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mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} </style> <![endif]--> <p class="MsoNormal" style="margin-bottom: 0.0001pt; text-align: justify; line-height: normal;"><span style="font-size: 10pt; font-family: ";Cambria";,";serif";;">Con el fin de establecer una metodología para la obtención de regenerantes de arveja a partir de diferentes tipos de explantes, se evaluó el efecto de diferentes concentraciones de bencilaminopurina (BAP), ácido 2,4-diclorofenoxiacético (2,4-D) y 2-isopenteniladenina (2-ip) sobre la obtención de regenerantes para uso en transformación genética, como parte del proyecto de introducción de resistencia a Fusarium oxysporumf. sp.pisi, causante de la enfermedad marchitez vascular. Se evaluaron varios tipos de explantes, cotiledones en posición abaxial y adaxial, hipocótilos, epicótilos y embriones cigóticos obtenidos de semillas secas y semillas jóvenes. Estos explantes se evaluaron en interacción con reguladores de crecimiento bajo dos condiciones de luz: la primera, de fotoperíodo con 16 h de luz y 8 h de oscuridad y la segunda, de oscuridad total. De esta forma, se probaron tres reguladores de crecimiento (2,4-D, BAP y 2-ip) en tres combinaciones y diferentes concentraciones. Se obtuvieron regenerantes a partir de embriones cigóticos bajo las dos condiciones de luz, especialmente en la condición de fotoperíodo y con la aplicación de BAP en concentraciones de 1,0 y 2,5 mg· L-1. </span></p>
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