leading to NPC migration and invasion remains to be elucidated. We predicted that miR-29a/b are associated with dysregulated genes controlling NPC through an integrated interaction network of miRNAs and genes. miR-29a/b over-expression in NPC cell lines had no significant effect on proliferation, whereas miR-29b mildly increased the percentage of cells in the G1 phase with a concomitant decrease in the percentage of cells in S phase. Furthermore, we demonstrated that miR-29a/b might be responsible for increasing S18 cell migration and invasion, and only COL3A1 was identified as a direct target of miR-29b despite the fact that both SPARC and COL3A1 were inhibited by miR-29a/b over-expression. Meanwhile, SPARC proteins were increased in metastatic NPC tissue and are involved in NPC progression. Unexpectedly, we identified that miRNA-29b expression was elevated in the serum of NPC patients with a high risk of metastasis. The 5-year actuarial overall survival rates in NPC patients with high serum miR-29b expression was significantly shorter than those with low serum miR-29b expression; therefore, serum miR-29b expression could be a promising prognostic marker.https://doi.org/10.1371/journal.pone.0120969 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Feifei Qiu Rui Sun Ning Deng Tianyu Guo Yange Cao Ying Yu Xuejun Wang Bingcheng Zou Songmei Zhang Tao Jing Tao Ling Jun Xie Qing Zhang |
spellingShingle |
Feifei Qiu Rui Sun Ning Deng Tianyu Guo Yange Cao Ying Yu Xuejun Wang Bingcheng Zou Songmei Zhang Tao Jing Tao Ling Jun Xie Qing Zhang miR-29a/b enhances cell migration and invasion in nasopharyngeal carcinoma progression by regulating SPARC and COL3A1 gene expression. PLoS ONE |
author_facet |
Feifei Qiu Rui Sun Ning Deng Tianyu Guo Yange Cao Ying Yu Xuejun Wang Bingcheng Zou Songmei Zhang Tao Jing Tao Ling Jun Xie Qing Zhang |
author_sort |
Feifei Qiu |
title |
miR-29a/b enhances cell migration and invasion in nasopharyngeal carcinoma progression by regulating SPARC and COL3A1 gene expression. |
title_short |
miR-29a/b enhances cell migration and invasion in nasopharyngeal carcinoma progression by regulating SPARC and COL3A1 gene expression. |
title_full |
miR-29a/b enhances cell migration and invasion in nasopharyngeal carcinoma progression by regulating SPARC and COL3A1 gene expression. |
title_fullStr |
miR-29a/b enhances cell migration and invasion in nasopharyngeal carcinoma progression by regulating SPARC and COL3A1 gene expression. |
title_full_unstemmed |
miR-29a/b enhances cell migration and invasion in nasopharyngeal carcinoma progression by regulating SPARC and COL3A1 gene expression. |
title_sort |
mir-29a/b enhances cell migration and invasion in nasopharyngeal carcinoma progression by regulating sparc and col3a1 gene expression. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2015-01-01 |
description |
Nasopharyngeal carcinoma (NPC) is a malignant tumor associated with a genetic predisposition, Epstein-Barr virus infection and chromosomal abnormalities. Recently, several miRNAs have been shown to target specific mRNAs to regulate NPC development and progression. However, the involvement of miRNAs in processes leading to NPC migration and invasion remains to be elucidated. We predicted that miR-29a/b are associated with dysregulated genes controlling NPC through an integrated interaction network of miRNAs and genes. miR-29a/b over-expression in NPC cell lines had no significant effect on proliferation, whereas miR-29b mildly increased the percentage of cells in the G1 phase with a concomitant decrease in the percentage of cells in S phase. Furthermore, we demonstrated that miR-29a/b might be responsible for increasing S18 cell migration and invasion, and only COL3A1 was identified as a direct target of miR-29b despite the fact that both SPARC and COL3A1 were inhibited by miR-29a/b over-expression. Meanwhile, SPARC proteins were increased in metastatic NPC tissue and are involved in NPC progression. Unexpectedly, we identified that miRNA-29b expression was elevated in the serum of NPC patients with a high risk of metastasis. The 5-year actuarial overall survival rates in NPC patients with high serum miR-29b expression was significantly shorter than those with low serum miR-29b expression; therefore, serum miR-29b expression could be a promising prognostic marker. |
url |
https://doi.org/10.1371/journal.pone.0120969 |
work_keys_str_mv |
AT feifeiqiu mir29abenhancescellmigrationandinvasioninnasopharyngealcarcinomaprogressionbyregulatingsparcandcol3a1geneexpression AT ruisun mir29abenhancescellmigrationandinvasioninnasopharyngealcarcinomaprogressionbyregulatingsparcandcol3a1geneexpression AT ningdeng mir29abenhancescellmigrationandinvasioninnasopharyngealcarcinomaprogressionbyregulatingsparcandcol3a1geneexpression AT tianyuguo mir29abenhancescellmigrationandinvasioninnasopharyngealcarcinomaprogressionbyregulatingsparcandcol3a1geneexpression AT yangecao mir29abenhancescellmigrationandinvasioninnasopharyngealcarcinomaprogressionbyregulatingsparcandcol3a1geneexpression AT yingyu mir29abenhancescellmigrationandinvasioninnasopharyngealcarcinomaprogressionbyregulatingsparcandcol3a1geneexpression AT xuejunwang mir29abenhancescellmigrationandinvasioninnasopharyngealcarcinomaprogressionbyregulatingsparcandcol3a1geneexpression AT bingchengzou mir29abenhancescellmigrationandinvasioninnasopharyngealcarcinomaprogressionbyregulatingsparcandcol3a1geneexpression AT songmeizhang mir29abenhancescellmigrationandinvasioninnasopharyngealcarcinomaprogressionbyregulatingsparcandcol3a1geneexpression AT taojing mir29abenhancescellmigrationandinvasioninnasopharyngealcarcinomaprogressionbyregulatingsparcandcol3a1geneexpression AT taoling mir29abenhancescellmigrationandinvasioninnasopharyngealcarcinomaprogressionbyregulatingsparcandcol3a1geneexpression AT junxie mir29abenhancescellmigrationandinvasioninnasopharyngealcarcinomaprogressionbyregulatingsparcandcol3a1geneexpression AT qingzhang mir29abenhancescellmigrationandinvasioninnasopharyngealcarcinomaprogressionbyregulatingsparcandcol3a1geneexpression |
_version_ |
1714823871905923072 |