Metagenomic Analyses of Microbial and Carbohydrate-Active Enzymes in the Rumen of Dairy Goats Fed Different Rumen Degradable Starch

• Main Authors: Jing Shen, Lixin Zheng, Xiaodong Chen, Xiaoying Han, Yangchun Cao, Junhu Yao
• Format: Article
• Language: English
• Published: Frontiers Media S.A. 2020-05-01
• Series: Frontiers in Microbiology
• Subjects: metagenomics; microbiome; CAZymes; rumen degradable starch; dairy goats
• Online Access: https://www.frontiersin.org/article/10.3389/fmicb.2020.01003/full

The objective of this study was to investigate the effects of different dietary rumen degradable starch (RDS) on the diversity of carbohydrate-active enzymes (CAZymes) and Kyoto Encyclopedia of Genes and Genomes Orthology functional categories to explore carbohydrate degradation in dairy goats. Eighteen dairy goats (second lactation, 45.8 ± 1.54 kg) were divided in three groups fed low RDS (LRDS), medium RDS (MRDS), and high RDS (HRDS) diets. The results showed that, HRDS treatment group significantly decreased the ruminal pH (P < 0.05), and increased the propionate proportion (P < 0.05), fumarate and succinate concentrations (P < 0.05), trended to increase lactate concentration (P = 0.50) compared with LRDS group. The relative abundance of acetogens, such as family Clostridiaceae and Ruminococcaceae, genera Clostridium and Blautia were higher in HRDS than LRDS feeding goats. The GH9 family (responsible for cellulose degradation) genes were lower in HRDS than MRDS diet samples, and mainly produced by Prevotellaceae, Ruminococcaceae, and Bacteroidaceae. Amylose (EC3.2.1.3) genes under HRDS treatment were more abundant than under LRDS treatment. However, the abundance of GH13_9 and CBM48 (responsible for starch degradation) were reduced in HRDS group indicating the decreased binding activity from catalytic modules to starch. This study revealed that HRDS-fed dairy goats had decreased CAZymes, which encode enzymes degrade cellulose and starch in the dairy goats.