| Summary: | The study aims to develop novel biofungicide formulations against rice blast disease. A total of 300 rhizobacteria strains were isolated from rice field soil and were examined for the inhibition of <i>Magnaporthe oryzae</i> growth in a vitro test. Among them, only six rhizobacteria showed inhibition against <i>M. oryzae</i>. The three strains that showed the highest inhibition were <i>Bacillus subtilis</i> 5, <i>B. cereus</i> 3S5, and <i>Pseudomonas fluorecens</i> 10S2. A rice hull mixture and liquid medium were mixed with the above-mentioned bacterial suspensions into three bacterial formulas and tested separately on the rice cultivar UPLRi-5 after infection by <i>M. oryzae</i> under a controlled condition. The three novel biofungicide formulas significantly inhibited rice blast disease intensity with a mean disease control rate being approximately 31% higher than the control. The formulas proved to be effective and should be considered as promising novel treatments for rice blast disease.
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