A new synthetic biology approach allows transfer of an entire metabolic pathway from a medicinal plant to a biomass crop
Artemisinin-based therapies are the only effective treatment for malaria, the most devastating disease in human history. To meet the growing demand for artemisinin and make it accessible to the poorest, an inexpensive and rapidly scalable production platform is urgently needed. Here we have develope...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
eLife Sciences Publications Ltd
2016-06-01
|
Series: | eLife |
Subjects: | |
Online Access: | https://elifesciences.org/articles/13664 |
id |
doaj-a642bb7a3c2048a3a0df06ece82cc6fe |
---|---|
record_format |
Article |
spelling |
doaj-a642bb7a3c2048a3a0df06ece82cc6fe2021-05-05T00:26:31ZengeLife Sciences Publications LtdeLife2050-084X2016-06-01510.7554/eLife.13664A new synthetic biology approach allows transfer of an entire metabolic pathway from a medicinal plant to a biomass cropPaulina Fuentes0Fei Zhou1Alexander Erban2Daniel Karcher3Joachim Kopka4Ralph Bock5https://orcid.org/0000-0001-7502-6940Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam-Golm, GermanyMax-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam-Golm, GermanyMax-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam-Golm, GermanyMax-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam-Golm, GermanyMax-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam-Golm, GermanyMax-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam-Golm, GermanyArtemisinin-based therapies are the only effective treatment for malaria, the most devastating disease in human history. To meet the growing demand for artemisinin and make it accessible to the poorest, an inexpensive and rapidly scalable production platform is urgently needed. Here we have developed a new synthetic biology approach, combinatorial supertransformation of transplastomic recipient lines (COSTREL), and applied it to introduce the complete pathway for artemisinic acid, the precursor of artemisinin, into the high-biomass crop tobacco. We first introduced the core pathway of artemisinic acid biosynthesis into the chloroplast genome. The transplastomic plants were then combinatorially supertransformed with cassettes for all additional enzymes known to affect flux through the artemisinin pathway. By screening large populations of COSTREL lines, we isolated plants that produce more than 120 milligram artemisinic acid per kilogram biomass. Our work provides an efficient strategy for engineering complex biochemical pathways into plants and optimizing the metabolic output.https://elifesciences.org/articles/13664synthetic biologymetabolic engineeringplastid transformationcombinatorial transformationNicotiana tabacumartemisinin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Paulina Fuentes Fei Zhou Alexander Erban Daniel Karcher Joachim Kopka Ralph Bock |
spellingShingle |
Paulina Fuentes Fei Zhou Alexander Erban Daniel Karcher Joachim Kopka Ralph Bock A new synthetic biology approach allows transfer of an entire metabolic pathway from a medicinal plant to a biomass crop eLife synthetic biology metabolic engineering plastid transformation combinatorial transformation Nicotiana tabacum artemisinin |
author_facet |
Paulina Fuentes Fei Zhou Alexander Erban Daniel Karcher Joachim Kopka Ralph Bock |
author_sort |
Paulina Fuentes |
title |
A new synthetic biology approach allows transfer of an entire metabolic pathway from a medicinal plant to a biomass crop |
title_short |
A new synthetic biology approach allows transfer of an entire metabolic pathway from a medicinal plant to a biomass crop |
title_full |
A new synthetic biology approach allows transfer of an entire metabolic pathway from a medicinal plant to a biomass crop |
title_fullStr |
A new synthetic biology approach allows transfer of an entire metabolic pathway from a medicinal plant to a biomass crop |
title_full_unstemmed |
A new synthetic biology approach allows transfer of an entire metabolic pathway from a medicinal plant to a biomass crop |
title_sort |
new synthetic biology approach allows transfer of an entire metabolic pathway from a medicinal plant to a biomass crop |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2016-06-01 |
description |
Artemisinin-based therapies are the only effective treatment for malaria, the most devastating disease in human history. To meet the growing demand for artemisinin and make it accessible to the poorest, an inexpensive and rapidly scalable production platform is urgently needed. Here we have developed a new synthetic biology approach, combinatorial supertransformation of transplastomic recipient lines (COSTREL), and applied it to introduce the complete pathway for artemisinic acid, the precursor of artemisinin, into the high-biomass crop tobacco. We first introduced the core pathway of artemisinic acid biosynthesis into the chloroplast genome. The transplastomic plants were then combinatorially supertransformed with cassettes for all additional enzymes known to affect flux through the artemisinin pathway. By screening large populations of COSTREL lines, we isolated plants that produce more than 120 milligram artemisinic acid per kilogram biomass. Our work provides an efficient strategy for engineering complex biochemical pathways into plants and optimizing the metabolic output. |
topic |
synthetic biology metabolic engineering plastid transformation combinatorial transformation Nicotiana tabacum artemisinin |
url |
https://elifesciences.org/articles/13664 |
work_keys_str_mv |
AT paulinafuentes anewsyntheticbiologyapproachallowstransferofanentiremetabolicpathwayfromamedicinalplanttoabiomasscrop AT feizhou anewsyntheticbiologyapproachallowstransferofanentiremetabolicpathwayfromamedicinalplanttoabiomasscrop AT alexandererban anewsyntheticbiologyapproachallowstransferofanentiremetabolicpathwayfromamedicinalplanttoabiomasscrop AT danielkarcher anewsyntheticbiologyapproachallowstransferofanentiremetabolicpathwayfromamedicinalplanttoabiomasscrop AT joachimkopka anewsyntheticbiologyapproachallowstransferofanentiremetabolicpathwayfromamedicinalplanttoabiomasscrop AT ralphbock anewsyntheticbiologyapproachallowstransferofanentiremetabolicpathwayfromamedicinalplanttoabiomasscrop AT paulinafuentes newsyntheticbiologyapproachallowstransferofanentiremetabolicpathwayfromamedicinalplanttoabiomasscrop AT feizhou newsyntheticbiologyapproachallowstransferofanentiremetabolicpathwayfromamedicinalplanttoabiomasscrop AT alexandererban newsyntheticbiologyapproachallowstransferofanentiremetabolicpathwayfromamedicinalplanttoabiomasscrop AT danielkarcher newsyntheticbiologyapproachallowstransferofanentiremetabolicpathwayfromamedicinalplanttoabiomasscrop AT joachimkopka newsyntheticbiologyapproachallowstransferofanentiremetabolicpathwayfromamedicinalplanttoabiomasscrop AT ralphbock newsyntheticbiologyapproachallowstransferofanentiremetabolicpathwayfromamedicinalplanttoabiomasscrop |
_version_ |
1721476290423291904 |