Time course study of the response to LPS targeting the pig immune gene networks
Abstract Background Stress is a generic term used to describe non-specific responses of the body to all kinds of challenges. A very large variability in the response can be observed across individuals, depending on numerous conditioning factors like genetics, early influences and life history. As a...
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doaj-a57ce1d8a94f4814b96c1ee70134e6f62020-11-24T23:28:07ZengBMCBMC Genomics1471-21642017-12-0118111410.1186/s12864-017-4363-5Time course study of the response to LPS targeting the pig immune gene networksElena Terenina0Valérie Sautron1Caroline Ydier2Darya Bazovkina3Amélie Sevin-Pujol4Laure Gress5Yannick Lippi6Claire Naylies7Yvon Billon8Laurence Liaubet9Pierre Mormede10Nathalie Villa-Vialaneix11INRA, UMR 1388 GenPhySE, Université de ToulouseINRA, UMR 1388 GenPhySE, Université de ToulouseINRA, UMR 1388 GenPhySE, Université de ToulouseDepartment of Behavioral Neurogenomics, Siberian Branch of the Russian Academy of SciencesINRA, UMR 1388 GenPhySE, Université de ToulouseINRA, UMR 1388 GenPhySE, Université de ToulouseToxalim (Research Centre in Food Toxicology), Université de ToulouseToxalim (Research Centre in Food Toxicology), Université de ToulouseINRA, UE 1372 GenESIINRA, UMR 1388 GenPhySE, Université de ToulouseINRA, UMR 1388 GenPhySE, Université de ToulouseMIAT, Université de ToulouseAbstract Background Stress is a generic term used to describe non-specific responses of the body to all kinds of challenges. A very large variability in the response can be observed across individuals, depending on numerous conditioning factors like genetics, early influences and life history. As a result, there is a wide range of individual vulnerability and resilience to stress, also called robustness. The importance of robustness-related traits in breeding strategies is increasing progressively towards the production of animals with a high level of production under a wide range of climatic conditions and management systems, together with a lower environmental impact and a high level of animal welfare. The present study aims at describing blood transcriptomic, hormonal, and metabolic responses of pigs to a systemic challenge using lipopolysaccharide (LPS). The objective is to analyze the individual variation of the biological responses in relation to the activity of the HPA axis measured by the levels of plasma cortisol after LPS and ACTH in 120 juvenile Large White (LW) pigs. The kinetics of the response was measured with biological variables and whole blood gene expression at 4 time points. A multilevel statistical analysis was used to take into account the longitudinal aspect of the data. Results Cortisol level reaches its peak 4 h after LPS injection. The characteristic changes of white blood cell count to LPS were observed, with a decrease of total count, maximal at t=+4 h, and the mirror changes in the respective proportions of lymphocytes and granulocytes. The lymphocytes / granulocytes ratio was maximal at t=+1 h. An integrative statistical approach was used and provided a set of candidate genes for kinetic studies and ongoing complementary studies focused on the LPS-stimulated inflammatory response. Conclusions The present study demonstrates the specific biomarkers indicative of an inflammation in swine. Furthermore, these stress responses persist for prolonged periods of time and at significant expression levels, making them good candidate markers for evaluating the efficacy of anti-inflammatory drugs.http://link.springer.com/article/10.1186/s12864-017-4363-5StressHypothalamic-pituitary-adrenal (HPA) axisCortisolTime-courseSystems biologyMicroarray |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Elena Terenina Valérie Sautron Caroline Ydier Darya Bazovkina Amélie Sevin-Pujol Laure Gress Yannick Lippi Claire Naylies Yvon Billon Laurence Liaubet Pierre Mormede Nathalie Villa-Vialaneix |
spellingShingle |
Elena Terenina Valérie Sautron Caroline Ydier Darya Bazovkina Amélie Sevin-Pujol Laure Gress Yannick Lippi Claire Naylies Yvon Billon Laurence Liaubet Pierre Mormede Nathalie Villa-Vialaneix Time course study of the response to LPS targeting the pig immune gene networks BMC Genomics Stress Hypothalamic-pituitary-adrenal (HPA) axis Cortisol Time-course Systems biology Microarray |
author_facet |
Elena Terenina Valérie Sautron Caroline Ydier Darya Bazovkina Amélie Sevin-Pujol Laure Gress Yannick Lippi Claire Naylies Yvon Billon Laurence Liaubet Pierre Mormede Nathalie Villa-Vialaneix |
author_sort |
Elena Terenina |
title |
Time course study of the response to LPS targeting the pig immune gene networks |
title_short |
Time course study of the response to LPS targeting the pig immune gene networks |
title_full |
Time course study of the response to LPS targeting the pig immune gene networks |
title_fullStr |
Time course study of the response to LPS targeting the pig immune gene networks |
title_full_unstemmed |
Time course study of the response to LPS targeting the pig immune gene networks |
title_sort |
time course study of the response to lps targeting the pig immune gene networks |
publisher |
BMC |
series |
BMC Genomics |
issn |
1471-2164 |
publishDate |
2017-12-01 |
description |
Abstract Background Stress is a generic term used to describe non-specific responses of the body to all kinds of challenges. A very large variability in the response can be observed across individuals, depending on numerous conditioning factors like genetics, early influences and life history. As a result, there is a wide range of individual vulnerability and resilience to stress, also called robustness. The importance of robustness-related traits in breeding strategies is increasing progressively towards the production of animals with a high level of production under a wide range of climatic conditions and management systems, together with a lower environmental impact and a high level of animal welfare. The present study aims at describing blood transcriptomic, hormonal, and metabolic responses of pigs to a systemic challenge using lipopolysaccharide (LPS). The objective is to analyze the individual variation of the biological responses in relation to the activity of the HPA axis measured by the levels of plasma cortisol after LPS and ACTH in 120 juvenile Large White (LW) pigs. The kinetics of the response was measured with biological variables and whole blood gene expression at 4 time points. A multilevel statistical analysis was used to take into account the longitudinal aspect of the data. Results Cortisol level reaches its peak 4 h after LPS injection. The characteristic changes of white blood cell count to LPS were observed, with a decrease of total count, maximal at t=+4 h, and the mirror changes in the respective proportions of lymphocytes and granulocytes. The lymphocytes / granulocytes ratio was maximal at t=+1 h. An integrative statistical approach was used and provided a set of candidate genes for kinetic studies and ongoing complementary studies focused on the LPS-stimulated inflammatory response. Conclusions The present study demonstrates the specific biomarkers indicative of an inflammation in swine. Furthermore, these stress responses persist for prolonged periods of time and at significant expression levels, making them good candidate markers for evaluating the efficacy of anti-inflammatory drugs. |
topic |
Stress Hypothalamic-pituitary-adrenal (HPA) axis Cortisol Time-course Systems biology Microarray |
url |
http://link.springer.com/article/10.1186/s12864-017-4363-5 |
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