Dysregulation of miR-210 is involved in the development of diabetic retinopathy and serves a regulatory role in retinal vascular endothelial cell proliferation
Abstract Background Diabetic retinopathy is a common complication of diabetes mellitus (DM). The purpose of this study was to investigate the expression and clinical significance of miR-210 in DR patients and explore the regulatory effect of miR-210 on vascular endothelial cell function under high-g...
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doaj-a7640b52335e4cc88e9adabd2ba244bf2020-11-25T03:31:23ZengBMCEuropean Journal of Medical Research2047-783X2020-05-012511810.1186/s40001-020-00416-3Dysregulation of miR-210 is involved in the development of diabetic retinopathy and serves a regulatory role in retinal vascular endothelial cell proliferationChengyu Yin0Xiangqiang Lin1Yafei Sun2Xinli Ji3Department of Ophthalmology, Qingdao Chengyang People’s HospitalDepartment of Ophthalmology, Qingdao Chengyang People’s HospitalDepartment of Ophthalmology, Qingdao Chengyang People’s HospitalDepartment of Ophthalmology, Qingdao Chengyang People’s HospitalAbstract Background Diabetic retinopathy is a common complication of diabetes mellitus (DM). The purpose of this study was to investigate the expression and clinical significance of miR-210 in DR patients and explore the regulatory effect of miR-210 on vascular endothelial cell function under high-glucose condition. Methods Quantitative real-time PCR was used to estimate miR-210 expression. A receiver operating characteristics curve (ROC) was plotted to evaluate the diagnostic value of miR-210. Human umbilical vein endothelial cells (HUVECs) were used and treated with high glucose (30 mM), and the cell proliferation was assessed by MTT assay. Results Serum expression of miR-210 was upregulated in DR patients compared with DM without DR patients and healthy controls. The expression of miR-210 in proliferative DR (PDR) patients was higher than non-proliferative DR (NPDR) patients. The increased serum miR-210 could be used to distinguish DR cases from healthy individuals and also simple DM patients, and can screen PDR cases from NPDR cases. The overexpression of miR-210 promoted HUVEC proliferation, while the knockdown of miR-210 resulted in the opposite effect under a high-glucose condition. Conclusion The data of this study demonstrated that serum increased miR-210 serves as a diagnostic biomarker in DR patients and may have the ability to predict DR development and severity. The regulatory effect of miR-210 on vascular endothelial cell proliferation under high-glucose condition, indicating its therapeutic potential in the treatment of diabetic vascular diseases.http://link.springer.com/article/10.1186/s40001-020-00416-3Diabetic retinopathyMicroRNA-210Diabetes mellitusDiagnosisVascular endothelial cellProliferation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chengyu Yin Xiangqiang Lin Yafei Sun Xinli Ji |
spellingShingle |
Chengyu Yin Xiangqiang Lin Yafei Sun Xinli Ji Dysregulation of miR-210 is involved in the development of diabetic retinopathy and serves a regulatory role in retinal vascular endothelial cell proliferation European Journal of Medical Research Diabetic retinopathy MicroRNA-210 Diabetes mellitus Diagnosis Vascular endothelial cell Proliferation |
author_facet |
Chengyu Yin Xiangqiang Lin Yafei Sun Xinli Ji |
author_sort |
Chengyu Yin |
title |
Dysregulation of miR-210 is involved in the development of diabetic retinopathy and serves a regulatory role in retinal vascular endothelial cell proliferation |
title_short |
Dysregulation of miR-210 is involved in the development of diabetic retinopathy and serves a regulatory role in retinal vascular endothelial cell proliferation |
title_full |
Dysregulation of miR-210 is involved in the development of diabetic retinopathy and serves a regulatory role in retinal vascular endothelial cell proliferation |
title_fullStr |
Dysregulation of miR-210 is involved in the development of diabetic retinopathy and serves a regulatory role in retinal vascular endothelial cell proliferation |
title_full_unstemmed |
Dysregulation of miR-210 is involved in the development of diabetic retinopathy and serves a regulatory role in retinal vascular endothelial cell proliferation |
title_sort |
dysregulation of mir-210 is involved in the development of diabetic retinopathy and serves a regulatory role in retinal vascular endothelial cell proliferation |
publisher |
BMC |
series |
European Journal of Medical Research |
issn |
2047-783X |
publishDate |
2020-05-01 |
description |
Abstract Background Diabetic retinopathy is a common complication of diabetes mellitus (DM). The purpose of this study was to investigate the expression and clinical significance of miR-210 in DR patients and explore the regulatory effect of miR-210 on vascular endothelial cell function under high-glucose condition. Methods Quantitative real-time PCR was used to estimate miR-210 expression. A receiver operating characteristics curve (ROC) was plotted to evaluate the diagnostic value of miR-210. Human umbilical vein endothelial cells (HUVECs) were used and treated with high glucose (30 mM), and the cell proliferation was assessed by MTT assay. Results Serum expression of miR-210 was upregulated in DR patients compared with DM without DR patients and healthy controls. The expression of miR-210 in proliferative DR (PDR) patients was higher than non-proliferative DR (NPDR) patients. The increased serum miR-210 could be used to distinguish DR cases from healthy individuals and also simple DM patients, and can screen PDR cases from NPDR cases. The overexpression of miR-210 promoted HUVEC proliferation, while the knockdown of miR-210 resulted in the opposite effect under a high-glucose condition. Conclusion The data of this study demonstrated that serum increased miR-210 serves as a diagnostic biomarker in DR patients and may have the ability to predict DR development and severity. The regulatory effect of miR-210 on vascular endothelial cell proliferation under high-glucose condition, indicating its therapeutic potential in the treatment of diabetic vascular diseases. |
topic |
Diabetic retinopathy MicroRNA-210 Diabetes mellitus Diagnosis Vascular endothelial cell Proliferation |
url |
http://link.springer.com/article/10.1186/s40001-020-00416-3 |
work_keys_str_mv |
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