Comparison of Salivary Beta Glucuronidase Activity in Chronic Periodontitis Patients with and without Diabetes Mellitus

• Main Authors: Jaiganesh Ramamurthy, Jayakumar ND, Sheeja Varghese
• Format: Article
• Language: English
• Published: JCDR Research and Publications Private Limited 2014-06-01
• Series: Journal of Clinical and Diagnostic Research
• Subjects: beta glucuronidase; diabetes mellitus; periodontitis; saliva
• Online Access: https://jcdr.net/articles/PDF/4476/8713_CE(Ra1)_F(H)_PF1(PAK)_PFA(AK)_PF2(AGAK)_PF2(PN).pdf

Aim of the study: The aim of the study was to estimate the salivary beta glucuronidase (β) activity in patients with chronic periodontitis with and without diabetes mellitus and to evaluate the relationship between Beta Glucuronidase activity and Periodontal clinical parameters. Materials and Methods: The study consisted of 80 patients of both sexes with age ranging from 20-60 years and they were divided into four groups. Clinical parameters such as Gingival index, Probing depth and Clinical attachment loss were measured. Salivary Beta Glucuronidase activity was measured using spectrophotometer with reagents like phenolphthalein glucuronic acid, phosphate and glycine buffer. Results: The mean BG activity of Group IV (1.17 ± 0.27) was significantly higher than mean BGA levels of Group I, II, III. The p-value was < 0.05. The mean BGA levels of Group III (0.78 ± 0.17) was significantly higher than mean BGA levels of Group I, Group II at 5 % level. There was a significant positive linear relationship between salivary β Glucuronidase level and Probing Depth, clinical attachment level in the experimental Groups. Conclusion: The salivary β Glucuronidase level was higher in Diabetic patients with periodontitis than nondiabetic periodontitis patients.