Photoluminescent Cationic Carbon Dots as efficient Non-Viral Delivery of Plasmid SOX9 and Chondrogenesis of Fibroblasts
Abstract With the increasing demand for higher gene carrier performance, a multifunctional vector could immensely simplify gene delivery for disease treatment; nevertheless, the current non- viral vectors lack self-tracking ability. Here, a type of novel, dual-functional cationic carbon dots (CDs),...
Main Authors: | , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2018-05-01
|
Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-018-25330-x |
id |
doaj-a5eade4c344243929a4ad2aafa674839 |
---|---|
record_format |
Article |
spelling |
doaj-a5eade4c344243929a4ad2aafa6748392020-12-08T03:42:30ZengNature Publishing GroupScientific Reports2045-23222018-05-018111110.1038/s41598-018-25330-xPhotoluminescent Cationic Carbon Dots as efficient Non-Viral Delivery of Plasmid SOX9 and Chondrogenesis of FibroblastsXia Cao0Jianping Wang1Wenwen Deng2Jingjing Chen3Yan Wang4Jie Zhou5Pan Du6Wenqian Xu7Qiang Wang8Qilong Wang9Qingtong Yu10Myron Spector11Jiangnan Yu12Ximing Xu13Department of Pharmaceutics, School of Pharmacy, and Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu UniversityDepartment of Pharmaceutics, School of Pharmacy, and Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu UniversityDepartment of Pharmaceutics, School of Pharmacy, and Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu UniversityDepartment of Pharmaceutics, School of Pharmacy, and Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu UniversityDepartment of Pharmaceutics, School of Pharmacy, and Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu UniversityDepartment of Pharmaceutics, School of Pharmacy, and Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu UniversityDepartment of Pharmaceutics, School of Pharmacy, and Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu UniversityDepartment of Pharmaceutics, School of Pharmacy, and Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu UniversityDepartment of Pharmaceutics, School of Pharmacy, and Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu UniversityDepartment of Pharmaceutics, School of Pharmacy, and Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu UniversityDepartment of Pharmaceutics, School of Pharmacy, and Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu UniversityDepartment of Orthopedic Surgery, Harvard Medical School, Brigham and Women’s HospitalDepartment of Pharmaceutics, School of Pharmacy, and Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu UniversityDepartment of Pharmaceutics, School of Pharmacy, and Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu UniversityAbstract With the increasing demand for higher gene carrier performance, a multifunctional vector could immensely simplify gene delivery for disease treatment; nevertheless, the current non- viral vectors lack self-tracking ability. Here, a type of novel, dual-functional cationic carbon dots (CDs), produced through one-step, microwave-assisted pyrolysis of arginine and glucose, have been utilized as both a self-imaging agent and a non-viral gene vector for chondrogenesis from fibroblasts. The cationic CDs could condense the model gene plasmid SOX9 (pSOX9) to form ultra-small (10–30 nm) nanoparticles which possessed several favorable properties, including high solubility, tunable fluorescence, high yield, low cytotoxicity and outstanding biocompatibility. The MTT assay indicated that CDs/pSOX9 nanoparticles had little cytotoxicity against mouse embryonic fibroblasts (MEFs) compared to Lipofectamine2000 and PEI (25 kDa). Importantly, the CDs/pSOX9 nanoparticles with tunable fluorescence not only enabled the intracellular tracking of the nanoparticles, but also could successfully deliver the pSOX9 into MEFs with significantly high efficiency. Furthermore, the CDs/pSOX9 nanoparticles-mediated transfection of MEFs showed obvious chondrogenic differentiation. Altogether, these findings demonstrated that the CDs prepared in this study could serve as a paradigmatic example of the dual-functional reagent for both self-imaging and effective non-viral gene delivery.https://doi.org/10.1038/s41598-018-25330-x |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xia Cao Jianping Wang Wenwen Deng Jingjing Chen Yan Wang Jie Zhou Pan Du Wenqian Xu Qiang Wang Qilong Wang Qingtong Yu Myron Spector Jiangnan Yu Ximing Xu |
spellingShingle |
