Angiotensin-converting enzyme insertion/deletion gene polymorphism is associated with dermatomyositis
Background and objective: The cornerstone of dermatomyositis (DM) pathogenesis involves vascular disturbance that leads to hypoxia, capillary necrosis and muscle perifascicular atrophy. Hence, the hypothesis is that the angiotensin-converting enzyme (ACE) insertion/deletion (I/D) gene polymorphism c...
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2015-09-01
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Series: | Journal of the Renin-Angiotensin-Aldosterone System |
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doaj-a5e221a62218411a9f5820ee4122df482021-05-02T19:10:40ZengHindawi - SAGE PublishingJournal of the Renin-Angiotensin-Aldosterone System1470-32031752-89762015-09-011610.1177/1470320314524995Angiotensin-converting enzyme insertion/deletion gene polymorphism is associated with dermatomyositisSamuel K Shinjo0Miyuki Uno1Sueli M Oba-Shinjo2Suely KN Marie3Division of Rheumatology, Universidade de São Paulo, BrazilDepartment of Neurology, Universidade de São Paulo, BrazilDepartment of Neurology, Universidade de São Paulo, BrazilDepartment of Neurology, Universidade de São Paulo, BrazilBackground and objective: The cornerstone of dermatomyositis (DM) pathogenesis involves vascular disturbance that leads to hypoxia, capillary necrosis and muscle perifascicular atrophy. Hence, the hypothesis is that the angiotensin-converting enzyme (ACE) insertion/deletion (I/D) gene polymorphism could be associated with susceptibility to DM. Method: A single centre, case control study that genotyped ACE gene in 88 DM and 99 healthy individuals. The ACE gene polymorphism was determined by melting curve analysis of real-time polymerase chain reaction products using SYBR Green. Results: The DM and the control subjects had a comparable mean age, gender frequency and ethnicity. The frequency of the D allele was higher in DM than in the control individuals (63.6% vs 55.6%, respectively). The DM had more ACE D/D and less ACE I/D genotype when compared to the control individuals, whereas the ACE I/I genotype distribution was similar in both case and control groups. Moreover, after sex-age-adjusted analysis, the ACE D/D genotype was strongly associated with DM disease (odds ratio (OR) 2.44, 95% confidence interval (CI): 1.17–4.37), in contrast to ACE I/D genotype (OR 0.51, 95% CI: 0.28–0.93). Conclusions: Homozygous ACE D/D was associated significantly with the DM risk. Further investigations are required to clarify and to confirm the association of these genes with DM susceptibility.https://doi.org/10.1177/1470320314524995 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Samuel K Shinjo Miyuki Uno Sueli M Oba-Shinjo Suely KN Marie |
spellingShingle |
Samuel K Shinjo Miyuki Uno Sueli M Oba-Shinjo Suely KN Marie Angiotensin-converting enzyme insertion/deletion gene polymorphism is associated with dermatomyositis Journal of the Renin-Angiotensin-Aldosterone System |
author_facet |
Samuel K Shinjo Miyuki Uno Sueli M Oba-Shinjo Suely KN Marie |
author_sort |
Samuel K Shinjo |
title |
Angiotensin-converting enzyme insertion/deletion gene polymorphism is associated with dermatomyositis |
title_short |
Angiotensin-converting enzyme insertion/deletion gene polymorphism is associated with dermatomyositis |
title_full |
Angiotensin-converting enzyme insertion/deletion gene polymorphism is associated with dermatomyositis |
title_fullStr |
Angiotensin-converting enzyme insertion/deletion gene polymorphism is associated with dermatomyositis |
title_full_unstemmed |
Angiotensin-converting enzyme insertion/deletion gene polymorphism is associated with dermatomyositis |
title_sort |
angiotensin-converting enzyme insertion/deletion gene polymorphism is associated with dermatomyositis |
publisher |
Hindawi - SAGE Publishing |
series |
Journal of the Renin-Angiotensin-Aldosterone System |
issn |
1470-3203 1752-8976 |
publishDate |
2015-09-01 |
description |
Background and objective: The cornerstone of dermatomyositis (DM) pathogenesis involves vascular disturbance that leads to hypoxia, capillary necrosis and muscle perifascicular atrophy. Hence, the hypothesis is that the angiotensin-converting enzyme (ACE) insertion/deletion (I/D) gene polymorphism could be associated with susceptibility to DM. Method: A single centre, case control study that genotyped ACE gene in 88 DM and 99 healthy individuals. The ACE gene polymorphism was determined by melting curve analysis of real-time polymerase chain reaction products using SYBR Green. Results: The DM and the control subjects had a comparable mean age, gender frequency and ethnicity. The frequency of the D allele was higher in DM than in the control individuals (63.6% vs 55.6%, respectively). The DM had more ACE D/D and less ACE I/D genotype when compared to the control individuals, whereas the ACE I/I genotype distribution was similar in both case and control groups. Moreover, after sex-age-adjusted analysis, the ACE D/D genotype was strongly associated with DM disease (odds ratio (OR) 2.44, 95% confidence interval (CI): 1.17–4.37), in contrast to ACE I/D genotype (OR 0.51, 95% CI: 0.28–0.93). Conclusions: Homozygous ACE D/D was associated significantly with the DM risk. Further investigations are required to clarify and to confirm the association of these genes with DM susceptibility. |
url |
https://doi.org/10.1177/1470320314524995 |
work_keys_str_mv |
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