Characterization of Osteogenesis and Chondrogenesis of Human Decellularized Allogeneic Bone with Mesenchymal Stem Cells Derived from Bone Marrow, Adipose Tissue, and Wharton’s Jelly
Allogeneic bone grafts are a promising material for bone implantation due to reduced operative trauma, reduced blood loss, and no donor-site morbidity. Although human decellularized allogeneic bone (hDCB) can be used to fill bone defects, the research of revitalizing hDCB blocks with human mesenchym...
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doaj-a5c820fdbd454576a120f8fd2f4149d32021-08-26T13:53:32ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-01228987898710.3390/ijms22168987Characterization of Osteogenesis and Chondrogenesis of Human Decellularized Allogeneic Bone with Mesenchymal Stem Cells Derived from Bone Marrow, Adipose Tissue, and Wharton’s JellyCheng-Fong Chen0Yi-Chun Chen1Yu-Show Fu2Shang-Wen Tsai3Po-Kuei Wu4Chao-Ming Chen5Ming-Chau Chang6Wei-Ming Chen7Department of Orthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei 11217, TaiwanDepartment of Orthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei 11217, TaiwanDepartment of Anatomy and Cell Biology, Faculty of Medicine, National Yang Ming Chiao Tung University, Taipei 11221, TaiwanDepartment of Orthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei 11217, TaiwanDepartment of Orthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei 11217, TaiwanDepartment of Orthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei 11217, TaiwanDepartment of Orthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei 11217, TaiwanDepartment of Orthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei 11217, TaiwanAllogeneic bone grafts are a promising material for bone implantation due to reduced operative trauma, reduced blood loss, and no donor-site morbidity. Although human decellularized allogeneic bone (hDCB) can be used to fill bone defects, the research of revitalizing hDCB blocks with human mesenchymal stem cells (hMSCs) for osteochondral regeneration is missing. The hMSCs derived from bone marrow, adipose tissue, and Wharton’s jelly (BMMSCs, ADMSCs, and UMSCs, respectively) are potential candidates for bone regeneration. This study characterized the potential of hDCB as a scaffold for osteogenesis and chondrogenesis of BMMSCs, ADMSCs, and UMSCs. The pore sizes and mechanical strength of hDCB were characterized. Cell survival and adhesion of hMSCs were investigated using MTT assay and F-actin staining. Alizarin Red S and Safranin O staining were conducted to demonstrate calcium deposition and proteoglycan production of hMSCs after osteogenic and chondrogenic differentiation, respectively. A RT-qPCR was performed to analyze the expression levels of osteogenic and chondrogenic markers in hMSCs. Results indicated that BMMSCs and ADMSCs exhibited higher osteogenic potential than UMSCs. Furthermore, ADMSCs and UMSCs had higher chondrogenic potential than BMMSCs. This study demonstrated that chondrogenic ADMSCs- or UMSCs-seeded hDCB might be potential osteochondral constructs for osteochondral regeneration.https://www.mdpi.com/1422-0067/22/16/8987MSCosteogenesischondrogenesisallogeneic bone |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cheng-Fong Chen Yi-Chun Chen Yu-Show Fu Shang-Wen Tsai Po-Kuei Wu Chao-Ming Chen Ming-Chau Chang Wei-Ming Chen |
spellingShingle |
Cheng-Fong Chen Yi-Chun Chen Yu-Show Fu Shang-Wen Tsai Po-Kuei Wu Chao-Ming Chen Ming-Chau Chang Wei-Ming Chen Characterization of Osteogenesis and Chondrogenesis of Human Decellularized Allogeneic Bone with Mesenchymal Stem Cells Derived from Bone Marrow, Adipose Tissue, and Wharton’s Jelly International Journal of Molecular Sciences MSC osteogenesis chondrogenesis allogeneic bone |
author_facet |
Cheng-Fong Chen Yi-Chun Chen Yu-Show Fu Shang-Wen Tsai Po-Kuei Wu Chao-Ming Chen Ming-Chau Chang Wei-Ming Chen |
author_sort |
Cheng-Fong Chen |
title |
Characterization of Osteogenesis and Chondrogenesis of Human Decellularized Allogeneic Bone with Mesenchymal Stem Cells Derived from Bone Marrow, Adipose Tissue, and Wharton’s Jelly |
title_short |
Characterization of Osteogenesis and Chondrogenesis of Human Decellularized Allogeneic Bone with Mesenchymal Stem Cells Derived from Bone Marrow, Adipose Tissue, and Wharton’s Jelly |
title_full |
Characterization of Osteogenesis and Chondrogenesis of Human Decellularized Allogeneic Bone with Mesenchymal Stem Cells Derived from Bone Marrow, Adipose Tissue, and Wharton’s Jelly |
title_fullStr |
Characterization of Osteogenesis and Chondrogenesis of Human Decellularized Allogeneic Bone with Mesenchymal Stem Cells Derived from Bone Marrow, Adipose Tissue, and Wharton’s Jelly |
title_full_unstemmed |
Characterization of Osteogenesis and Chondrogenesis of Human Decellularized Allogeneic Bone with Mesenchymal Stem Cells Derived from Bone Marrow, Adipose Tissue, and Wharton’s Jelly |
title_sort |
characterization of osteogenesis and chondrogenesis of human decellularized allogeneic bone with mesenchymal stem cells derived from bone marrow, adipose tissue, and wharton’s jelly |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-08-01 |
description |
Allogeneic bone grafts are a promising material for bone implantation due to reduced operative trauma, reduced blood loss, and no donor-site morbidity. Although human decellularized allogeneic bone (hDCB) can be used to fill bone defects, the research of revitalizing hDCB blocks with human mesenchymal stem cells (hMSCs) for osteochondral regeneration is missing. The hMSCs derived from bone marrow, adipose tissue, and Wharton’s jelly (BMMSCs, ADMSCs, and UMSCs, respectively) are potential candidates for bone regeneration. This study characterized the potential of hDCB as a scaffold for osteogenesis and chondrogenesis of BMMSCs, ADMSCs, and UMSCs. The pore sizes and mechanical strength of hDCB were characterized. Cell survival and adhesion of hMSCs were investigated using MTT assay and F-actin staining. Alizarin Red S and Safranin O staining were conducted to demonstrate calcium deposition and proteoglycan production of hMSCs after osteogenic and chondrogenic differentiation, respectively. A RT-qPCR was performed to analyze the expression levels of osteogenic and chondrogenic markers in hMSCs. Results indicated that BMMSCs and ADMSCs exhibited higher osteogenic potential than UMSCs. Furthermore, ADMSCs and UMSCs had higher chondrogenic potential than BMMSCs. This study demonstrated that chondrogenic ADMSCs- or UMSCs-seeded hDCB might be potential osteochondral constructs for osteochondral regeneration. |
topic |
MSC osteogenesis chondrogenesis allogeneic bone |
url |
https://www.mdpi.com/1422-0067/22/16/8987 |
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