Impact of Formate Supplementation on Body Weight and Plasma Amino Acids
Current nutritional recommendations are focused on energy, fat, carbohydrate, protein and vitamins. Less attention has been paid to the nutritional demand of one-carbon units for nucleotide and methionine synthesis. Here, we investigated the impact of sodium formate supplementation as a nutritional...
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doaj-a633024cab7a40c69edb44820ab47d1d2020-11-25T03:05:50ZengMDPI AGNutrients2072-66432020-07-01122181218110.3390/nu12082181Impact of Formate Supplementation on Body Weight and Plasma Amino AcidsSandeep Dhayade0Matthias Pietzke1Robert Wiesheu2Jacqueline Tait-Mulder3Dimitris Athineos4David Sumpton5Seth Coffelt6Karen Blyth7Alexei Vazquez8Cancer Research UK Beatson Institute, Glasgow G61 1BD, UKCancer Research UK Beatson Institute, Glasgow G61 1BD, UKCancer Research UK Beatson Institute, Glasgow G61 1BD, UKCancer Research UK Beatson Institute, Glasgow G61 1BD, UKCancer Research UK Beatson Institute, Glasgow G61 1BD, UKCancer Research UK Beatson Institute, Glasgow G61 1BD, UKCancer Research UK Beatson Institute, Glasgow G61 1BD, UKCancer Research UK Beatson Institute, Glasgow G61 1BD, UKCancer Research UK Beatson Institute, Glasgow G61 1BD, UKCurrent nutritional recommendations are focused on energy, fat, carbohydrate, protein and vitamins. Less attention has been paid to the nutritional demand of one-carbon units for nucleotide and methionine synthesis. Here, we investigated the impact of sodium formate supplementation as a nutritional intervention to increase the dietary intake of one-carbon units. A cohort of six female and six male mice received 125 mM of sodium formate in the drinking water for three months. A control group of another six female and six male mice was also followed up for the same period of time. Tail vein blood samples were collected once a month and profiled with a haematology analyser. At the end of the study, blood and tissues were collected for metabolomics analysis and immune cell profiling. Formate supplementation had no significant physiological effect on male mice, except for a small decrease in body weight. Formate supplementation had no significant effect on the immune cell counts during the intervention or at the end of the study in either gender. In female mice, however, the body weight and spleen wet weight were significantly increased by formate supplementation, while the blood plasma levels of amino acids were decreased. Formate supplementation also increased the frequency of bifidobacteria, a probiotic bacterium, in the stools of female mice. We conclude that formate supplementation induces physiological changes in a gender-specific manner.https://www.mdpi.com/2072-6643/12/8/2181formateimmune systembody weightmicrobiomemetabolomics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sandeep Dhayade Matthias Pietzke Robert Wiesheu Jacqueline Tait-Mulder Dimitris Athineos David Sumpton Seth Coffelt Karen Blyth Alexei Vazquez |
spellingShingle |
Sandeep Dhayade Matthias Pietzke Robert Wiesheu Jacqueline Tait-Mulder Dimitris Athineos David Sumpton Seth Coffelt Karen Blyth Alexei Vazquez Impact of Formate Supplementation on Body Weight and Plasma Amino Acids Nutrients formate immune system body weight microbiome metabolomics |
author_facet |
Sandeep Dhayade Matthias Pietzke Robert Wiesheu Jacqueline Tait-Mulder Dimitris Athineos David Sumpton Seth Coffelt Karen Blyth Alexei Vazquez |
author_sort |
Sandeep Dhayade |
title |
Impact of Formate Supplementation on Body Weight and Plasma Amino Acids |
title_short |
Impact of Formate Supplementation on Body Weight and Plasma Amino Acids |
title_full |
Impact of Formate Supplementation on Body Weight and Plasma Amino Acids |
title_fullStr |
Impact of Formate Supplementation on Body Weight and Plasma Amino Acids |
title_full_unstemmed |
Impact of Formate Supplementation on Body Weight and Plasma Amino Acids |
title_sort |
impact of formate supplementation on body weight and plasma amino acids |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2020-07-01 |
description |
Current nutritional recommendations are focused on energy, fat, carbohydrate, protein and vitamins. Less attention has been paid to the nutritional demand of one-carbon units for nucleotide and methionine synthesis. Here, we investigated the impact of sodium formate supplementation as a nutritional intervention to increase the dietary intake of one-carbon units. A cohort of six female and six male mice received 125 mM of sodium formate in the drinking water for three months. A control group of another six female and six male mice was also followed up for the same period of time. Tail vein blood samples were collected once a month and profiled with a haematology analyser. At the end of the study, blood and tissues were collected for metabolomics analysis and immune cell profiling. Formate supplementation had no significant physiological effect on male mice, except for a small decrease in body weight. Formate supplementation had no significant effect on the immune cell counts during the intervention or at the end of the study in either gender. In female mice, however, the body weight and spleen wet weight were significantly increased by formate supplementation, while the blood plasma levels of amino acids were decreased. Formate supplementation also increased the frequency of bifidobacteria, a probiotic bacterium, in the stools of female mice. We conclude that formate supplementation induces physiological changes in a gender-specific manner. |
topic |
formate immune system body weight microbiome metabolomics |
url |
https://www.mdpi.com/2072-6643/12/8/2181 |
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