Cell Density Detector Based on Light Beam Focusing
Although the lab-on-a-chip system has been successfully applied in a wide variety of fields, the goal of achieving a cell counter with simple operation, low cost, and high accuracy still attracts continuous research efforts. In this paper, the authors explore a cell counter based on light beam focus...
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doaj-a5a1e460007845ecbe24b607bbd2e7052020-11-24T21:48:18ZengMDPI AGMicromachines2072-666X2018-11-0191159210.3390/mi9110592mi9110592Cell Density Detector Based on Light Beam FocusingAoqun Jian0Huiming Li1Yixia Zhang2Qianqian Duan3Qianwu Zhang4Shengbo Sang5MicroNano System Research Center, College of Information Engineering and Computer Science, Taiyuan University of Technology, Jinzhong 030600, ChinaMicroNano System Research Center, College of Information Engineering and Computer Science, Taiyuan University of Technology, Jinzhong 030600, ChinaInstitute of Applied Mechanics and Biomedical Engineering & National Demonstration Center for Experimental Mechanics Education, College of Mechanics, Taiyuan University of Technology, Taiyuan 030024, ChinaMicroNano System Research Center, College of Information Engineering and Computer Science, Taiyuan University of Technology, Jinzhong 030600, ChinaKey Lab Specialty Fiber Opt & Opt Access Networks, Shanghai University, Shanghai 200072, ChinaMicroNano System Research Center, College of Information Engineering and Computer Science, Taiyuan University of Technology, Jinzhong 030600, ChinaAlthough the lab-on-a-chip system has been successfully applied in a wide variety of fields, the goal of achieving a cell counter with simple operation, low cost, and high accuracy still attracts continuous research efforts. In this paper, the authors explore a cell counter based on light beam focusing to measure the density of adherent cells. In this sensor, the light emitted from the optical fibers is collimated by the collimating lens formed in polydimethylsiloxane (PDMS). The uniformly attached adherent cells act as a convex lens, focusing the collimated light propagated through them. The intensity of the focused light indicates the density of the adherent cells. For Hela cells, a detection limit of 8.3 × 10<sup>4</sup> cells/mL with a detection range from 0.1 × 10<sup>6</sup> cells/mL to 1.0 × 10<sup>6</sup> cells/mL is achieved. This sensor is particularly useful for drug screening, cell pathology analysis, and cancer pre-diagnosis.https://www.mdpi.com/2072-666X/9/11/592cell countingmicrofluidics systemlight beam focusing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Aoqun Jian Huiming Li Yixia Zhang Qianqian Duan Qianwu Zhang Shengbo Sang |
spellingShingle |
Aoqun Jian Huiming Li Yixia Zhang Qianqian Duan Qianwu Zhang Shengbo Sang Cell Density Detector Based on Light Beam Focusing Micromachines cell counting microfluidics system light beam focusing |
author_facet |
Aoqun Jian Huiming Li Yixia Zhang Qianqian Duan Qianwu Zhang Shengbo Sang |
author_sort |
Aoqun Jian |
title |
Cell Density Detector Based on Light Beam Focusing |
title_short |
Cell Density Detector Based on Light Beam Focusing |
title_full |
Cell Density Detector Based on Light Beam Focusing |
title_fullStr |
Cell Density Detector Based on Light Beam Focusing |
title_full_unstemmed |
Cell Density Detector Based on Light Beam Focusing |
title_sort |
cell density detector based on light beam focusing |
publisher |
MDPI AG |
series |
Micromachines |
issn |
2072-666X |
publishDate |
2018-11-01 |
description |
Although the lab-on-a-chip system has been successfully applied in a wide variety of fields, the goal of achieving a cell counter with simple operation, low cost, and high accuracy still attracts continuous research efforts. In this paper, the authors explore a cell counter based on light beam focusing to measure the density of adherent cells. In this sensor, the light emitted from the optical fibers is collimated by the collimating lens formed in polydimethylsiloxane (PDMS). The uniformly attached adherent cells act as a convex lens, focusing the collimated light propagated through them. The intensity of the focused light indicates the density of the adherent cells. For Hela cells, a detection limit of 8.3 × 10<sup>4</sup> cells/mL with a detection range from 0.1 × 10<sup>6</sup> cells/mL to 1.0 × 10<sup>6</sup> cells/mL is achieved. This sensor is particularly useful for drug screening, cell pathology analysis, and cancer pre-diagnosis. |
topic |
cell counting microfluidics system light beam focusing |
url |
https://www.mdpi.com/2072-666X/9/11/592 |
work_keys_str_mv |
AT aoqunjian celldensitydetectorbasedonlightbeamfocusing AT huimingli celldensitydetectorbasedonlightbeamfocusing AT yixiazhang celldensitydetectorbasedonlightbeamfocusing AT qianqianduan celldensitydetectorbasedonlightbeamfocusing AT qianwuzhang celldensitydetectorbasedonlightbeamfocusing AT shengbosang celldensitydetectorbasedonlightbeamfocusing |
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1725892994035351552 |