Biochemical characterization of a recombinant acid phosphatase from Acinetobacter baumannii.

Biochemical characterization of a recombinant acid phosphatase from Acinetobacter baumannii.

Genomic sequence analysis of Acinetobacter baumannii revealed the presence of a putative Acid Phosphatase (AcpA; EC 3.1.3.2). A plasmid construct was made, and recombinant protein (rAcpA) was expressed in E. coli. PAGE analysis (carried out under denaturing/reducing conditions) of nickel-affinity pu...

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Bibliographic Details
Main Authors: Elizabeth Smiley-Moreno, Douglas Smith, Jieh-Juen Yu, Phuong Cao, Bernard P Arulanandam, James P Chambers
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2021-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0252377

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