Genetic characterization of a reptilian calicivirus (Cro1)
<p>Abstract</p> <p>Background</p> <p>Vesiviruses in the family <it>Caliciviridae</it> infect a broad range of animal hosts including mammals, birds, fish, amphibians and reptiles. The vesivirus Cro1 strains were isolated from diseased snakes in the San Diego...
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doaj-a72ed85ff3a04c488b598e713c4b5b5a2020-11-25T00:04:55ZengBMCVirology Journal1743-422X2012-11-019129710.1186/1743-422X-9-297Genetic characterization of a reptilian calicivirus (Cro1)Sandoval-Jaime CarlosParra Gabriel ISmith Alvin WGreen Kim YSosnovtsev Stanislav V<p>Abstract</p> <p>Background</p> <p>Vesiviruses in the family <it>Caliciviridae</it> infect a broad range of animal hosts including mammals, birds, fish, amphibians and reptiles. The vesivirus Cro1 strains were isolated from diseased snakes in the San Diego zoo in 1978 and reported as the first caliciviruses found in reptiles. The goal of this study was to characterize the Cro1 strain 780032I that was isolated in cell culture from a rock rattlesnake (<it>Crotalus lepidus)</it> in the original outbreak.</p> <p>Results</p> <p>We re-amplified the original virus stock in Vero cells, and determined its full-length genome sequence. The Cro1 genome is 8296 nucleotides (nt) in length and has a typical vesivirus organization, with three open reading frames (ORF), ORF1 (5643 nt), ORF2 (2121 nt), and ORF3 (348 nt) encoding a nonstructural polyprotein, the major capsid protein precursor, and a minor structural protein, respectively. Phylogenetic analysis of the full-length genome sequence revealed that the Cro1 virus clustered most closely with the VESV species of the genus <it>Vesivirus</it>, but was genetically distinct (82-83% identities with closest strains).</p> <p>Conclusions</p> <p>This is the first description of a full-length genome sequence from a reptile calicivirus (Cro1). The availability of the Cro1 genome sequence should facilitate investigation of the molecular mechanisms involved in Cro1 virus evolution and host range.</p> http://www.virologyj.com/content/9/1/297Reptile calicivirusCro1Complete genomeVesivirus phylogeny |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sandoval-Jaime Carlos Parra Gabriel I Smith Alvin W Green Kim Y Sosnovtsev Stanislav V |
spellingShingle |
Sandoval-Jaime Carlos Parra Gabriel I Smith Alvin W Green Kim Y Sosnovtsev Stanislav V Genetic characterization of a reptilian calicivirus (Cro1) Virology Journal Reptile calicivirus Cro1 Complete genome Vesivirus phylogeny |
author_facet |
Sandoval-Jaime Carlos Parra Gabriel I Smith Alvin W Green Kim Y Sosnovtsev Stanislav V |
author_sort |
Sandoval-Jaime Carlos |
title |
Genetic characterization of a reptilian calicivirus (Cro1) |
title_short |
Genetic characterization of a reptilian calicivirus (Cro1) |
title_full |
Genetic characterization of a reptilian calicivirus (Cro1) |
title_fullStr |
Genetic characterization of a reptilian calicivirus (Cro1) |
title_full_unstemmed |
Genetic characterization of a reptilian calicivirus (Cro1) |
title_sort |
genetic characterization of a reptilian calicivirus (cro1) |
publisher |
BMC |
series |
Virology Journal |
issn |
1743-422X |
publishDate |
2012-11-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Vesiviruses in the family <it>Caliciviridae</it> infect a broad range of animal hosts including mammals, birds, fish, amphibians and reptiles. The vesivirus Cro1 strains were isolated from diseased snakes in the San Diego zoo in 1978 and reported as the first caliciviruses found in reptiles. The goal of this study was to characterize the Cro1 strain 780032I that was isolated in cell culture from a rock rattlesnake (<it>Crotalus lepidus)</it> in the original outbreak.</p> <p>Results</p> <p>We re-amplified the original virus stock in Vero cells, and determined its full-length genome sequence. The Cro1 genome is 8296 nucleotides (nt) in length and has a typical vesivirus organization, with three open reading frames (ORF), ORF1 (5643 nt), ORF2 (2121 nt), and ORF3 (348 nt) encoding a nonstructural polyprotein, the major capsid protein precursor, and a minor structural protein, respectively. Phylogenetic analysis of the full-length genome sequence revealed that the Cro1 virus clustered most closely with the VESV species of the genus <it>Vesivirus</it>, but was genetically distinct (82-83% identities with closest strains).</p> <p>Conclusions</p> <p>This is the first description of a full-length genome sequence from a reptile calicivirus (Cro1). The availability of the Cro1 genome sequence should facilitate investigation of the molecular mechanisms involved in Cro1 virus evolution and host range.</p> |
topic |
Reptile calicivirus Cro1 Complete genome Vesivirus phylogeny |
url |
http://www.virologyj.com/content/9/1/297 |
work_keys_str_mv |
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