Isolation, Identification, and Biotransformation of Teadenol A from Solid State Fermentation of Pu-erh Tea and In Vitro Antioxidant Activity
Post-fermented Pu-erh tea (PFPT) has several health benefits, however, little is known about the bioactive compounds. In this study, a PFPT compound was isolated by column chromatography and identified as Teadenol A by spectroscopic data analyses, including mass spectrometry and 1D and 2D NMR spectr...
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2016-05-01
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| Series: | Applied Sciences |
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| Online Access: | http://www.mdpi.com/2076-3417/6/6/161 |
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doaj-a6ec31a3ad804a21874d15a7d86097b32020-11-24T21:07:56ZengMDPI AGApplied Sciences2076-34172016-05-016616110.3390/app6060161app6060161Isolation, Identification, and Biotransformation of Teadenol A from Solid State Fermentation of Pu-erh Tea and In Vitro Antioxidant ActivityXiao-qin Su0Gao-ju Zhang1Yan Ma2Mao Chen3Sheng-hu Chen4Shuang-mei Duan5Jin-qiong Wan6Fumio Hashimoto7Hai-peng Lv8Jia-hua Li9Zhi Lin10Ming Zhao11Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, ChinaYunnan Research Center on Good Agricultural Practice for Dominant Chinese Medicinal Materials, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaCollege of Longrun Pu-erh Tea, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaCollege of Longrun Pu-erh Tea, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaCollege of Longrun Pu-erh Tea, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaCollege of Longrun Pu-erh Tea, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaCollege of Longrun Pu-erh Tea, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaDepartment of Horticultural Science, Faculty of Agriculture, Kagoshima University, Kagoshima 890-0065, JapanKey Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, ChinaCollege of Longrun Pu-erh Tea, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaKey Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, ChinaKey Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, ChinaPost-fermented Pu-erh tea (PFPT) has several health benefits, however, little is known about the bioactive compounds. In this study, a PFPT compound was isolated by column chromatography and identified as Teadenol A by spectroscopic data analyses, including mass spectrometry and 1D and 2D NMR spectroscopy. Teadenol A in tea leaves was biotransformed by Aspergillus niger and A. tamari at 28 °C for 14 d at concentrations ranging from 9.85 ± 1.17 to 12.93 ± 0.38 mg/g. Additionally, the compound was detected in 22 commercial PFPTs at concentrations ranging from 0.17 ± 0.1 to 8.15 ± 0.1 mg/g. Teadenol A promoted the secretion of adiponectin and inhibited the expression of protein tyrosine phosphatase-1B. Antioxidant assays (e.g., 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, total antioxidant capacity (T-AOC), hydrogen donating ability, and superoxide anion radical scavenging capacity) revealed that Teadenol A has antioxidant properties. Therefore, Teadenol A is an important bio-active component of PFPT.http://www.mdpi.com/2076-3417/6/6/161Pu-erh teaTeadenol Aantioxidant activityAspergillus nigersolid state fermentation |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Xiao-qin Su Gao-ju Zhang Yan Ma Mao Chen Sheng-hu Chen Shuang-mei Duan Jin-qiong Wan Fumio Hashimoto Hai-peng Lv Jia-hua Li Zhi Lin Ming Zhao |
| spellingShingle |
Xiao-qin Su Gao-ju Zhang Yan Ma Mao Chen Sheng-hu Chen Shuang-mei Duan Jin-qiong Wan Fumio Hashimoto Hai-peng Lv Jia-hua Li Zhi Lin Ming Zhao Isolation, Identification, and Biotransformation of Teadenol A from Solid State Fermentation of Pu-erh Tea and In Vitro Antioxidant Activity Applied Sciences Pu-erh tea Teadenol A antioxidant activity Aspergillus niger solid state fermentation |
| author_facet |
Xiao-qin Su Gao-ju Zhang Yan Ma Mao Chen Sheng-hu Chen Shuang-mei Duan Jin-qiong Wan Fumio Hashimoto Hai-peng Lv Jia-hua Li Zhi Lin Ming Zhao |
| author_sort |
Xiao-qin Su |
| title |
Isolation, Identification, and Biotransformation of Teadenol A from Solid State Fermentation of Pu-erh Tea and In Vitro Antioxidant Activity |
| title_short |
Isolation, Identification, and Biotransformation of Teadenol A from Solid State Fermentation of Pu-erh Tea and In Vitro Antioxidant Activity |
| title_full |
Isolation, Identification, and Biotransformation of Teadenol A from Solid State Fermentation of Pu-erh Tea and In Vitro Antioxidant Activity |
| title_fullStr |
Isolation, Identification, and Biotransformation of Teadenol A from Solid State Fermentation of Pu-erh Tea and In Vitro Antioxidant Activity |
| title_full_unstemmed |
Isolation, Identification, and Biotransformation of Teadenol A from Solid State Fermentation of Pu-erh Tea and In Vitro Antioxidant Activity |
| title_sort |
isolation, identification, and biotransformation of teadenol a from solid state fermentation of pu-erh tea and in vitro antioxidant activity |
| publisher |
MDPI AG |
| series |
Applied Sciences |
| issn |
2076-3417 |
| publishDate |
2016-05-01 |
| description |
Post-fermented Pu-erh tea (PFPT) has several health benefits, however, little is known about the bioactive compounds. In this study, a PFPT compound was isolated by column chromatography and identified as Teadenol A by spectroscopic data analyses, including mass spectrometry and 1D and 2D NMR spectroscopy. Teadenol A in tea leaves was biotransformed by Aspergillus niger and A. tamari at 28 °C for 14 d at concentrations ranging from 9.85 ± 1.17 to 12.93 ± 0.38 mg/g. Additionally, the compound was detected in 22 commercial PFPTs at concentrations ranging from 0.17 ± 0.1 to 8.15 ± 0.1 mg/g. Teadenol A promoted the secretion of adiponectin and inhibited the expression of protein tyrosine phosphatase-1B. Antioxidant assays (e.g., 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, total antioxidant capacity (T-AOC), hydrogen donating ability, and superoxide anion radical scavenging capacity) revealed that Teadenol A has antioxidant properties. Therefore, Teadenol A is an important bio-active component of PFPT. |
| topic |
Pu-erh tea Teadenol A antioxidant activity Aspergillus niger solid state fermentation |
| url |
http://www.mdpi.com/2076-3417/6/6/161 |
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