Cellular delivery and photochemical release of a caged inositol-pyrophosphate induces PH-domain translocation in cellulo
Photocaged inositol-pyrophosphates offer a tool to study cellular signalling, but their challenging synthesis has precluded any biological studies so far. Here, the authors report the synthesis and cellular delivery of a photocaged analogue, and show that it mediates protein translocation in cellulo...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2016-02-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms10622 |
| id |
doaj-a6bac542d07b431a964d07a618a8a4d6 |
|---|---|
| record_format |
Article |
| spelling |
doaj-a6bac542d07b431a964d07a618a8a4d62021-05-11T10:48:27ZengNature Publishing GroupNature Communications2041-17232016-02-01711810.1038/ncomms10622Cellular delivery and photochemical release of a caged inositol-pyrophosphate induces PH-domain translocation in celluloIgor Pavlovic0Divyeshsinh T. Thakor1Jessica R. Vargas2Colin J. McKinlay3Sebastian Hauke4Philipp Anstaett5Rafael C. Camuña6Laurent Bigler7Gilles Gasser8Carsten Schultz9Paul A. Wender10Henning J. Jessen11Department of Chemistry, University of ZurichDepartment of Chemistry, University of ZurichDepartments of Chemistry and Chemical and Systems Biology, Stanford UniversityDepartments of Chemistry and Chemical and Systems Biology, Stanford UniversityEuropean Molecular Biology Laboratory (EMBL), Cell Biology & Biophysics UnitDepartment of Chemistry, University of ZurichDepartamento de Química Orgánica, Facultad de Ciencias, Universidad de MálagaDepartment of Chemistry, University of ZurichDepartment of Chemistry, University of ZurichEuropean Molecular Biology Laboratory (EMBL), Cell Biology & Biophysics UnitDepartments of Chemistry and Chemical and Systems Biology, Stanford UniversityDepartment of Chemistry and Pharmacy, Albert-Ludwigs University FreiburgPhotocaged inositol-pyrophosphates offer a tool to study cellular signalling, but their challenging synthesis has precluded any biological studies so far. Here, the authors report the synthesis and cellular delivery of a photocaged analogue, and show that it mediates protein translocation in cellulo.https://doi.org/10.1038/ncomms10622 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Igor Pavlovic Divyeshsinh T. Thakor Jessica R. Vargas Colin J. McKinlay Sebastian Hauke Philipp Anstaett Rafael C. Camuña Laurent Bigler Gilles Gasser Carsten Schultz Paul A. Wender Henning J. Jessen |
| spellingShingle |
Igor Pavlovic Divyeshsinh T. Thakor Jessica R. Vargas Colin J. McKinlay Sebastian Hauke Philipp Anstaett Rafael C. Camuña Laurent Bigler Gilles Gasser Carsten Schultz Paul A. Wender Henning J. Jessen Cellular delivery and photochemical release of a caged inositol-pyrophosphate induces PH-domain translocation in cellulo Nature Communications |
| author_facet |
Igor Pavlovic Divyeshsinh T. Thakor Jessica R. Vargas Colin J. McKinlay Sebastian Hauke Philipp Anstaett Rafael C. Camuña Laurent Bigler Gilles Gasser Carsten Schultz Paul A. Wender Henning J. Jessen |
| author_sort |
Igor Pavlovic |
| title |
Cellular delivery and photochemical release of a caged inositol-pyrophosphate induces PH-domain translocation in cellulo |
| title_short |
Cellular delivery and photochemical release of a caged inositol-pyrophosphate induces PH-domain translocation in cellulo |
| title_full |
Cellular delivery and photochemical release of a caged inositol-pyrophosphate induces PH-domain translocation in cellulo |
| title_fullStr |
Cellular delivery and photochemical release of a caged inositol-pyrophosphate induces PH-domain translocation in cellulo |
| title_full_unstemmed |
Cellular delivery and photochemical release of a caged inositol-pyrophosphate induces PH-domain translocation in cellulo |
| title_sort |
cellular delivery and photochemical release of a caged inositol-pyrophosphate induces ph-domain translocation in cellulo |
| publisher |
Nature Publishing Group |
| series |
Nature Communications |
| issn |
2041-1723 |
| publishDate |
2016-02-01 |
| description |
Photocaged inositol-pyrophosphates offer a tool to study cellular signalling, but their challenging synthesis has precluded any biological studies so far. Here, the authors report the synthesis and cellular delivery of a photocaged analogue, and show that it mediates protein translocation in cellulo. |
| url |
https://doi.org/10.1038/ncomms10622 |
| work_keys_str_mv |
AT igorpavlovic cellulardeliveryandphotochemicalreleaseofacagedinositolpyrophosphateinducesphdomaintranslocationincellulo AT divyeshsinhtthakor cellulardeliveryandphotochemicalreleaseofacagedinositolpyrophosphateinducesphdomaintranslocationincellulo AT jessicarvargas cellulardeliveryandphotochemicalreleaseofacagedinositolpyrophosphateinducesphdomaintranslocationincellulo AT colinjmckinlay cellulardeliveryandphotochemicalreleaseofacagedinositolpyrophosphateinducesphdomaintranslocationincellulo AT sebastianhauke cellulardeliveryandphotochemicalreleaseofacagedinositolpyrophosphateinducesphdomaintranslocationincellulo AT philippanstaett cellulardeliveryandphotochemicalreleaseofacagedinositolpyrophosphateinducesphdomaintranslocationincellulo AT rafaelccamuna cellulardeliveryandphotochemicalreleaseofacagedinositolpyrophosphateinducesphdomaintranslocationincellulo AT laurentbigler cellulardeliveryandphotochemicalreleaseofacagedinositolpyrophosphateinducesphdomaintranslocationincellulo AT gillesgasser cellulardeliveryandphotochemicalreleaseofacagedinositolpyrophosphateinducesphdomaintranslocationincellulo AT carstenschultz cellulardeliveryandphotochemicalreleaseofacagedinositolpyrophosphateinducesphdomaintranslocationincellulo AT paulawender cellulardeliveryandphotochemicalreleaseofacagedinositolpyrophosphateinducesphdomaintranslocationincellulo AT henningjjessen cellulardeliveryandphotochemicalreleaseofacagedinositolpyrophosphateinducesphdomaintranslocationincellulo |
| _version_ |
1721447533201326080 |