Effect of explant type on the rooting and acclimatization of Dianthus serotinus Waldst. & Kit.
The effect of the concentration of MS salts and explant type on D. serotinus rooting and acclimatization was investigated in order to optimize a protocol for the micropropagation of this species. The obtained results showed that explant type as well as the concentration of MS salts had a si...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | srp |
| Published: |
Faculty of Forestry, Belgrade
2014-01-01
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| Series: | Glasnik Šumarskog Fakulteta: Univerzitet u Beogradu |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0353-4537/2014/0353-45371409125M.pdf |
| Summary: | The effect of the concentration of MS salts and explant type on D. serotinus
rooting and acclimatization was investigated in order to optimize a protocol
for the micropropagation of this species. The obtained results showed that
explant type as well as the concentration of MS salts had a significant
effect on rooting, and the highest rooting rate (85-86,7%) was achieved when
culturing single-node cuttings and terminal buds on a half-strength MS medium
supplemented with 0,5 mgL-1 NAA. Nevertheless, mean number of roots per
explant was higher on the MS media (15,3-18,6) than on the half-strength MS
media (11,8-13,4). The best acclimatization rate was obtained in a 4:1
mixture of peat and sand (83,3-86,7%). The explant type from which in vitro
plantlets developed had no effect on the acclimatization rate. [Projekat
Ministarstva nauke Republike Srbije, br. 43007] |
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| ISSN: | 0353-4537 2217-8600 |