Efficient CRISPR/Cas9-Mediated Knockout of an Endogenous <i>PHYTOENE DESATURASE</i> Gene in T1 Progeny of Apomictic <i>Hieracium</i> Enables New Strategies for Apomixis Gene Identification

Efficient CRISPR/Cas9-Mediated Knockout of an Endogenous <i>PHYTOENE DESATURASE</i> Gene in T1 Progeny of Apomictic <i>Hieracium</i> Enables New Strategies for Apomixis Gene Identification

Most <i>Hieracium</i> subgenus <i>Pilosella</i> species are self-incompatible. Some undergo facultative apomixis where most seeds form asexually with a maternal genotype. Most embryo sacs develop by mitosis, without meiosis and seeds form without fertilization. Apomixis is co...

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Bibliographic Details
Main Authors: Sam W. Henderson, Steven T. Henderson, Marc Goetz, Anna M. G. Koltunow
Format: Article
Language:English
Published: MDPI AG 2020-09-01
Series:Genes
Subjects:
<i>Hieracium piloselloides</i>
apomixis
CRISPR/Cas9
<i>PHYTOENE DESATURASE</i> (<i>PDS</i>)
amplicon sequencing
genome editing
Online Access:https://www.mdpi.com/2073-4425/11/9/1064

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