The Protective Role of Mitochondrial Ferritin on Erastin-Induced Ferroptosis
Ferroptosis, a newly identified form of regulated cell death, is characterized by overwhelming iron-dependent accumulation of lethal lipid reactive oxygen species (ROS). Preventing cellular iron overload by reducing iron uptake and increasing iron storage may contribute to inhibit ferroptosis. Mitoc...
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doaj-a70fbd2a74094dd2b3eb2dc2e91c7dd92020-11-24T23:52:11ZengFrontiers Media S.A.Frontiers in Aging Neuroscience1663-43652016-12-01810.3389/fnagi.2016.00308220990The Protective Role of Mitochondrial Ferritin on Erastin-Induced FerroptosisYue-Qi Wang0Shi-Yang Chang1Qiong Wu2Yu-jing Gou3Linpei Jia4Cui Yan-Mei5Peng Yu6Zhen-Hua Shi7Wen-Shuang Wu8Guofen Gao9Yan-Zhong Chang10Hebei Normal UniversityHebei Normal UniversityHebei Normal UniversityHebei Normal UniversityThe Second Hospital of Jilin UniversityHebei Normal UniversityHebei Normal UniversityHebei Normal UniversityThe 3rd Hospital of Hebei Medical UniversityHebei Normal UniversityHebei Normal UniversityFerroptosis, a newly identified form of regulated cell death, is characterized by overwhelming iron-dependent accumulation of lethal lipid reactive oxygen species (ROS). Preventing cellular iron overload by reducing iron uptake and increasing iron storage may contribute to inhibit ferroptosis. Mitochondrial ferritin (FtMt) is an iron-storage protein that is located in the mitochondria, which has a significant role in modulating cellular iron metabolism. Recent studies showed that FtMt played inhibitory effects on oxidative stress-dependent neuronal cell damage. However, the potential role of FtMt in the progress of ferroptosis in neuronal cells has not been studied. To explore this, we established ferroptosis models of cell and drosophila by erastin treatment. We found that overexpression of FtMt in neuroblastoma SH-SY5Y cells significantly inhibited erastin-induced ferroptosis, which very likely was achieved by regulation of iron homeostasis. Upon erastin treatment, significant increases of cellular labile iron pool (LIP) and cytosolic ROS were observed in wild-type SH-SY5Y cells, but not in the FtMt-overexpressed cells. Consistent with that, the alterations of iron-related proteins in FtMt-overexpressed cells were different from that of the control cells. We further investigated the role of FtMt in erastin-induced ferroptosis in transgenic drosophila. We found that the wild-type drosophilas fed an erastin-containing diet didn’t survive more than three weeks. In contrast, the FtMt overexpressing drosophilas fed the same diet were survival very well. These results indicated that FtMt played a protective role in erastin-induced ferroptosis.http://journal.frontiersin.org/Journal/10.3389/fnagi.2016.00308/fullIronLipROSMitochondrial ferritinferroptosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yue-Qi Wang Shi-Yang Chang Qiong Wu Yu-jing Gou Linpei Jia Cui Yan-Mei Peng Yu Zhen-Hua Shi Wen-Shuang Wu Guofen Gao Yan-Zhong Chang |
spellingShingle |
Yue-Qi Wang Shi-Yang Chang Qiong Wu Yu-jing Gou Linpei Jia Cui Yan-Mei Peng Yu Zhen-Hua Shi Wen-Shuang Wu Guofen Gao Yan-Zhong Chang The Protective Role of Mitochondrial Ferritin on Erastin-Induced Ferroptosis Frontiers in Aging Neuroscience Iron Lip ROS Mitochondrial ferritin ferroptosis |
author_facet |
Yue-Qi Wang Shi-Yang Chang Qiong Wu Yu-jing Gou Linpei Jia Cui Yan-Mei Peng Yu Zhen-Hua Shi Wen-Shuang Wu Guofen Gao Yan-Zhong Chang |
author_sort |
Yue-Qi Wang |
title |
The Protective Role of Mitochondrial Ferritin on Erastin-Induced Ferroptosis |
title_short |
The Protective Role of Mitochondrial Ferritin on Erastin-Induced Ferroptosis |
title_full |
The Protective Role of Mitochondrial Ferritin on Erastin-Induced Ferroptosis |
title_fullStr |
The Protective Role of Mitochondrial Ferritin on Erastin-Induced Ferroptosis |
title_full_unstemmed |
The Protective Role of Mitochondrial Ferritin on Erastin-Induced Ferroptosis |
title_sort |
protective role of mitochondrial ferritin on erastin-induced ferroptosis |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Aging Neuroscience |
issn |
1663-4365 |
publishDate |
2016-12-01 |
description |
Ferroptosis, a newly identified form of regulated cell death, is characterized by overwhelming iron-dependent accumulation of lethal lipid reactive oxygen species (ROS). Preventing cellular iron overload by reducing iron uptake and increasing iron storage may contribute to inhibit ferroptosis. Mitochondrial ferritin (FtMt) is an iron-storage protein that is located in the mitochondria, which has a significant role in modulating cellular iron metabolism. Recent studies showed that FtMt played inhibitory effects on oxidative stress-dependent neuronal cell damage. However, the potential role of FtMt in the progress of ferroptosis in neuronal cells has not been studied. To explore this, we established ferroptosis models of cell and drosophila by erastin treatment. We found that overexpression of FtMt in neuroblastoma SH-SY5Y cells significantly inhibited erastin-induced ferroptosis, which very likely was achieved by regulation of iron homeostasis. Upon erastin treatment, significant increases of cellular labile iron pool (LIP) and cytosolic ROS were observed in wild-type SH-SY5Y cells, but not in the FtMt-overexpressed cells. Consistent with that, the alterations of iron-related proteins in FtMt-overexpressed cells were different from that of the control cells. We further investigated the role of FtMt in erastin-induced ferroptosis in transgenic drosophila. We found that the wild-type drosophilas fed an erastin-containing diet didn’t survive more than three weeks. In contrast, the FtMt overexpressing drosophilas fed the same diet were survival very well. These results indicated that FtMt played a protective role in erastin-induced ferroptosis. |
topic |
Iron Lip ROS Mitochondrial ferritin ferroptosis |
url |
http://journal.frontiersin.org/Journal/10.3389/fnagi.2016.00308/full |
work_keys_str_mv |
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