Post‐eclosion temperature effects on insect cuticular hydrocarbon profiles
Abstract The insect cuticle is the interface between internal homeostasis and the often harsh external environment. Cuticular hydrocarbons (CHCs) are key constituents of this hard cuticle and are associated with a variety of functions including stress response and communication. CHC production and d...
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doaj-a5822a06d49f4022b29864eefdee62592021-08-19T13:55:35ZengWileyEcology and Evolution2045-77582021-01-0111135236410.1002/ece3.7050Post‐eclosion temperature effects on insect cuticular hydrocarbon profilesSubhash Rajpurohit0Vladimír Vrkoslav1Robert Hanus2Allen G. Gibbs3Josef Cvačka4Paul S Schmidt5Division of Biological and Life Sciences School of Arts and Sciences Ahmedabad University Ahmedabad IndiaInstitute of Organic Chemistry and Biochemistry AS CR Prague Czech RepublicInstitute of Organic Chemistry and Biochemistry AS CR Prague Czech RepublicSchool of Life Sciences University of Nevada Las Vegas NV USAInstitute of Organic Chemistry and Biochemistry AS CR Prague Czech RepublicDepartment of Biology University of Pennsylvania Philadelphia PA USAAbstract The insect cuticle is the interface between internal homeostasis and the often harsh external environment. Cuticular hydrocarbons (CHCs) are key constituents of this hard cuticle and are associated with a variety of functions including stress response and communication. CHC production and deposition on the insect cuticle vary among natural populations and are affected by developmental temperature; however, little is known about CHC plasticity in response to the environment experienced following eclosion, during which time the insect cuticle undergoes several crucial changes. We targeted this crucial to important phase and studied post‐eclosion temperature effects on CHC profiles in two natural populations of Drosophila melanogaster. A forty‐eight hour post‐eclosion exposure to three different temperatures (18, 25, and 30°C) significantly affected CHCs in both ancestral African and more recently derived North American populations of D. melanogaster. A clear shift from shorter to longer CHCs chain length was observed with increasing temperature, and the effects of post‐eclosion temperature varied across populations and between sexes. The quantitative differences in CHCs were associated with variation in desiccation tolerance among populations. Surprisingly, we did not detect any significant differences in water loss rate between African and North American populations. Overall, our results demonstrate strong genetic and plasticity effects in CHC profiles in response to environmental temperatures experienced at the adult stage as well as associations with desiccation tolerance, which is crucial in understanding holometabolan responses to stress.https://doi.org/10.1002/ece3.7050cuticular hydrocarbonsdesiccation toleranceDrosophila melanogastereclosionnatural populationsphenotypic plasticity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Subhash Rajpurohit Vladimír Vrkoslav Robert Hanus Allen G. Gibbs Josef Cvačka Paul S Schmidt |
spellingShingle |
Subhash Rajpurohit Vladimír Vrkoslav Robert Hanus Allen G. Gibbs Josef Cvačka Paul S Schmidt Post‐eclosion temperature effects on insect cuticular hydrocarbon profiles Ecology and Evolution cuticular hydrocarbons desiccation tolerance Drosophila melanogaster eclosion natural populations phenotypic plasticity |
author_facet |
Subhash Rajpurohit Vladimír Vrkoslav Robert Hanus Allen G. Gibbs Josef Cvačka Paul S Schmidt |
author_sort |
Subhash Rajpurohit |
title |
Post‐eclosion temperature effects on insect cuticular hydrocarbon profiles |
title_short |
Post‐eclosion temperature effects on insect cuticular hydrocarbon profiles |
title_full |
Post‐eclosion temperature effects on insect cuticular hydrocarbon profiles |
title_fullStr |
Post‐eclosion temperature effects on insect cuticular hydrocarbon profiles |
title_full_unstemmed |
Post‐eclosion temperature effects on insect cuticular hydrocarbon profiles |
title_sort |
post‐eclosion temperature effects on insect cuticular hydrocarbon profiles |
publisher |
Wiley |
series |
Ecology and Evolution |
issn |
2045-7758 |
publishDate |
2021-01-01 |
description |
Abstract The insect cuticle is the interface between internal homeostasis and the often harsh external environment. Cuticular hydrocarbons (CHCs) are key constituents of this hard cuticle and are associated with a variety of functions including stress response and communication. CHC production and deposition on the insect cuticle vary among natural populations and are affected by developmental temperature; however, little is known about CHC plasticity in response to the environment experienced following eclosion, during which time the insect cuticle undergoes several crucial changes. We targeted this crucial to important phase and studied post‐eclosion temperature effects on CHC profiles in two natural populations of Drosophila melanogaster. A forty‐eight hour post‐eclosion exposure to three different temperatures (18, 25, and 30°C) significantly affected CHCs in both ancestral African and more recently derived North American populations of D. melanogaster. A clear shift from shorter to longer CHCs chain length was observed with increasing temperature, and the effects of post‐eclosion temperature varied across populations and between sexes. The quantitative differences in CHCs were associated with variation in desiccation tolerance among populations. Surprisingly, we did not detect any significant differences in water loss rate between African and North American populations. Overall, our results demonstrate strong genetic and plasticity effects in CHC profiles in response to environmental temperatures experienced at the adult stage as well as associations with desiccation tolerance, which is crucial in understanding holometabolan responses to stress. |
topic |
cuticular hydrocarbons desiccation tolerance Drosophila melanogaster eclosion natural populations phenotypic plasticity |
url |
https://doi.org/10.1002/ece3.7050 |
work_keys_str_mv |
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