| Summary: | Inflammation is a very common and important pathological process that can cause many diseases. The discovery of anti-inflammatory drugs and the treatment of inflammation are particularly essential. Dammarane-type triterpenoid saponins (PNS) were demonstrated to show anti-inflammatory effects in the leaves of <i>Panax notoginseng</i>. Chromatographies and spectral analysis methods were combined to isolate and identify PNS. Moreover, the nitric oxide (NO) inhibitory activities of all compounds were examined in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. As a result, eleven new dammarane-type triterpenoid saponins, notoginsenosides NL-A<sub>1</sub>−NL-A<sub>4</sub> (<b>1</b>−<b>4</b>), NL-B<sub>1</sub>−NL-B<sub>3</sub> (<b>5</b>−<b>7</b>), NL-C<sub>1</sub>−NL-C<sub>3</sub> (<b>8</b>−<b>10</b>), and NL-D (<b>11</b>) were isolated, and their structures were identified by using various spectrometric techniques and chemical reactions. Among them, compounds <b>4</b> and <b>11</b> were characterized by the malonyl substitution at 3-position. The 3-malonyl substituted dammarane-type terpennoids were first obtained from natural products. In addition, compounds <b>1</b>, <b>2</b>, <b>5</b>, <b>6</b>, and <b>8</b>−<b>10</b> were found to play an important role in suppressing NO levels at 50 μM, without cytotoxicity. All inhibitory activities were found to be dose-dependent.
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