Immobilization of periodate oxidized invertase by adsorption on sepiolite

Periodate oxidized invertase was immobilized by adsorption on sepiolite. The obtained immobilized enzyme was more resistant to washing out by concentrated salt solution, and had an eight times higher half-life at 60ºC than adsorbed native invertase. In packed bed reactor 50 % conversion of 500 g/dm3...

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Main Authors: RADIVOJE M. PRODANOVIC, MILOS B. SIMIC, ZORAN M. VUJCIC
Format: Article
Language:English
Published: Serbian Chemical Society 2003-11-01
Series:Journal of the Serbian Chemical Society
Subjects:
Online Access:http://www.shd.org.yu/HtDocs/SHD/Vol68/No11/V68-No11-05.pdf
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spelling doaj-a72e46411d9c4be2824452073f2627912020-11-24T22:25:28ZengSerbian Chemical Society Journal of the Serbian Chemical Society0352-51392003-11-016811819824Immobilization of periodate oxidized invertase by adsorption on sepioliteRADIVOJE M. PRODANOVICMILOS B. SIMICZORAN M. VUJCICPeriodate oxidized invertase was immobilized by adsorption on sepiolite. The obtained immobilized enzyme was more resistant to washing out by concentrated salt solution, and had an eight times higher half-life at 60ºC than adsorbed native invertase. In packed bed reactor 50 % conversion of 500 g/dm3 sucrose at 40ºC and a flow rate of 1 bv/h was achieved. The specific productivity of the immobilized invertase was 0.187 kg/dm3/h.http://www.shd.org.yu/HtDocs/SHD/Vol68/No11/V68-No11-05.pdfinvertaseperiodate oxidationsepioliteimmobilizationsucrose
collection DOAJ
language English
format Article
sources DOAJ
author RADIVOJE M. PRODANOVIC
MILOS B. SIMIC
ZORAN M. VUJCIC
spellingShingle RADIVOJE M. PRODANOVIC
MILOS B. SIMIC
ZORAN M. VUJCIC
Immobilization of periodate oxidized invertase by adsorption on sepiolite
Journal of the Serbian Chemical Society
invertase
periodate oxidation
sepiolite
immobilization
sucrose
author_facet RADIVOJE M. PRODANOVIC
MILOS B. SIMIC
ZORAN M. VUJCIC
author_sort RADIVOJE M. PRODANOVIC
title Immobilization of periodate oxidized invertase by adsorption on sepiolite
title_short Immobilization of periodate oxidized invertase by adsorption on sepiolite
title_full Immobilization of periodate oxidized invertase by adsorption on sepiolite
title_fullStr Immobilization of periodate oxidized invertase by adsorption on sepiolite
title_full_unstemmed Immobilization of periodate oxidized invertase by adsorption on sepiolite
title_sort immobilization of periodate oxidized invertase by adsorption on sepiolite
publisher Serbian Chemical Society
series Journal of the Serbian Chemical Society
issn 0352-5139
publishDate 2003-11-01
description Periodate oxidized invertase was immobilized by adsorption on sepiolite. The obtained immobilized enzyme was more resistant to washing out by concentrated salt solution, and had an eight times higher half-life at 60ºC than adsorbed native invertase. In packed bed reactor 50 % conversion of 500 g/dm3 sucrose at 40ºC and a flow rate of 1 bv/h was achieved. The specific productivity of the immobilized invertase was 0.187 kg/dm3/h.
topic invertase
periodate oxidation
sepiolite
immobilization
sucrose
url http://www.shd.org.yu/HtDocs/SHD/Vol68/No11/V68-No11-05.pdf
work_keys_str_mv AT radivojemprodanovic immobilizationofperiodateoxidizedinvertasebyadsorptiononsepiolite
AT milosbsimic immobilizationofperiodateoxidizedinvertasebyadsorptiononsepiolite
AT zoranmvujcic immobilizationofperiodateoxidizedinvertasebyadsorptiononsepiolite
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