Conditioned medium-electrospun fiber biomaterials for skin regeneration

• Main Authors: Lu Chen, Liying Cheng, Zhen Wang, Jianming Zhang, Xiyuan Mao, Zhimo Liu, Yuguang Zhang, Wenguo Cui, Xiaoming Sun
• Format: Article
• Language: English
• Published: KeAi Communications Co., Ltd. 2021-02-01
• Series: Bioactive Materials
• Subjects: Conditioned medium; Biomaterials; Regenerative medicine; Skin regeneration; Electrospun fiber
• Online Access: http://www.sciencedirect.com/science/article/pii/S2452199X20301900

Conditioned medium (CM) contains variety of factors secreted by cells, which directly regulate cellular processes, showing tremendous potential in regenerative medicine. Here, for the first time, we proposed a novel regenerative therapy mediated by biodegradable micro-nano electrospun fibers loaded with highly active conditioned medium of adipose-derived stem cells (ADSC-CM). ADSC-CM was successfully loaded into the nanofibers with biological protection and controllable sustained-release properties by emulsion electrospinning and protein freeze-drying technologies. In vitro, ADSC-CM released by the fibers accelerated the migration rate of fibroblasts; inhibited the over proliferation of fibroblasts by inducing apoptosis and damaging cell membrane; in addition, ADSC-CM inhibited the transformation of fibroblasts into myofibroblasts and suppressed excessive production of extracellular matrix (ECM). In vivo, the application of CM-biomaterials significantly accelerated wound closure and improved regeneration outcome, showing superior pro-regenerative performance. This study pioneered the application of CM-biomaterials in regenerative medicine, and confirmed the practicability and significant biological effects of this innovative biomaterials.