Establecimiento de una metodología para la inducción de regenerantes de arveja (Pisum sativum L.) variedad ‘Santa Isabel’

• Main Authors: Sánchez Edgar Alexander, Mosquera Teresa
• Format: Article
• Language: English
• Published: Centro Editorial of Facultad de Ciencias Agrarias, Universidad Nacional de Colombia 2006-06-01
• Series: Agronomía Colombiana
• Online Access: http://www.revistas.unal.edu.co/index.php/agrocol/article/view/20004

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 </style> <![endif]--> <p class="MsoNormal" style="margin-bottom: 0.0001pt; text-align: justify; line-height: normal;"><span style="font-size: 10pt; font-family: ";Cambria";,";serif";;">Con el fin de establecer una metodología para la obtención de regenerantes de arveja a partir de diferentes tipos de explantes, se evaluó el efecto de diferentes concentraciones de bencilaminopurina (BAP), ácido 2,4-diclorofenoxiacético (2,4-D) y 2-isopenteniladenina (2-ip) sobre la obtención de regenerantes para uso en transformación genética, como parte del proyecto de introducción de resistencia a Fusarium oxysporumf. sp.pisi, causante de la enfermedad marchitez vascular. Se evaluaron varios tipos de explantes, cotiledones en posición abaxial y adaxial, hipocótilos, epicótilos y embriones cigóticos obtenidos de semillas secas y semillas jóvenes. Estos explantes se evaluaron en interacción con reguladores de crecimiento bajo dos condiciones de luz: la primera, de fotoperíodo con 16 h de luz y 8 h de oscuridad y la segunda, de oscuridad total. De esta forma, se probaron tres reguladores de crecimiento (2,4-D, BAP y 2-ip) en tres combinaciones y diferentes concentraciones. Se obtuvieron regenerantes a partir de embriones cigóticos bajo las dos condiciones de luz, especialmente en la condición de fotoperíodo y con la aplicación de BAP en concentraciones de 1,0 y 2,5 mg· L-1. </span></p>