Effects on vesicular transport pathways at the late endosome in cells with limited very long-chain fatty acids[S]
Very long-chain fatty acids (VLCFAs), fatty acids with chain-length greater than 20 carbons, possess a wide range of biological functions. However, their roles at the molecular level remain largely unknown. In the present study, we screened for multicopy suppressors that rescued temperature-sensitiv...
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doaj-a60b16bb25e84a328fc1ab551ff5768a2021-04-28T06:05:40ZengElsevierJournal of Lipid Research0022-22752013-03-01543831842Effects on vesicular transport pathways at the late endosome in cells with limited very long-chain fatty acids[S]Keisuke Obara0Ryo Kojima1Akio Kihara2Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo 060-0812, JapanFaculty of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo 060-0812, JapanTo whom correspondence should be addressed. e-mail: kihara@pharm.hokudai.ac.jp.; To whom correspondence should be addressed. e-mail: kihara@pharm.hokudai.ac.jp.; Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo 060-0812, JapanVery long-chain fatty acids (VLCFAs), fatty acids with chain-length greater than 20 carbons, possess a wide range of biological functions. However, their roles at the molecular level remain largely unknown. In the present study, we screened for multicopy suppressors that rescued temperature-sensitive growth of VLCFA-limited yeast cells, and we identified the VPS21 gene, encoding a Rab GTPase, as such a suppressor. When the vps21Δ mutation was introduced into a deletion mutant of the SUR4 gene, which encodes a VLCFA elongase, a synthetic growth defect was observed. Endosome-mediated vesicular trafficking pathways, including endocytosis and the carboxypeptidase Y (CPY) pathway, were severely impaired in sur4Δ vps21Δ double mutants, while the AP-3 pathway that bypasses the endosome was unaffected. In addition, the sur4Δ mutant also exhibited a synthetic growth defect when combined with the deletion of VPS3, which encodes a subunit of the class C core vacuole/endosome tethering (CORVET) complex that tethers transport vesicles to the late endosome/multivesicular body (MVB). These results suggest that, of all the intracellular trafficking pathways, requirement of VLCFAs is especially high in the endosomal pathways.http://www.sciencedirect.com/science/article/pii/S0022227520419479membrane trafficmultivesicular bodyRabGTPaseyeast |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Keisuke Obara Ryo Kojima Akio Kihara |
spellingShingle |
Keisuke Obara Ryo Kojima Akio Kihara Effects on vesicular transport pathways at the late endosome in cells with limited very long-chain fatty acids[S] Journal of Lipid Research membrane traffic multivesicular body Rab GTPase yeast |
author_facet |
Keisuke Obara Ryo Kojima Akio Kihara |
author_sort |
Keisuke Obara |
title |
Effects on vesicular transport pathways at the late endosome in cells with limited very long-chain fatty acids[S] |
title_short |
Effects on vesicular transport pathways at the late endosome in cells with limited very long-chain fatty acids[S] |
title_full |
Effects on vesicular transport pathways at the late endosome in cells with limited very long-chain fatty acids[S] |
title_fullStr |
Effects on vesicular transport pathways at the late endosome in cells with limited very long-chain fatty acids[S] |
title_full_unstemmed |
Effects on vesicular transport pathways at the late endosome in cells with limited very long-chain fatty acids[S] |
title_sort |
effects on vesicular transport pathways at the late endosome in cells with limited very long-chain fatty acids[s] |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2013-03-01 |
description |
Very long-chain fatty acids (VLCFAs), fatty acids with chain-length greater than 20 carbons, possess a wide range of biological functions. However, their roles at the molecular level remain largely unknown. In the present study, we screened for multicopy suppressors that rescued temperature-sensitive growth of VLCFA-limited yeast cells, and we identified the VPS21 gene, encoding a Rab GTPase, as such a suppressor. When the vps21Δ mutation was introduced into a deletion mutant of the SUR4 gene, which encodes a VLCFA elongase, a synthetic growth defect was observed. Endosome-mediated vesicular trafficking pathways, including endocytosis and the carboxypeptidase Y (CPY) pathway, were severely impaired in sur4Δ vps21Δ double mutants, while the AP-3 pathway that bypasses the endosome was unaffected. In addition, the sur4Δ mutant also exhibited a synthetic growth defect when combined with the deletion of VPS3, which encodes a subunit of the class C core vacuole/endosome tethering (CORVET) complex that tethers transport vesicles to the late endosome/multivesicular body (MVB). These results suggest that, of all the intracellular trafficking pathways, requirement of VLCFAs is especially high in the endosomal pathways. |
topic |
membrane traffic multivesicular body Rab GTPase yeast |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520419479 |
work_keys_str_mv |
AT keisukeobara effectsonvesiculartransportpathwaysatthelateendosomeincellswithlimitedverylongchainfattyacidss AT ryokojima effectsonvesiculartransportpathwaysatthelateendosomeincellswithlimitedverylongchainfattyacidss AT akiokihara effectsonvesiculartransportpathwaysatthelateendosomeincellswithlimitedverylongchainfattyacidss |
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1721504217870368768 |