Transcriptomic and Functional Studies of the RGS Protein Rax1 in <i>Aspergillus fumigatus</i>

Transcriptomic and Functional Studies of the RGS Protein Rax1 in <i>Aspergillus fumigatus</i>

In the comparative transcriptomic studies of wild type (WT) and <i>rax1</i> null mutant strains, we obtained an average of 22,222,727 reads of 101 bp per sample and found that 183 genes showed greater than 2.0-fold differential expression, where 92 and 91 genes were up-and down-regulated...

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Bibliographic Details
Main Authors: Yong-Ho Choi, Min-Woo Lee, Olumuyiwa Ayokunle Igbalajobi, Jae-Hyuk Yu, Kwang-Soo Shin
Format: Article
Language:English
Published: MDPI AG 2019-12-01
Series:Pathogens
Subjects:
<i>aspergillus fumigatus</i>
glim
metacaspase
rax1
rna-seq
Online Access:https://www.mdpi.com/2076-0817/9/1/36
Description
Summary:In the comparative transcriptomic studies of wild type (WT) and <i>rax1</i> null mutant strains, we obtained an average of 22,222,727 reads of 101 bp per sample and found that 183 genes showed greater than 2.0-fold differential expression, where 92 and 91 genes were up-and down-regulated in &#916;<i>rax1</i> compared to WT, respectively. In accordance with the significantly reduced levels of <i>gliM</i> and <i>casB</i> transcripts in the absence of <i>rax1</i>, the &#916;<i>rax1</i> mutant exhibited increased sensitivity to exogenous gliotoxin (GT) without affecting levels of GT production. Moreover, &#916;<i>rax1</i> resulted in significantly restricted colony growth and reduced viability under endoplasmic reticulum stress condition. In summary, Rax1 positively affects expression of <i>gliM</i> and metacaspase genes.
ISSN:2076-0817

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