Changes in ACTN3 gene expression and fiber type composition in flexor hallucis longus muscle after eight weeks progressive resistance training in Sprague-Dawley rats
Background: Alpha-actinins are located in the skeletal muscle Z-line and form actin–actin cross-links. It belongs to a highly conserved family of actin-binding proteins- the spectrin superfamily, which also contains the spectrins and dystrophin. Mammalian skeletal muscle has two isoforms:...
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Tehran University of Medical Sciences
2013-04-01
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doaj-a597b34144794dacb6900b3ef8c30bd92020-11-24T22:10:14ZfasTehran University of Medical SciencesTehran University Medical Journal1683-17641735-73222013-04-017113745Changes in ACTN3 gene expression and fiber type composition in flexor hallucis longus muscle after eight weeks progressive resistance training in Sprague-Dawley ratsAli Gaeini AKhaledi NFayazmilani RRavasi ASedghroohi GArabkari VBackground: Alpha-actinins are located in the skeletal muscle Z-line and form actin–actin cross-links. It belongs to a highly conserved family of actin-binding proteins- the spectrin superfamily, which also contains the spectrins and dystrophin. Mammalian skeletal muscle has two isoforms: alpha-actinins-2 and alpha-actinins-3. However, the response of alpha-actinins to exercise training is little understood. This study examined the effects of 8 weeks of resistance training on muscle mass, ACTN3 (alpha-actinins-3) gene expression levels and fiber type composition in the flexor hallucis longus (FHL) muscle.Methods: Forty five female Sprague-Dawley rats (Initial body mass: 169.25±9gr age: 3 month) were obtained and assigned to a control (C; n=18) or exercise training (T; n=22) and pilot (P; n=5) groups. The resistance training consisted of climbing a ladder carry-ing a load suspended from the tail and the weight increased progressively. Real-time PCR and Immunohistochemistry techniques were used to measure gene expression leve-ls and myosin heavy chain (MyHC) composition, respectively.Results: Following 8 weeks of training, we observed significant increase in absolute muscle mass in FHL (P=0.01). Results showed that no significant difference was found in ACTN3 gene expression levels between training and control groups (P=0.852 respecti-vely). Also, Pearson coefficient didn't indicated any significant relationships in gene expression and Fiber type IIX in response to resistance training in FHL (r=0.12).Conclusion: However, resistance training effects on sarcomeric proteins development, these results showed no effect of resistance training on alpha-actinins-3 levels. Althou-gh alpha-actinins-3 has an important function to produce and progress of force in sarco-mere, but didn't changed significantly in response to resistance training.http://tumj.tums.ac.ir/browse.php?a_code=A-10-25-36&slc_lang=en&sid=1Alpha-actininsflexor hallucis longusresistance trainingvertical ladderz-line |
collection |
DOAJ |
language |
fas |
format |
Article |
sources |
DOAJ |
author |
Ali Gaeini A Khaledi N Fayazmilani R Ravasi A Sedghroohi G Arabkari V |
spellingShingle |
Ali Gaeini A Khaledi N Fayazmilani R Ravasi A Sedghroohi G Arabkari V Changes in ACTN3 gene expression and fiber type composition in flexor hallucis longus muscle after eight weeks progressive resistance training in Sprague-Dawley rats Tehran University Medical Journal Alpha-actinins flexor hallucis longus resistance training vertical ladder z-line |
author_facet |
Ali Gaeini A Khaledi N Fayazmilani R Ravasi A Sedghroohi G Arabkari V |
author_sort |
Ali Gaeini A |
title |
Changes in ACTN3 gene expression and fiber type composition in flexor hallucis longus muscle after eight weeks progressive resistance training in Sprague-Dawley rats |
title_short |
Changes in ACTN3 gene expression and fiber type composition in flexor hallucis longus muscle after eight weeks progressive resistance training in Sprague-Dawley rats |
title_full |
Changes in ACTN3 gene expression and fiber type composition in flexor hallucis longus muscle after eight weeks progressive resistance training in Sprague-Dawley rats |
title_fullStr |
Changes in ACTN3 gene expression and fiber type composition in flexor hallucis longus muscle after eight weeks progressive resistance training in Sprague-Dawley rats |
title_full_unstemmed |
Changes in ACTN3 gene expression and fiber type composition in flexor hallucis longus muscle after eight weeks progressive resistance training in Sprague-Dawley rats |
title_sort |
changes in actn3 gene expression and fiber type composition in flexor hallucis longus muscle after eight weeks progressive resistance training in sprague-dawley rats |
publisher |
Tehran University of Medical Sciences |
series |
Tehran University Medical Journal |
issn |
1683-1764 1735-7322 |
publishDate |
2013-04-01 |
description |
Background: Alpha-actinins are located in the skeletal muscle Z-line and form actin–actin cross-links. It belongs to a highly conserved family of actin-binding proteins- the spectrin superfamily, which also contains the spectrins and dystrophin. Mammalian skeletal muscle has two isoforms: alpha-actinins-2 and alpha-actinins-3. However, the response of alpha-actinins to exercise training is little understood. This study examined the effects of 8 weeks of resistance training on muscle mass, ACTN3 (alpha-actinins-3) gene expression levels and fiber type composition in the flexor hallucis longus (FHL) muscle.Methods: Forty five female Sprague-Dawley rats (Initial body mass: 169.25±9gr age: 3 month) were obtained and assigned to a control (C; n=18) or exercise training (T; n=22) and pilot (P; n=5) groups. The resistance training consisted of climbing a ladder carry-ing a load suspended from the tail and the weight increased progressively. Real-time PCR and Immunohistochemistry techniques were used to measure gene expression leve-ls and myosin heavy chain (MyHC) composition, respectively.Results: Following 8 weeks of training, we observed significant increase in absolute muscle mass in FHL (P=0.01). Results showed that no significant difference was found in ACTN3 gene expression levels between training and control groups (P=0.852 respecti-vely). Also, Pearson coefficient didn't indicated any significant relationships in gene expression and Fiber type IIX in response to resistance training in FHL (r=0.12).Conclusion: However, resistance training effects on sarcomeric proteins development, these results showed no effect of resistance training on alpha-actinins-3 levels. Althou-gh alpha-actinins-3 has an important function to produce and progress of force in sarco-mere, but didn't changed significantly in response to resistance training. |
topic |
Alpha-actinins flexor hallucis longus resistance training vertical ladder z-line |
url |
http://tumj.tums.ac.ir/browse.php?a_code=A-10-25-36&slc_lang=en&sid=1 |
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