The Potential Utility of Curcumin in the Treatment of HER-2-Overexpressed Breast Cancer: An In Vitro and In Vivo Comparison Study with Herceptin
HER-2 is an important oncoprotein overexpressed in about 15–25% of breast cancers. We hypothesized that the ability of curcumin to downregulate HER-2 oncoprotein and inhibit the signal transduction pathway of PI3K/Akt, MAPK, and NF-κB activation may be important in the treatment of HER-2-overexpress...
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doaj-a64fd76b68de4abca5c121c7c704f1c62020-11-25T01:04:23ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882012-01-01201210.1155/2012/486568486568The Potential Utility of Curcumin in the Treatment of HER-2-Overexpressed Breast Cancer: An In Vitro and In Vivo Comparison Study with HerceptinHung-Wen Lai0Su-Yu Chien1Shou-Jen Kuo2Ling-Ming Tseng3Hui-Yi Lin4Chin-Wen Chi5Dar-Ren Chen6Comprehensive Breast Cancer Center, Changhua Christian Hospital, Changhua 50006, TaiwanDepartment of Pharmacology, Changhua Christian Hospital, Changhua 50006, TaiwanComprehensive Breast Cancer Center, Changhua Christian Hospital, Changhua 50006, TaiwanDivision of General Surgery, Department of Surgery, Taipei Veterans General Hospital, Taipei 11217, TaiwanDepartment of Pharmacology, School of Pharmacology, China Medical University, Taichung 40402, TaiwanDepartment and Institute of Pharmacology, School of Medicine, National Yang-Ming University, Taipei 11221, TaiwanComprehensive Breast Cancer Center, Changhua Christian Hospital, Changhua 50006, TaiwanHER-2 is an important oncoprotein overexpressed in about 15–25% of breast cancers. We hypothesized that the ability of curcumin to downregulate HER-2 oncoprotein and inhibit the signal transduction pathway of PI3K/Akt, MAPK, and NF-κB activation may be important in the treatment of HER-2-overexpressed breast cancer. To examine the effect of curcumin on breast cancer cells, MCF-7, MDA-MB-231, MCF-10A, BT-474, and SK-BR-3-hr (a herceptin resistant strain from SK-BR-3) cells were used for in vitro analysis. The in vivo effect of curcumin on HER-2-overexpressed breast cancer was investigated with the HER-2-overexpressed BT-474 xenograft model. Cell growth, cell cycle change, the antimobility effect, signal transduction, and xenograft volume analysis between groups treated with herceptin and/or curcumin were tested. Curcumin decreased the cell growth of various breast cancer cell lines (MCF-7, MDA-MB-231, MCF-10A, BT-474, and SK-BR-3-hr). In Western blot analysis, the phosphorylation of Akt, MAPK, and expression of NF-κB were reduced in BT-474 cells, but not in SK-BR-3-hr cells, after treatment with herceptin. When treated with curcumin, the HER-2 oncoprotein, phosphorylation of Akt, MAPK and expression of NF-κB were decreased in both BT-474 and SK-BR-3-hr cells. In the BT-474 xenograft model, though not as much as herceptin, curcumin did effectively decrease the tumor size. The combination of curcumin with herceptin was not better than herceptin alone; however, the combination of taxol and curcumin had an antitumor effect comparable with taxol and herceptin. The results suggested that curcumin has potential as a treatment for HER-2-overexpressed breast cancer.http://dx.doi.org/10.1155/2012/486568 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hung-Wen Lai Su-Yu Chien Shou-Jen Kuo Ling-Ming Tseng Hui-Yi Lin Chin-Wen Chi Dar-Ren Chen |
spellingShingle |
Hung-Wen Lai Su-Yu Chien Shou-Jen Kuo Ling-Ming Tseng Hui-Yi Lin Chin-Wen Chi Dar-Ren Chen The Potential Utility of Curcumin in the Treatment of HER-2-Overexpressed Breast Cancer: An In Vitro and In Vivo Comparison Study with Herceptin Evidence-Based Complementary and Alternative Medicine |
author_facet |
Hung-Wen Lai Su-Yu Chien Shou-Jen Kuo Ling-Ming Tseng Hui-Yi Lin Chin-Wen Chi Dar-Ren Chen |
author_sort |
Hung-Wen Lai |
title |
The Potential Utility of Curcumin in the Treatment of HER-2-Overexpressed Breast Cancer: An In Vitro and In Vivo Comparison Study with Herceptin |
title_short |
The Potential Utility of Curcumin in the Treatment of HER-2-Overexpressed Breast Cancer: An In Vitro and In Vivo Comparison Study with Herceptin |
title_full |
The Potential Utility of Curcumin in the Treatment of HER-2-Overexpressed Breast Cancer: An In Vitro and In Vivo Comparison Study with Herceptin |
title_fullStr |
The Potential Utility of Curcumin in the Treatment of HER-2-Overexpressed Breast Cancer: An In Vitro and In Vivo Comparison Study with Herceptin |
title_full_unstemmed |
The Potential Utility of Curcumin in the Treatment of HER-2-Overexpressed Breast Cancer: An In Vitro and In Vivo Comparison Study with Herceptin |
title_sort |
potential utility of curcumin in the treatment of her-2-overexpressed breast cancer: an in vitro and in vivo comparison study with herceptin |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2012-01-01 |
description |
HER-2 is an important oncoprotein overexpressed in about 15–25% of breast cancers. We hypothesized that the ability of curcumin to downregulate HER-2 oncoprotein and inhibit the signal transduction pathway of PI3K/Akt, MAPK, and NF-κB activation may be important in the treatment of HER-2-overexpressed breast cancer. To examine the effect of curcumin on breast cancer cells, MCF-7, MDA-MB-231, MCF-10A, BT-474, and SK-BR-3-hr (a herceptin resistant strain from SK-BR-3) cells were used for in vitro analysis. The in vivo effect of curcumin on HER-2-overexpressed breast cancer was investigated with the HER-2-overexpressed BT-474 xenograft model. Cell growth, cell cycle change, the antimobility effect, signal transduction, and xenograft volume analysis between groups treated with herceptin and/or curcumin were tested. Curcumin decreased the cell growth of various breast cancer cell lines (MCF-7, MDA-MB-231, MCF-10A, BT-474, and SK-BR-3-hr). In Western blot analysis, the phosphorylation of Akt, MAPK, and expression of NF-κB were reduced in BT-474 cells, but not in SK-BR-3-hr cells, after treatment with herceptin. When treated with curcumin, the HER-2 oncoprotein, phosphorylation of Akt, MAPK and expression of NF-κB were decreased in both BT-474 and SK-BR-3-hr cells. In the BT-474 xenograft model, though not as much as herceptin, curcumin did effectively decrease the tumor size. The combination of curcumin with herceptin was not better than herceptin alone; however, the combination of taxol and curcumin had an antitumor effect comparable with taxol and herceptin. The results suggested that curcumin has potential as a treatment for HER-2-overexpressed breast cancer. |
url |
http://dx.doi.org/10.1155/2012/486568 |
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