Molecularly defined extraintestinal pathogenic Escherichia coli status predicts virulence in a murine sepsis model better than does virotype, individual virulence genes, or clonal subset among E. coli ST131 isolates
Background: Escherichia coli ST131, mainly its H30 clade, is the leading cause of extraintestinal E. coli infections but its correlates of virulence are undefined. Materials and methods: We tested in a murine sepsis model 84 ST131 isolates that differed by country of origin (Spain vs. USA), clonal s...
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doaj-a6121412f42142cf8f2a4f9e1c9088a32021-01-15T14:09:06ZengTaylor & Francis GroupVirulence2150-55942150-56082020-01-0111132733610.1080/21505594.2020.17477991747799Molecularly defined extraintestinal pathogenic Escherichia coli status predicts virulence in a murine sepsis model better than does virotype, individual virulence genes, or clonal subset among E. coli ST131 isolatesIrene Merino0Stephen B. Porter1Brian Johnston2Connie Clabots3Paul Thuras4Patricia Ruiz-Garbajosa5Rafael Cantón6James R. Johnson7Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS)Minneapolis Veterans Health Care SystemUniversity of MinnesotaMinneapolis Veterans Health Care SystemMinneapolis Veterans Health Care SystemHospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS)Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS)Minneapolis Veterans Health Care SystemBackground: Escherichia coli ST131, mainly its H30 clade, is the leading cause of extraintestinal E. coli infections but its correlates of virulence are undefined. Materials and methods: We tested in a murine sepsis model 84 ST131 isolates that differed by country of origin (Spain vs. USA), clonal subset, resistance markers, and virulence genes (VGs). Virulence outcomes, including illness severity score (ISS) and “killer” status (>80% mouse lethality), were compared statistically with clonal subset, individual and combined VGs, molecularly defined extraintestinal and uropathogenic E. coli (ExPEC, UPEC) status, and country of origin. Results: Virulence varied widely by strain. Univariable correlates of median ISS and percent “killer” (outcomes if variable present vs. absent) included pap (ISS, 4.4 vs. 3.8; “killer”, 71% vs. 46%), kpsMII (4.1 vs. 2.3; 59% vs. 25%), K2/K100 (4.4 vs. 3.2; 77% vs. 41%), ExPEC (4.2 vs. 2.2; 62% vs. 17%), Spanish origin (4.3 vs. 3.1; 65% vs. 36%), and H30R1 subset (2.5 vs. 4.1; 35% vs. 59%). With multivariable adjustment, ExPEC status was the only consistently significantly predictive variable. Conclusion: Within ST131 the strongest predictor of experimental virulence was molecularly defined ExPEC status. Clonal subsets seemed to behave differently in the murine sepsis model by country of origin.http://dx.doi.org/10.1080/21505594.2020.1747799e. colivirulenceexpecmouse sepsis model |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Irene Merino Stephen B. Porter Brian Johnston Connie Clabots Paul Thuras Patricia Ruiz-Garbajosa Rafael Cantón James R. Johnson |
spellingShingle |
Irene Merino Stephen B. Porter Brian Johnston Connie Clabots Paul Thuras Patricia Ruiz-Garbajosa Rafael Cantón James R. Johnson Molecularly defined extraintestinal pathogenic Escherichia coli status predicts virulence in a murine sepsis model better than does virotype, individual virulence genes, or clonal subset among E. coli ST131 isolates Virulence e. coli virulence expec mouse sepsis model |
author_facet |
Irene Merino Stephen B. Porter Brian Johnston Connie Clabots Paul Thuras Patricia Ruiz-Garbajosa Rafael Cantón James R. Johnson |
author_sort |
Irene Merino |
title |
Molecularly defined extraintestinal pathogenic Escherichia coli status predicts virulence in a murine sepsis model better than does virotype, individual virulence genes, or clonal subset among E. coli ST131 isolates |
title_short |
Molecularly defined extraintestinal pathogenic Escherichia coli status predicts virulence in a murine sepsis model better than does virotype, individual virulence genes, or clonal subset among E. coli ST131 isolates |
title_full |
Molecularly defined extraintestinal pathogenic Escherichia coli status predicts virulence in a murine sepsis model better than does virotype, individual virulence genes, or clonal subset among E. coli ST131 isolates |
title_fullStr |
Molecularly defined extraintestinal pathogenic Escherichia coli status predicts virulence in a murine sepsis model better than does virotype, individual virulence genes, or clonal subset among E. coli ST131 isolates |
title_full_unstemmed |
Molecularly defined extraintestinal pathogenic Escherichia coli status predicts virulence in a murine sepsis model better than does virotype, individual virulence genes, or clonal subset among E. coli ST131 isolates |
title_sort |
molecularly defined extraintestinal pathogenic escherichia coli status predicts virulence in a murine sepsis model better than does virotype, individual virulence genes, or clonal subset among e. coli st131 isolates |
publisher |
Taylor & Francis Group |
series |
Virulence |
issn |
2150-5594 2150-5608 |
publishDate |
2020-01-01 |
description |
Background: Escherichia coli ST131, mainly its H30 clade, is the leading cause of extraintestinal E. coli infections but its correlates of virulence are undefined. Materials and methods: We tested in a murine sepsis model 84 ST131 isolates that differed by country of origin (Spain vs. USA), clonal subset, resistance markers, and virulence genes (VGs). Virulence outcomes, including illness severity score (ISS) and “killer” status (>80% mouse lethality), were compared statistically with clonal subset, individual and combined VGs, molecularly defined extraintestinal and uropathogenic E. coli (ExPEC, UPEC) status, and country of origin. Results: Virulence varied widely by strain. Univariable correlates of median ISS and percent “killer” (outcomes if variable present vs. absent) included pap (ISS, 4.4 vs. 3.8; “killer”, 71% vs. 46%), kpsMII (4.1 vs. 2.3; 59% vs. 25%), K2/K100 (4.4 vs. 3.2; 77% vs. 41%), ExPEC (4.2 vs. 2.2; 62% vs. 17%), Spanish origin (4.3 vs. 3.1; 65% vs. 36%), and H30R1 subset (2.5 vs. 4.1; 35% vs. 59%). With multivariable adjustment, ExPEC status was the only consistently significantly predictive variable. Conclusion: Within ST131 the strongest predictor of experimental virulence was molecularly defined ExPEC status. Clonal subsets seemed to behave differently in the murine sepsis model by country of origin. |
topic |
e. coli virulence expec mouse sepsis model |
url |
http://dx.doi.org/10.1080/21505594.2020.1747799 |
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