Xia Cao Jianping Wang Wenwen Deng Jingjing Chen Yan Wang Jie Zhou Pan Du Wenqian Xu Qiang Wang Qilong Wang Qingtong Yu Myron Spector Jiangnan Yu Ximing Xu Photoluminescent Cationic Carbon Dots as efficient Non-Viral Delivery of Plasmid SOX9 and Chondrogenesis of Fibroblasts Scientific Reports |
author_facet |
Xia Cao Jianping Wang Wenwen Deng Jingjing Chen Yan Wang Jie Zhou Pan Du Wenqian Xu Qiang Wang Qilong Wang Qingtong Yu Myron Spector Jiangnan Yu Ximing Xu |
author_sort |
Xia Cao |
title |
Photoluminescent Cationic Carbon Dots as efficient Non-Viral Delivery of Plasmid SOX9 and Chondrogenesis of Fibroblasts |
title_short |
Photoluminescent Cationic Carbon Dots as efficient Non-Viral Delivery of Plasmid SOX9 and Chondrogenesis of Fibroblasts |
title_full |
Photoluminescent Cationic Carbon Dots as efficient Non-Viral Delivery of Plasmid SOX9 and Chondrogenesis of Fibroblasts |
title_fullStr |
Photoluminescent Cationic Carbon Dots as efficient Non-Viral Delivery of Plasmid SOX9 and Chondrogenesis of Fibroblasts |
title_full_unstemmed |
Photoluminescent Cationic Carbon Dots as efficient Non-Viral Delivery of Plasmid SOX9 and Chondrogenesis of Fibroblasts |
title_sort |
photoluminescent cationic carbon dots as efficient non-viral delivery of plasmid sox9 and chondrogenesis of fibroblasts |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2018-05-01 |
description |
Abstract With the increasing demand for higher gene carrier performance, a multifunctional vector could immensely simplify gene delivery for disease treatment; nevertheless, the current non- viral vectors lack self-tracking ability. Here, a type of novel, dual-functional cationic carbon dots (CDs), produced through one-step, microwave-assisted pyrolysis of arginine and glucose, have been utilized as both a self-imaging agent and a non-viral gene vector for chondrogenesis from fibroblasts. The cationic CDs could condense the model gene plasmid SOX9 (pSOX9) to form ultra-small (10–30 nm) nanoparticles which possessed several favorable properties, including high solubility, tunable fluorescence, high yield, low cytotoxicity and outstanding biocompatibility. The MTT assay indicated that CDs/pSOX9 nanoparticles had little cytotoxicity against mouse embryonic fibroblasts (MEFs) compared to Lipofectamine2000 and PEI (25 kDa). Importantly, the CDs/pSOX9 nanoparticles with tunable fluorescence not only enabled the intracellular tracking of the nanoparticles, but also could successfully deliver the pSOX9 into MEFs with significantly high efficiency. Furthermore, the CDs/pSOX9 nanoparticles-mediated transfection of MEFs showed obvious chondrogenic differentiation. Altogether, these findings demonstrated that the CDs prepared in this study could serve as a paradigmatic example of the dual-functional reagent for both self-imaging and effective non-viral gene delivery. |
url |
https://doi.org/10.1038/s41598-018-25330-x |
work_keys_str_mv |
AT xiacao photoluminescentcationiccarbondotsasefficientnonviraldeliveryofplasmidsox9andchondrogenesisoffibroblasts AT jianpingwang photoluminescentcationiccarbondotsasefficientnonviraldeliveryofplasmidsox9andchondrogenesisoffibroblasts AT wenwendeng photoluminescentcationiccarbondotsasefficientnonviraldeliveryofplasmidsox9andchondrogenesisoffibroblasts AT jingjingchen photoluminescentcationiccarbondotsasefficientnonviraldeliveryofplasmidsox9andchondrogenesisoffibroblasts AT yanwang photoluminescentcationiccarbondotsasefficientnonviraldeliveryofplasmidsox9andchondrogenesisoffibroblasts AT jiezhou photoluminescentcationiccarbondotsasefficientnonviraldeliveryofplasmidsox9andchondrogenesisoffibroblasts AT pandu photoluminescentcationiccarbondotsasefficientnonviraldeliveryofplasmidsox9andchondrogenesisoffibroblasts AT wenqianxu photoluminescentcationiccarbondotsasefficientnonviraldeliveryofplasmidsox9andchondrogenesisoffibroblasts AT qiangwang photoluminescentcationiccarbondotsasefficientnonviraldeliveryofplasmidsox9andchondrogenesisoffibroblasts AT qilongwang photoluminescentcationiccarbondotsasefficientnonviraldeliveryofplasmidsox9andchondrogenesisoffibroblasts AT qingtongyu photoluminescentcationiccarbondotsasefficientnonviraldeliveryofplasmidsox9andchondrogenesisoffibroblasts AT myronspector photoluminescentcationiccarbondotsasefficientnonviraldeliveryofplasmidsox9andchondrogenesisoffibroblasts AT jiangnanyu photoluminescentcationiccarbondotsasefficientnonviraldeliveryofplasmidsox9andchondrogenesisoffibroblasts AT ximingxu photoluminescentcationiccarbondotsasefficientnonviraldeliveryofplasmidsox9andchondrogenesisoffibroblasts |
_version_ |
1724392405761785856 |