Expression and interaction of CDC25A and IL-6 in hepatocellular carcinoma HepG2 cells
Objective: This study was to further investigate the effect of CDC25A gene on proliferation, invasion and metastasis of hepatocellular carcinoma HepG2 cells, and to explore whether CDC25A and IL6 interact in liver cancer. Methods: HepG2 cells were transfected with lentiviral CDC25A-shRNA to speci...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Editorial Board of Journal of Hainan Medical University
2019-07-01
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Series: | Journal of Hainan Medical University |
Subjects: | |
Online Access: | http://www.hnykdxxb.com/PDF/201913/01.pdf |
Summary: | Objective: This study was to further investigate the effect of CDC25A gene on proliferation,
invasion and metastasis of hepatocellular carcinoma HepG2 cells, and to explore whether
CDC25A and IL6 interact in liver cancer. Methods: HepG2 cells were transfected
with lentiviral CDC25A-shRNA to specifically block the expression of CDC25A in the
experimental group (KD group), transfected with lentiviral-negative shRNA as a negative
control group (NC group), and the conventionally cultured HepG2 cells were blank control
group (Control group). Real-time quantitative PCR and Western blot were used to detect the
mRNA and protein expression levels of CDC25A and IL6. Results: The expression level of
IL6 mRNA in the experimental group was 0.4650.026, which was significantly lower than
that of the negative control group of 1.0940.179 and the blank control group of 1.0020.311.
The difference was statistically significant(P<0.05). The protein expression level of IL6 was
0.4990.061, which was significantly lower than the negative control group of 1.3110.034 and
the blank control group of 1.2530.035. The difference was statistically significant (P<0.05).
Conclusions: The CDC25A gene in hepatoma HepG2 cells was silenced, while the expression
of IL6 was down-regulated. CDC25A may play a role in the development and invasion of liver
cancer by regulating the expression of IL6. |
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ISSN: | 1007-1237 1007-1237 |